Mouse/Rat DARC APC-conjugated Antibody Summary
Met1-Pro61, Ala115-Cys127, Ser186-Lys205, Tyr264-Asn285
Accession # NP_034175
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of DARC in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse TER-119 PE-conjugated Monoclonal Antibody (Catalog # FAB1125P) and either (A) Sheep Anti-Mouse/Rat DARC APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6695A) or (B) Normal Sheep IgG Allophycocyanin Control (Catalog # IC016A). View our protocol for Staining Membrane-associated Proteins.
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Detection of DARC in HEK293 Human Cell Line Transfected with Mouse DARC and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse DARC and eGFP was stained with either (A) Sheep Anti-Mouse/Rat DARC APC-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB6695A) or (B) Normal Sheep IgG Allophycocyanin Control (Catalog # IC016A). View our protocol for Staining Membrane-associated Proteins.
Reconstitution Calculator
Preparation and Storage
Background: DARC
DARC (Duffy Antigen Receptor for Chemokines; also CD234) is a 40-46 kDa glycoprotein member of the Duffy family of silent heptahelical chemokine receptors. It is expressed in liver and on select neurons, erythrocytes and the endothelium of postcapillary venules. Unlike traditional chemokine receptors, DARC cannot signal through G-proteins as it lacks a DRYLAIVHA cytoplasmic motif. DARC has three potential functions: first, it binds circulating inflammatory-type chemokines, serving as a repository for future chemokine release; second, it acts as a vehicle by which chemokines are transported from the abluminal to the luminal side of endothelium; and third, it complexes with signal-transducing chemokine receptors to create a nonsignaling heterodimer. Mouse DARC is 334 amino acids (aa) in length. It contains a 62 aa N-terminal extracellular region, and a 28 aa C-terminal cytoplasmic tail. There is one potential splice variant that shows a 42 aa substitution for aa 133-334. Collectively, over the four extracellular domains (aa 1-62, 115-127, 186-205, 264-285), mouse DARC shares 52% and 75% aa identity with human and rat DARC, respectively.
Product Datasheets
Citation for Mouse/Rat DARC APC-conjugated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Interleukin-22 Induces the Infiltration of Visceral Fat Tissue by a Discrete Subset of Duffy Antigen Receptor for Chemokine-Positive M2-Like Macrophages in Response to a High Fat Diet
Authors: EY Kim, HM Noh, B Choi, JE Park, JE Kim, Y Jang, HK Lee, EJ Chang
Cells, 2019-12-06;8(12):.
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