Mouse Protogenin Antibody

Catalog #: AF4919 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4919
AF4919-SP

Protogenin in Embryonic Mouse Neural Tube.

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Mouse Protogenin Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse and human PRTG in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse PRTG
Val24-Thr943
Accession # Q2EY15

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Protogenin

Immunohistochemistry

5-15 µg/mL

Immersion fixed frozen sections of embryonic mouse neural tube (E9) and immersion fixed paraffin-embedded 13.5 d.p.c sections of Mouse Embryo

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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Protogenin in Embryonic Mouse Neural Tube. Protogenin was detected in immersion fixed frozen sections of embryonic mouse neural tube (E9) using 10 µg/mL Sheep Anti-Mouse Protogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4919) overnight at 4 °C. Tissue was stained with the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunohistochemistry

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Protogenin in Mouse Neural Tube. Protogenin was detected in immersion fixed frozen sections of mouse neural tube (E9.5) using Sheep Anti-Mouse Protogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4919) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (yellow; NL010) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Immunohistochemistry

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Detection of Protogenin in Mouse Embryo. Protogenin was detected in immersion fixed paraffin-embedded 13.5 d.p.c sections of Mouse Embryo using Sheep Anti-Mouse Protogenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4919) at 10 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Sheep IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC006). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell membranes in neuronal cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protogenin

Protogenin (Early/proto in expression, neogenin-like; also Prtg) is a 130 kDa (predicted), type I transmembrane member of the DCC/Neogenin family of proteins. It is expressed in mouse presomitic mesoderm, paraxial mesoderm, and neural tube. Mature mouse protogenin is 1168 amino acids (aa) in length. It contains a 920 aa extracellular domain (ECD) (aa 24-943) plus a 224 aa cytoplasmic region. The ECD contains four consecutive Ig-like domains (aa 24-405), followed by five sequential fibronectin type III domains (aa 413-908). The ECD of mouse protogenin shares 98%, 87%, and 93% aa sequence identity with the ECD of rat, chick, and human protogenin, respectively.

Entrez Gene IDs

283659 (Human); 235472 (Mouse); 315806 (Rat)

Alternate Names

FLJ12795; FLJ13221; FLJ25756; IGDCC5; immunoglobulin superfamily, DCC subclass, member 5; Protein Shen-Dan; protogenin homolog (Gallus gallus); Protogenin; PRTG; RPTG; Shen-Dan