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Mouse/Rat cAMP Parameter Assay Kit

Catalog #: KGE012B
35 Citations 2 Reviews Datasheet / COA / SDS
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KGE012B
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QC53: Parameter Immunoassay Control Set 1165 for Mouse/Rat cAMP
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Mouse/Rat cAMP ELISA Standard Curve
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Citations (35)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse/Rat cAMP Parameter Assay Kit Summary

Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Tissue Homogenates (50 uL), EDTA Plasma (50 uL), Urine (10 uL)
Sensitivity
1.54 nmol/L
Assay Range
3.8 - 240 nmol/L (Cell Culture Supernates, Cell Lysates, Tissue Homogenates, EDTA Plasma, Urine)
Specificity
Cyclic AMP in mouse and rat samples
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The R&D Systems cAMP Immunoassay is a 3.5 hour competitive enzyme immunoassay designed to measure cAMP in cell culture supernates, cell lysates, tissue homogenates, plasma, and urine.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Cell Lysates, Tissue Homogenates, EDTA Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (nmol/L) 27.7 58.7 96.9 28.2 61.7 102
Standard Deviation 0.5 1 3.9 2.2 4.8 6.5
CV% 1.8 1.7 4 7.8 7.8 6.4

Recovery

The recovery of cAMP spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Samples (n=4) 100 98-103
Cell Lysates (n=2) 106 106-107
Mouse EDTA Plasma (n=4) 97 92-101
Rat EDTA Plasma (n=4) 104 101-108
Tissue Homogenates (n=4) 110 105-115

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of cAMP were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Mouse/Rat cAMP ELISA Linearity

Scientific Data

N/A Mouse/Rat cAMP ELISA Standard Curve View Larger

Mouse/Rat cAMP ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: cAMP

Adenosine 3,5-cyclic monophosphate (cAMP) is one of the most important intracellular second messengers. Several hormones are known to activate cAMP and subsequent signaling cascades via their indirect influence on adenylate cyclase, the enzyme responsible for the conversion of ATP to cAMP.

Long Name:
Cyclic AMP
Alternate Names:
cAMP; Cyclic AMP
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Mouse/Rat cAMP Parameter Assay Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
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  1. Adenosine metabolized from extracellular ATP ameliorates organ injury by triggering A2BR signaling
    Authors: Kelestemur, T;Németh, ZH;Pacher, P;Beesley, J;Robson, SC;Eltzschig, HK;Haskó, G;
    Respiratory research
    Species: Mouse
    Sample Types: BALF
  2. Anti-Melanogenic Effects of Fractioned Cynanchum atratum by Regulation of cAMP/MITF Pathway in a UVB-Stimulated Mice Model
    Authors: Wang, JH;Hwang, SJ;Lee, SK;Choi, Y;Byun, CK;Son, CG;
    Cells
    Species: Mouse
    Sample Types: Cell Lysates, Tissue Homogenates
  3. CD47 antisense oligonucleotide treatment attenuates obesity and its-associated metabolic dysfunction
    Authors: T Gwag, D Li, E Ma, Z Guo, Y Liang, S Wang
    Scientific Reports, 2023-02-16;13(1):2748.
    Species: Mouse
    Sample Types: Tissue Homogenates
  4. Icariin Promotes Osteogenic Differentiation in a Cell Model with NF1 Gene Knockout by Activating the cAMP/PKA/CREB Pathway
    Authors: Chen, M;Lu, L;Cheng, D;Zhang, J;Liu, X;Zhang, J;Zhang, T;
    Molecules
    Species: Mouse
    Sample Types: Cell Lysates
  5. A brown fat-enriched adipokine Adissp controls adipose thermogenesis and glucose homeostasis
    Authors: Q Chen, L Huang, D Pan, K Hu, R Li, RH Friedline, JK Kim, LJ Zhu, DA Guertin, YX Wang
    Nature Communications, 2022-12-10;13(1):7633.
    Species: Mouse
    Sample Types: Tissue Lysates
  6. Intrathecal Injection of Ropivacaine Reduces Cervical Resistance in Late-Pregnant Rats
    Authors: Y Hu, LJ Fan, YM Jiang, H Liu, H Yong, C Peng
    Drug Design, Development and Therapy, 2022-04-26;16(0):1183-1189.
    Species: Rat
    Sample Types: Tissue Homogenates
  7. Deiodinase-3 is a thyrostat to regulate podocyte homeostasis
    Authors: S Agarwal, KH Koh, NJ Tardi, C Chen, RR Dande, JP WerneckdeC, YR Sudhini, C Luongo, D Salvatore, B Samelko, MM Altintas, S Mangos, A Bianco, J Reiser
    EBioMedicine, 2021-10-11;72(0):103617.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  8. Gap Junction-Mediated Intercellular Communication of cAMP Prevents CDDP-Induced Ototoxicity via cAMP/PKA/CREB Pathway
    Authors: YJ Kim, JS Lee, H Kim, JH Jang, YH Choung
    International Journal of Molecular Sciences, 2021-06-13;22(12):.
    Species: Mouse
    Sample Types: Cell Lysates
  9. Octreotide and Pasireotide Combination Treatment in Somatotroph Tumor Cells: Predominant Role of SST2 in Mediating Ligand Effects
    Authors: J Amarù, F Barbieri, M Arvigo, A Solari, A Bajetto, F Nista, C Campana, G Gaggero, A Prior, D Criminelli, G Zona, D Ferone, T Florio, F Gatto
    Cancers, 2021-04-10;13(8):.
    Species: Rat
    Sample Types: Cell Lysates
  10. A Novel Follitropin Analog Inhibits Follitropin Activity In Vitro
    Authors: N Azzam, R Bar-Shalom, F Fares
    Pharmaceutics, 2021-03-03;13(3):.
    Species: Hamster
    Sample Types: Cell Culture Supernates
  11. Adipose tissue lipolysis is regulated by PAQR11 via altering protein stability of phosphodiesterase 4D
    Authors: M Huang, Y Lin, L Wang, X You, S Wang, J Zhao, M Bai, Z Li, Y Chen
    Molecular Metabolism, 2021-02-05;47(0):101182.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  12. Structures of the glucocorticoid-bound adhesion receptor GPR97-Go complex
    Authors: YQ Ping, C Mao, P Xiao, RJ Zhao, Y Jiang, Z Yang, WT An, DD Shen, F Yang, H Zhang, C Qu, Q Shen, C Tian, ZJ Li, S Li, GY Wang, X Tao, X Wen, YN Zhong, J Yang, F Yi, X Yu, HE Xu, Y Zhang, JP Sun
    Nature, 2021-01-06;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  13. IL-1beta suppression of VE-cadherin transcription underlies sepsis-induced inflammatory lung injury
    Authors: S Xiong, Z Hong, LS Huang, Y Tsukasaki, S Nepal, A Di, M Zhong, W Wu, Z Ye, X Gao, GN Rao, D Mehta, J Rehman, AB Malik
    J. Clin. Invest., 2020-07-01;130(7):3684-3698.
    Species: Mouse
    Sample Types: Cell Lysates
  14. R848 or influenza virus can induce potent innate immune responses in the lungs of neonatal mice
    Authors: S Makris, C Johansson
    Mucosal Immunol, 2020-06-23;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  15. Acetate attenuates inflammasome activation through GPR43-mediated Ca2+-dependent NLRP3 ubiquitination
    Authors: M Xu, Z Jiang, C Wang, N Li, L Bo, Y Zha, J Bian, Y Zhang, X Deng
    Exp. Mol. Med., 2019-07-23;51(7):83.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  16. Regression of fibrosis by cilostazol in a rat model of thioacetamide-induced liver fibrosis: Up regulation of hepatic cAMP, and modulation of inflammatory, oxidative stress and apoptotic biomarkers
    Authors: SA El Awdan, RF Abdel Rahm, HM Ibrahim, RR Hegazy, SA El Marasy, M Badawi, MS Arbid
    PLoS ONE, 2019-05-08;14(5):e0216301.
    Species: Rat
    Sample Types: Tissue Homogenates
  17. Application of a dye-based mitochondrion-thermometry to determine the receptor downstream of prostaglandin E2 involved in the regulation of hepatocyte metabolism
    Authors: L Shen, TR Xie, RZ Yang, Y Chen, JS Kang
    Sci Rep, 2018-08-30;8(1):13065.
    Species: Mouse
    Sample Types: Whole Cells
  18. Vanadate-Induced Renal cAMP and Malondialdehyde Accumulation Suppresses Alpha 1 Sodium Potassium Adenosine Triphosphatase Protein Levels
    Authors: S Eiam-Ong, Y Nakchui, M Chaipipat, S Eiam-Ong
    Toxicol Res, 2018-04-15;34(2):143-150.
    Species: Rat
    Sample Types: Tissue Homogenates
  19. Gq activity- and ?-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility
    Authors: DL Zhang, YJ Sun, ML Ma, YJ Wang, H Lin, RR Li, ZL Liang, Y Gao, Z Yang, DF He, A Lin, H Mo, YJ Lu, MJ Li, W Kong, KY Chung, F Yi, JY Li, YY Qin, J Li, ARB Thomsen, AW Kahsai, ZJ Chen, ZG Xu, M Liu, D Li, X Yu, JP Sun
    Elife, 2018-02-02;7(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
  20. 2-Methoxyestradiol Causes MMP 9 Mediated Transactivation of EGFR and Angiotensin Type 1 Receptor Downregulation in Rat Aortic Smooth Muscle Cells
    Authors: B Ogola, Y Zhang, L Iyer, T Thekkumkar
    Am. J. Physiol., Cell Physiol., 2018-01-24;0(0):.
    Species: Rat
    Sample Types: Cell Lysates
  21. Atlantic salmon (Salmo salar) co-product-derived protein hydrolysates: A source of antidiabetic peptides
    Authors: PA Harnedy, V Parthsarat, CM McLaughlin, MB O'Keeffe, PJ Allsopp, EM McSorley, FPM O'Harte, RJ FitzGerald
    Food Res. Int., 2018-01-16;106(0):598-606.
    Species: Rat
    Sample Types: Cell Lysates
  22. Role of the hippocampal 5-HT1A receptor-mediated cAMP/PKA signalling pathway in sevoflurane-induced cognitivedysfunction in aged rats
    Authors: Y Qiu, Y Wang, X Wang, C Wang, ZY Xia
    J. Int. Med. Res., 2018-01-14;0(0):3000605177440.
    Species: Rat
    Sample Types: Tissue Homogenates
  23. Molecular mechanisms of calcium signaling in the modulation of small intestinal ion transports and bicarbonate secretion
    Authors: X Yang, G Wen, B Tuo, F Zhang, H Wan, J He, S Yang, H Dong
    Oncotarget, 2017-12-11;9(3):3727-3740.
    Species: Mouse
    Sample Types: Protein
  24. Naringenin Regulates CFTR Activation and Expression in Airway Epithelial Cells
    Authors: R Shi, ZT Xiao, YJ Zheng, YL Zhang, JW Xu, JH Huang, WL Zhou, PB Li, WW Su
    Cell. Physiol. Biochem., 2017-11-27;44(3):1146-1160.
    Species: Rat
    Sample Types: Cell Culture Supernates
  25. Targeting G-protein coupled receptor-related signaling pathway in a murine xenograft model of appendiceal pseudomyxoma peritonei
    Authors: AK Dilly, BD Honick, YJ Lee, ZS Guo, HJ Zeh, DL Bartlett, HA Choudry
    Oncotarget, 2017-11-06;8(63):106888-106900.
    Species: Mouse
    Sample Types: Tissue Homogenates
  26. Exendin-4, a glucagon-like peptide-1 analogue accelerates healing of chronic gastric ulcer in diabetic rats
    Authors: YC Chen, CC Ho, CH Yi, XZ Liu, TT Cheng, CF Lam
    PLoS ONE, 2017-11-02;12(11):e0187434.
    Species: Rat
    Sample Types: Tissue Homogenates
  27. Effects of Aluminium on long-term Memory in Rats and on SIRT1 Mediating the Transcription of CREB-Dependent Gene in Hippocampus
    Authors: D Yan, C Jin, Y Cao, L Wang, X Lu, J Yang, S Wu, Y Cai
    Basic Clin. Pharmacol. Toxicol., 2017-06-19;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  28. Molecular signaling underlying bulleyaconitine A (BAA)-induced microglial expression of prodynorphin
    Authors: TF Li, HY Wu, YR Wang, XY Li, YX Wang
    Sci Rep, 2017-03-22;7(0):45056.
    Species: Rat
    Sample Types: Cell Lysates
  29. Increased efficacy of a dendritic cell-based therapeutic cancer vaccine with adenosine receptor antagonist and CD73 inhibitor
    Authors: S Arab, N Kheshtchin, M Ajami, M Ashurpoor, A Safvati, A Namdar, R Mirzaei, N Mousavi Ni, F Jadidi-Nia, MH Ghahremani, J Hadjati
    Tumour Biol, 2017-03-01;39(3):1010428317695.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  30. p38? MAPK signaling mediates exenatide-stimulated microglial ?-endorphin expression
    Authors: HY Wu, XF Mao, H Fan, YX Wang
    Mol. Pharmacol, 2017-02-15;0(0):.
    Species: Rat
    Sample Types: Cell Lysates
  31. Inhibition of Adenylyl Cyclase in the Spinal Cord Alleviates Painful Diabetic Neuropathy in Zucker Diabetic Fatty Rats
    Authors: Zhonghao Liu
    Can J Diabetes, 2016-11-23;0(0):.
    Species: Rat
    Sample Types: Tissue Homogenates
  32. Chronic Repression of mTOR Complex 2 Induces Changes in the Gut Microbiota of Diet-induced Obese Mice
    Sci Rep, 2016-07-29;6(0):30887.
    Species: Mouse
    Sample Types: Tissue Homogenates
  33. MicroRNA-378 controls classical brown fat expansion to counteract obesity.
    Authors: Pan, Dongning, Mao, Chunxiao, Quattrochi, Brian, Friedline, Randall, Zhu, Lihua J, Jung, Dae Youn, Kim, Jason K, Lewis, Brian, Wang, Yong-Xu
    Nat Commun, 2014-08-22;5(0):4725.
    Species: Mouse
    Sample Types: Tissue Homogenates
  34. Neurofibromatosis type 1 alternative splicing is a key regulator of Ras signaling in neurons.
    Authors: Hinman M, Sharma A, Luo G, Lou H
    Mol Cell Biol, 2014-04-07;34(12):2188-97.
    Species: Mouse
    Sample Types: Cell Lysates
  35. Resolvin D1 stimulates alveolar fluid clearance through alveolar epithelial sodium channel, Na,K-ATPase via ALX/cAMP/PI3K pathway in lipopolysaccharide-induced acute lung injury.
    Authors: Wang Q, Zheng X, Cheng Y, Zhang Y, Wen H, Tao Z, Li H, Hao Y, Gao Y, Yang L, Smith F, Huang C, Jin S
    J Immunol, 2014-03-19;192(8):3765-77.
    Species: Rat
    Sample Types: Cell Lysates

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Mouse/Rat cAMP Parameter Assay Kit
By Anonymous on 03/30/2023
Sample Tested: Kidney tissue
Mouse/Rat cAMP Parameter Assay Kit
By Anonymous on 07/24/2017
Sample Tested: CHO Chinese hamster ovary cell line