Mouse/Rat BCAM Antibody Summary
Met1-Ala541
Accession # Q9R069
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse and Rat BCAM by Western Blot. Western blot shows lysates of mouse kidney tissue, mouse skeletal muscle tissue, rat heart tissue, and rat lung tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat BCAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8299) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for BCAM at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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BCAM in Mouse Liver. BCAM was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse/Rat BCAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8299) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to endothelial cells. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BCAM
Mouse BCAM (Basal cell adhesion molecule, B-CAM cell surface glycoprotein,Lutheran antigen, and CD239) is a 70-90 kDa membrane glycoprotein. BCAM has wide tissue distribution and is expressed on erythrocytes, the endothelium of blood vessels and on the basal layer of cells in the epithelia. The expression of BCAM in normal tissues is higher in fetal versus adult tissues. BCAM expression is also upregulated in activated keratinocytes and following malignant transformation in some cell types in vivo and in vitro. BCAM has been shown to be an adhesion molecule that binds laminin, a basement membrane protein involved in cell differentiation, adhesion, migration and proliferation. Over aa 1-541, mouse BCAM is 91% identical to rat BCAM.
Product Datasheets
Citations for Mouse/Rat BCAM Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Apoptosis and tissue thinning contribute to symmetric cell division in the developing mouse epidermis in a nonautonomous way
Authors: A Soffer, A Mahly, K Padmanabha, J Cohen, O Adir, E Loushi, Y Fuchs, SE Williams, C Luxenburg
PloS Biology, 2022-08-15;20(8):e3001756.
Species: Mouse
Sample Types: Cell Lysates, Whole Tissue
Applications: IF, Western Blot -
Induction of osteogenesis by bone-targeted Notch activation
Authors: C Xu, VV Dinh, K Kruse, HW Jeong, EC Watson, S Adams, F Berkenfeld, M Stehling, SJ Rasouli, R Fan, R Chen, I Bedzhov, Q Chen, K Kato, ME Pitulescu, RH Adams
Elife, 2022-02-04;11(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Extensive remodeling of the extracellular matrix during aging contributes to age-dependent impairments of muscle stem cell functionality
Authors: SC Schüler, JM Kirkpatric, M Schmidt, D Santinha, P Koch, S Di Sanzo, E Cirri, M Hemberg, A Ori, J von Maltza
Cell Reports, 2021-06-08;35(10):109223.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Cell-matrix signals specify bone endothelial cells during developmental osteogenesis
Authors: UH Langen, ME Pitulescu, JM Kim, R Enriquez-G, KK Sivaraj, AP Kusumbe, A Singh, J Di Russo, MG Bixel, B Zhou, L Sorokin, JM Vaquerizas, RH Adams
Nat. Cell Biol, 2017-02-20;19(3):189-201.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC
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