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Mouse Podocalyxin APC-conjugated Antibody

Catalog #: FAB1556A
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB1556A
Detection of Podocalyxin in D3 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Podocalyxin APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Podocalyxin in direct ELISAs and Western blots.
Source
Monoclonal Rat IgG2B Clone # 192703
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Podocalyxin
Ser21-Arg402
Accession # Q9R0M4
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Podocalyxin antibody in D3 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Podocalyxin in D3 Mouse Cell Line by Flow Cytometry. Undifferentiated D3 mouse embryonic stem cell line was stained with Rat Anti-Mouse Podocalyxin APC‑conjugated Monoclonal Antibody (Catalog # FAB1556A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of Podocalyxin in bEND-3 cells by Flow Cytometry. bEND-3 cells were stained with Rat Anti-Mouse Podocalyxin APC‑conjugated Monoclonal Antibody (Catalog # FAB1556A, filled histogram) or isotype control antibody (Catalog # IC013A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Podocalyxin

Podocalyxin, also known as Podocalyxin-like protein-1 (PCLP1 or PODXL), is a type I transmembrane glycoprotein. It belongs to the CD34/Podocalyxin family of sialomucins that share structural similarity and sequence homology. Podocalyxin is a major sialoprotein in the podocytes of the kidney glomerulus and is also expressed by both endothelium and multipotent hematopoietic progenitors. It has been identified as a novel cell surface marker for hemangioblasts, the common precursors of hematopoietic and endothelial cells (1, 2).

References
  1. Li, J. et al. (2001) DNA Seq. 12(5):407.
  2. Hara, T. et al. (1999) Immunity 11(5):567.
Entrez Gene IDs
5420 (Human); 27205 (Mouse)
Alternate Names
GCTM; Gp200MGC138240; PCLP; PCLP1; PCLP-1; PCLPGCTM-2 antigen; PCpodocalyxin; POD XL; Podocalyxin; Podocalyxin-like protein 1; podocalyxin-like; PODXL

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Citation for Mouse Podocalyxin APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Maintenance of Mouse Nephron Progenitor Cells in Aggregates with Gamma-Secretase Inhibitor.
    Authors: Yuri S, Nishikawa M, Yanagawa N, Jo O, Yanagawa N
    PLoS ONE, 2015-06-15;10(6):e0129242.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC

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