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Mouse/Rat CCR10 APC-conjugated Antibody

Catalog #: FAB2815A
7 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB2815A-025
FAB2815A-100
Detection of CCR10 in B16‑F1 Mouse Cell Line by Flow Cytometry.
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Product Details
Citations (7)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat CCR10 APC-conjugated Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat CCR10. Stains mouse CCR10-transfected cells but not irrelevant transfectants.
Source
Monoclonal Rat IgG2A Clone # 248918
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
C6 rat glioma cell line transfected with mouse CCR10
Met1-Asn362
Accession # NP_031747
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
B16‑F1 mouse melanoma cell line, Raw264.7 cell line, and rat splenocytes, HEK293 cell line transfected with mCCR10

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CCR10 antibody in B16‑F1 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of CCR10 in B16‑F1 Mouse Cell Line by Flow Cytometry. B16-F1 mouse melanoma cell line was stained with Rat Anti-Mouse/Rat CCR10 APC-conjugated Monoclonal Antibody (Catalog # FAB2815A, filled histogram) or isotype control antibody (IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of CCR10 antibody in Rat Splenocytes antibody by Flow Cytometry. View Larger

Detection of CCR10 in Rat Splenocytes by Flow Cytometry. Rat splenocytes were stained with Rat Anti-Mouse/Rat CCR10 APC-conjugated Monoclonal Antibody (Catalog # FAB2815A) and Anti-Rat CD3 PE-conjugated Antibody. Quadrant markers were set based on control antibody staining (IC006A). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of CCR10 in Raw264.7 cells by Flow Cytometry. Raw264.7 cells were stained with Rat Anti-Mouse/Rat CCR10 APC‑conjugated Monoclonal Antibody (Catalog # FAB2815A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry View Larger

Detection of CCR10 in HEK293 Human Cell Line transfected with Mouse CCR10 and eGFP vs irrelevant GPCR by Flow Cytometry HEK293 Human Cell Line transfected with Mouse CCR10 and eGFP (A) vs irrelevant GPCR (B) were stained with Rat Anti-Mouse/Rat CCR10 APC‑conjugated Monoclonal Antibody (Catalog # fab2815a) and eGFP isotype control antibody (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR10

CCR10 is a G protein-linked seven transmembrane domain protein expressed by T cells and B cell subsets that functions as a receptor for CCL27. CCR10 mediates lymphocyte migration to the skin and mucosa, and its expression correlates with the metastatic capacity of melanomas.

Entrez Gene IDs
2826 (Human); 12777 (Mouse); 363682 (Rat)
Alternate Names
CC chemokine receptor 10; C-C chemokine receptor type 10; C-C CKR-10; CC-CKR-10; CCR10; CCR-10; chemokine (C-C motif) receptor 10; G protein-coupled receptor 2; GPR2; GPR2G-protein coupled receptor 2

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Citations for Mouse/Rat CCR10 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Limited clonal relatedness between gut IgA plasma cells and memory B cells after oral immunization
    Authors: Mats Bemark, Helena Hazanov, Anneli Strömberg, Rathan Komban, Joel Holmqvist, Sofia Köster et al.
    Nature Communications
  2. BLT1 mediates commensal bacteria-dependent innate immune signals to enhance antigen-specific intestinal IgA responses
    Authors: Takahiro Nagatake, So-ichiro Hirata, Tomoaki Koga, Etsushi Kuroda, Shingo Kobari, Hidehiko Suzuki et al.
    Mucosal Immunology
  3. The gut microbiota induces Peyer's-patch-dependent secretion of maternal IgA into milk
    Authors: K Usami, K Niimi, A Matsuo, Y Suyama, Y Sakai, S Sato, K Fujihashi, H Kiyono, S Uchino, M Furukawa, J Islam, K Ito, T Moriya, Y Kusumoto, M Tomura, RC Hovey, J Sugawara, H Yoneyama, H Kitazawa, K Watanabe, H Aso, T Nochi
    Cell Reports, 2021-09-07;36(10):109655.
    Species: Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. CCL28 promotes locomotor recovery after spinal cord injury via recruiting regulatory T cells
    Authors: P Wang, X Qi, G Xu, J Liu, J Guo, X Li, X Ma, H Sun
    Aging (Albany NY), 2019-09-26;11(18):7402-7415.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Expression of CD11c Is Associated with Unconventional Activated T Cell Subsets with High Migratory Potential
    PLoS ONE, 2016-04-27;11(4):e0154253.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.
    Authors: Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G
    Nature, 2011-07-13;475(7355):226-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Regulation of humoral and cellular gut immunity by lamina propria dendritic cells expressing Toll-like receptor 5.
    Authors: Uematsu S, Fujimoto K, Jang MH, Yang BG, Jung YJ, Nishiyama M, Sato S, Tsujimura T, Yamamoto M, Yokota Y, Kiyono H, Miyasaka M, Ishii KJ, Akira S
    Nat. Immunol., 2008-05-30;9(7):769-76.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Mouse/Rat CCR10 APC-conjugated Antibody

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Mouse/Rat CCR10 APC‑conjugated Antibody
By Anonymous on 10/26/2015
Application: Flow Sample Tested: activated mouse CD8 T cell Species: Mouse

Cells were stained for 10 minutes in room temperature followed by three washes. Isotype control was done in parallel.
Specificity: Reasonably specific
Sensitivity: Not sensitive
Buffer: PBS + 2.5% FBS
Dilution: 1/10

Flow Cytometry Mouse/Rat CCR10 APC‑conjugated Antibody FAB2815A