Mouse Pref-1/DLK1/FA1 APC-conjugated Antibody

Catalog #: FAB8634A
4 Citations Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
FAB8634A-025
FAB8634A-100

Detection of Pref‑1/DLK1/FA1 in 3T3‑L1 Mouse Cell Line by Flow Cytometry.

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Pref-1/DLK1/FA1 APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Pref-1/DLK1/FA1 in direct ELISAs.

Source

Recombinant Monoclonal Rabbit IgG Clone # 1168B

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Pref-1/DLK1/FA1
Ala24-Gln305
Accession # Q09163

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Pref‑1/DLK1/FA1 in 3T3‑L1 Mouse Cell Line by Flow Cytometry. 3T3-L1 mouse embryonic fibroblast adipose-like cell line was stained with Rabbit Anti-Mouse Pref-1/DLK1/FA1 APC-conjugated Monoclonal Antibody (Catalog # FAB8634A, filled histogram) or isotype control antibody (Catalog # IC105A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Pref-1/DLK1/FA1

Pref-1 (Preadipocyte factor 1), also known as Protein delta homolog 1, DLK1, FA1 and Fetal antigen 1, is a 45-60 kDa transmembrane glycoprotein that is highly expressed in fetal liver, placenta, adult adrenal gland, brain, testis and ovary. Expression of Pref-1 is elevated in liver after birth but starts to decline around postnatal day 16. Mature mouse Pref-1 is a 362 amino acid (aa) type I transmembrane N- and O-linked glycoprotein. It contains a 282 aa extracellular region (aa 24-305), a 24 aa transmembrane segment (aa 306-329), and a 56 aa cytoplasmic domain (aa 330-385). It contains 6 EGF-like domains and is involved in embryonic skeletal system development. Pref-1 inhibits preadipocyte proliferation by regulating their entry into G1/S-phase and represses preadipocyte differentiation. It is a master regulator of preadipocyte homeostasis and adipose tissue expansion. Pref-1 manipulation may, therefore, be utilized in obesity treatments. Mouse Pref-1 shares 85% and 94% aa identity with human and rat Pref-1, respectively.

Long Name

Preadipocyte Factor-1/Protein delta Homolog 1/Fetal Antigen 1

Entrez Gene IDs

8788 (Human); 13386 (Mouse)

Alternate Names

delta-like 1 homolog (Drosophila); DLK; DLK1; DLK-1; FA1; FA1fetal antigen 1; pG2; pG2delta-like homolog (Drosophila); preadipocyte factor 1; Pref1; Pref-1; protein delta homolog 1; secredeltin; ZOG

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Mouse Pref-1/DLK1/FA1 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Tcf21 marks visceral adipose mesenchymal progenitors and functions as a rate-limiting factor during visceral adipose tissue development
Authors: Q Liu, C Li, B Deng, P Gao, L Wang, Y Li, M Shiri, F Alkaifi, J Zhao, JM Stephens, CA Simintiras, J Francis, J Sun, X Fu
Cell Reports, 2023-02-28;42(3):112166.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Prrx1 Fibroblasts Represent a Pro-fibrotic Lineage in the Mouse Ventral Dermis
Authors: T Leavitt, MS Hu, MR Borrelli, M Januszyk, JT Garcia, RC Ransom, S Mascharak, HE desJardins, UM Litzenburg, GG Walmsley, CD Marshall, AL Moore, B Duoto, S Adem, DS Foster, A Salhotra, AH Shen, M Griffin, EZ Shen, LA Barnes, ER Zielins, ZN Maan, Y Wei, CKF Chan, DC Wan, HP Lorenz, HY Chang, GC Gurtner, MT Longaker
Cell Rep, 2020-11-10;33(6):108356.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
Single-cell RNA-Seq analysis reveals dynamic trajectories during mouse liver development
Authors: X Su, Y Shi, X Zou, ZN Lu, G Xie, JYH Yang, CC Wu, XF Cui, KY He, Q Luo, YL Qu, N Wang, L Wang, ZG Han
BMC Genomics, 2017-12-04;18(1):946.
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry
The antifibrotic adipose-derived stromal cell: Grafted fat enriched with CD74+ adipose-derived stromal cells reduces chronic radiation-induced skin fibrosis
Authors: Mimi R. Borrelli, Ronak A. Patel, Sandeep Adem, Nestor M. Diaz Deleon, Abra H. Shen, Jan Sokol et al.
Stem Cells Translational Medicine