Mouse Rae-1 Pan Specific Antibody

Rae‑1 in Mouse Embryo. Rae-1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Rae-1 Pan Specific Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1136) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to axons of primary sensory neurons. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Detection of Mouse Rae-1 by Western Blot Sciatic Nerve Crush Increases RAE1 in Peripheral Nerve Axons(A) Schematic diagram of sciatic nerve crush injury site in relation to lumbar DRGs (L3, L4, and L5).(B) Raet1 mRNA expression in ipsilateral L3–5 DRG 3 and 7 days after surgery by qPCR. Two-way ANOVA: effect of injury, F(1,8) = 180.95, p < 0.0001; effect of time, F(1,8) = 8.04, p = 0.0220. Bonferroni post-test: 3 days, t = 7.507, ∗∗∗p < 0.001; 7 days, t = 11.52, ∗∗∗p < 0.001. n = 3 mice per surgery per time point.(C) In situ hybridization with Raet1 probe (red) in L4 DRG immunolabeled for NeuN (blue) 6 days after partial sciatic nerve crush injury. Scale bar, 50 μm.(D) Distribution of Raet1 in situ spots in L4 DRG ipsi and contralateral to sciatic crush. Kolmogorov-Smirnov (KS) test: ∗∗∗p < 0.0001, KS D-value 0.2297. n = 3 mice, n = 3 sections per ipsi and contralateral DRG per mouse.(E) (Top) Pan-RAE1 western blot of sciatic nerve tissue (40 μg protein loading) 3 days after sciatic nerve crush or sham surgery. Neuronal cadherin control is shown. (Bottom) Relative expression of RAE1 protein in ipsilateral versus contralateral sciatic nerves 3 and 7 days after sham or crush surgery. One-way ANOVA, 3 days: F(3,16) = 8.505, p = 0.0013. Bonferroni post-test: sham ipsi versus contra, t = 0.2025, ns p > 0.05; crush ipsi versus contra, t = 3.678, ∗p < 0.05; ipsi sham versus crush, t = 4.438, ∗∗p < 0.01. One-way ANOVA, 7 days: F(3,16) = 5.119, p = 0.0113. Bonferroni post-test: sham ipsi versus contra, t = 0.1438, ns p > 0.05; crush ipsi versus contra, t = 3.342, ∗p < 0.05; ipsi sham versus crush, t = 3.043, ∗p < 0.05 (n = 5 mice per surgery group per time point).(F) (Top) Pan-RAE1 western blot of DRG (L3–5) tissue (40 μg protein loading) 3 days after sciatic nerve crush or sham surgery. Neuronal cadherin control. (Bottom) Relative expression of RAE1 protein in ipsilateral versus contralateral DRG 3 and 7 days after sham or crush surgery. One-way ANOVA: 3 days, F(3,16) = 0.438, p = 0.7288; 7 days, F(3,20) = 0.053, p = 0.983 (n = 5–6 mice per surgery group per time point).(G) Composite images of RAE1 immunolabeling in full-length contralateral and ipsilateral sciatic nerves taken from three individual mice 3 days after tight sciatic nerve ligation. Arrow, ligation site; arrowhead, proximal to ligation.(H) Maximum projection images of RAE1 (green) co-localization with axonal marker beta -tubulin III (magenta) in sciatic nerve 3 days after tight ligation. Scale bars, 50 μm.See also Figures S3 and S4. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30712871), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse Rae-1 by Western Blot Sciatic Nerve Crush Increases RAE1 in Peripheral Nerve Axons(A) Schematic diagram of sciatic nerve crush injury site in relation to lumbar DRGs (L3, L4, and L5).(B) Raet1 mRNA expression in ipsilateral L3–5 DRG 3 and 7 days after surgery by qPCR. Two-way ANOVA: effect of injury, F(1,8) = 180.95, p < 0.0001; effect of time, F(1,8) = 8.04, p = 0.0220. Bonferroni post-test: 3 days, t = 7.507, ∗∗∗p < 0.001; 7 days, t = 11.52, ∗∗∗p < 0.001. n = 3 mice per surgery per time point.(C) In situ hybridization with Raet1 probe (red) in L4 DRG immunolabeled for NeuN (blue) 6 days after partial sciatic nerve crush injury. Scale bar, 50 μm.(D) Distribution of Raet1 in situ spots in L4 DRG ipsi and contralateral to sciatic crush. Kolmogorov-Smirnov (KS) test: ∗∗∗p < 0.0001, KS D-value 0.2297. n = 3 mice, n = 3 sections per ipsi and contralateral DRG per mouse.(E) (Top) Pan-RAE1 western blot of sciatic nerve tissue (40 μg protein loading) 3 days after sciatic nerve crush or sham surgery. Neuronal cadherin control is shown. (Bottom) Relative expression of RAE1 protein in ipsilateral versus contralateral sciatic nerves 3 and 7 days after sham or crush surgery. One-way ANOVA, 3 days: F(3,16) = 8.505, p = 0.0013. Bonferroni post-test: sham ipsi versus contra, t = 0.2025, ns p > 0.05; crush ipsi versus contra, t = 3.678, ∗p < 0.05; ipsi sham versus crush, t = 4.438, ∗∗p < 0.01. One-way ANOVA, 7 days: F(3,16) = 5.119, p = 0.0113. Bonferroni post-test: sham ipsi versus contra, t = 0.1438, ns p > 0.05; crush ipsi versus contra, t = 3.342, ∗p < 0.05; ipsi sham versus crush, t = 3.043, ∗p < 0.05 (n = 5 mice per surgery group per time point).(F) (Top) Pan-RAE1 western blot of DRG (L3–5) tissue (40 μg protein loading) 3 days after sciatic nerve crush or sham surgery. Neuronal cadherin control. (Bottom) Relative expression of RAE1 protein in ipsilateral versus contralateral DRG 3 and 7 days after sham or crush surgery. One-way ANOVA: 3 days, F(3,16) = 0.438, p = 0.7288; 7 days, F(3,20) = 0.053, p = 0.983 (n = 5–6 mice per surgery group per time point).(G) Composite images of RAE1 immunolabeling in full-length contralateral and ipsilateral sciatic nerves taken from three individual mice 3 days after tight sciatic nerve ligation. Arrow, ligation site; arrowhead, proximal to ligation.(H) Maximum projection images of RAE1 (green) co-localization with axonal marker beta -tubulin III (magenta) in sciatic nerve 3 days after tight ligation. Scale bars, 50 μm.See also Figures S3 and S4. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30712871), licensed under a CC-BY license. Not internally tested by R&D Systems.