Mouse PRDM14 Antibody

PRDM14 in D3 Mouse Embryonic Stem Cells. PRDM14 was detected in immersion fixed D3 mouse embryonic stem cells using Rat Anti-Mouse PRDM14 Monoclonal Antibody (Catalog # MAB8097) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Detection of Mouse PRDM14 by Western Blot PRDM14 Induces the Transition of EpiLCs to ESCLCs(A) Constructs of the ROSA-Tet-off system and piggyBac Tet-on system.(B) Western blotting of PRDM14 and tubulin.(C) Scheme of the transition of ESCs to EpiLCs and of EpiLCs to ESCLCs.(D) qRT-PCR analysis of Prdm14 expression in ESCs, EpiLCs (d2), EpiLCs (d4), and EpiLCs + PRDM14. Error bars indicate ±SD of a biological triplicate.(E) AP staining of ESCs, EpiLCs (d2), EpiLCs (d4), and EpiLCs + PRDM14. Scale bar, 50 μm.(F) qRT-PCR analysis of pluripotency genes in ESCs, EpiLCs (d2), EpiLCs (d4), and EpiLCs + PRDM14. Error bars indicate ±SD of a biological triplicate.(G) Colony formation by ESCs, EpiLCs (d4), and EpiLCs + PRDM14 was assessed by AP staining over 3 days in the presence of serum + LIF. Error bars indicate ±SD of biological triplicates. p Values were calculated with the Student's t test. Scale bar, 200 μm.(H) (Left) Teratoma formation by EpiLCs expressing Prdm14 cultured in the absence of LIF. (Right) Immunofluorescent staining of alpha -fetoprotein (AFP), smooth muscle actin (SMA), and class III beta -tubulin (TUJ1) in teratoma sections. Scale bar, 200 μm.∗∗p < 0.01, ∗∗∗p < 0.001, Student's t test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/27866876), licensed under a CC-BY license. Not internally tested by R&D Systems.