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Mouse/Rat DARPP-32 Antibody

Catalog #: MAB4230
15 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB4230
MAB4230-SP
Detection of Mouse/Rat DARPP‑32 by Western Blot.
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Citations (15)
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Mouse/Rat DARPP-32 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat DARPP-32. Reactivity with human DARPP-32 has not been tested.
Source
Monoclonal Rat IgG2A Clone # 375604
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human DARPP-32
Ala51-Thr204
Accession # Q9UD71.2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
Perfusion fixed paraffin-embedded sections of rat brain (corpus striatum)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse/Rat DARPP‑32 antibody by Western Blot. View Larger

Detection of Mouse/Rat DARPP‑32 by Western Blot. Western blot shows lysates of mouse brain corpus striatum tissue. PVDF membrane was probed with 1 µg/mL of Mouse/Rat DARPP-32 Monoclonal Antibody (Catalog # MAB4230) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for DARPP-32 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DARPP-32

Dopamine- and cAMP-Regulated Phosphoprotein, Mr 32 kDa (DARPP-32), also known as PPP1R1B, is a 23 kilodalton protein that anomalously migrates at about
32‑35 kDa on SDS-PAGE. When phosphorylated at T34 by protein kinase A (PKA), DARPP-32 is a potent inhibitor of protein phosphatase 1 (PP1). Dephosphorylation of DARPP-32 at T34 is achieved primarily by the calcium-dependent activation of the phosphatase calcineurin. DARPP-32 is expressed almost exclusively in neuronal tissues, with highest levels in dopamine-innervated neurons.

Long Name
Dopamine and cAMP-regulated Phosphoprotein
Entrez Gene IDs
84152 (Human); 19049 (Mouse); 360616 (Rat)
Alternate Names
DARPP32; DARPP-32; PPP1R1B; protein phosphatase 1, regulatory (inhibitor) subunit 1B; regulatory (inhibitor) subunit 1B (dopamine and cAMPregulated phosphoprotein, DARPP-32)

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Citations for Mouse/Rat DARPP-32 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. Modulation of miR-181 influences dopaminergic neuronal degeneration in a mouse model of Parkinson’s disease
    Authors: Colleen S. Stein, Jared M. McLendon, Nathan H. Witmer, Ryan L. Boudreau
    Molecular Therapy - Nucleic Acids
  2. CD40L Reverse Signaling Influences Dendrite Spine Morphology and Expression of PSD-95 and Rho Small GTPases
    Authors: Paulina Carriba, Sean Wyatt, Alun M. Davies
    Frontiers in Cell and Developmental Biology
  3. Circuit-Selective Striatal Synaptic Dysfunction in the Sapap3 Knockout Mouse Model of Obsessive-Compulsive Disorder
    Authors: Yehong Wan, Kristen K. Ade, Zachary Caffall, M. Ilcim Ilcim Ozlu, Cagla Eroglu, Guoping Feng et al.
    Biological Psychiatry
  4. Tumor suppressor p53 regulates heat shock factor 1 protein degradation in Huntington's disease
    Authors: Mansky RH, Greguske EA, Yu D et al.
    Cell reports
  5. Cellular and subcellular localization of Rab10 and phospho-T73 Rab10 in the mouse and human brain
    Authors: Singh, V;Menard, MA;Serrano, GE;Beach, TG;Zhao, HT;Riley-DiPaolo, A;Subrahmanian, N;LaVoie, MJ;Volpicelli-Daley, LA;
    Acta neuropathologica communications
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  6. Regional vulnerability in a neurodegenerative disease: Delineating SCA1 CNS and muscle therapeutic targets using a conditional mutant ATXN1 mouse
    Authors: L Duvick, WM Southern, K Benzow, HP Handler, JS Mitchell, H Kuivinen, UK Gadiparthi, P Yang, A Soles, C Scheeler, O Rainwater, S Shannah, E Larson, T Nichols-Me, Y You, B Oâ Callagh, HY Zoghbi, JM Ervasti, M Cvetanovic, MD Koob, HT Orr
    bioRxiv : the preprint server for biology, 2023-04-11;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  7. Short antisense oligonucleotides alleviate the pleiotropic toxicity of RNA harboring expanded CGG repeats
    Authors: M Derbis, E Kul, D Niewiadoms, M Sekrecki, A Piasecka, K Taylor, RK Hukema, O Stork, K Sobczak
    Nature Communications, 2021-02-24;12(1):1265.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. DAPK1 Promotes Extrasynaptic GluN2B Phosphorylation and Striatal Spine Instability in the YAC128 Mouse Model of Huntington Disease
    Authors: ME Schmidt, NS Caron, AE Aly, FL Lemarié, L Dal Cengio, Y Ko, N Lazic, L Anderson, B Nguyen, LA Raymond, MR Hayden
    Front Cell Neurosci, 2020-11-05;14(0):590569.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  9. Striatal Projection Neurons Require Huntingtin for Synaptic Connectivity and Survival
    Authors: CJ Burrus, SU McKinstry, N Kim, MI Ozlu, AV Santoki, FY Fang, A Ma, YB Karadeniz, AK Worthingto, I Dragatsis, S Zeitlin, HH Yin, C Eroglu
    Cell Rep, 2020-01-21;30(3):642-657.e6.
    Species: Mouse
    Sample Types: Tissue
    Applications: IHC
  10. Potent and sustained huntingtin lowering via AAV5 encoding miRNA preserves striatal volume and cognitive function in a humanized mouse model of Huntington disease
    Authors: NS Caron, AL Southwell, CC Brouwers, LD Cengio, Y Xie, HF Black, LM Anderson, S Ko, X Zhu, SJ van Devent, MM Evers, P Konstantin, MR Hayden
    Nucleic Acids Res., 2020-01-10;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Altering cortical input unmasks synaptic phenotypes in the YAC128 cortico-striatal co-culture model of Huntington disease
    Authors: ME Schmidt, C Buren, JP Mackay, D Cheung, L Dal Cengio, LA Raymond, MR Hayden
    BMC Biol., 2018-06-27;16(1):58.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  12. Abnormal degradation of the neuronal stress-protective transcription factor HSF1 in Huntington's disease
    Authors: R Gomez-Past, ET Burchfiel, DW Neef, AM Jaeger, E Cabiscol, SU McKinstry, A Doss, A Aballay, DC Lo, SS Akimov, CA Ross, C Eroglu, DJ Thiele
    Nat Commun, 2017-02-13;8(0):14405.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Sapap3 deletion anomalously activates short-term endocannabinoid-mediated synaptic plasticity.
    Authors: Chen, Meng, Wan, Yehong, Ade, Kristen, Ting, Jonathan, Feng, Guoping, Calakos, Nicole
    J Neurosci, 2011-06-29;31(26):9563-73.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: IHC
  14. Early life exposure to a high fat diet promotes long-term changes in dietary preferences and central reward signaling.
    Authors: Teegarden SL, Scott AN, Bale TL
    Neuroscience, 2009-05-22;162(4):924-32.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  15. The interaction of aging and oxidative stress contributes to pathogenesis in mouse and human Huntington disease neurons
    Authors: Machiela E, Jeloka R, Caron NS et al.
    Front Aging Neurosci

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