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Mouse/Rat GABAB R2 Antibody

Catalog #: MAB104561 Datasheet / COA / SDS
Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB104561-100
MAB104561-SP
Detection of Mouse and Rat GABAB R2 by Western Blot.
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Mouse/Rat GABAB R2 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects rat GABAB R2 in direct ELISAs. Detects mouse and rat GABAB R2 in Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2605F
Purification
Protein A or G purified from egg yolks
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat GABAB R2
Trp41-Ser482
Accession # O88871
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Mouse brain tissue and Rat brain tissue
Simple Western
20 µg/mL
Rat brain tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse and Rat GABA<sub>B</sub> R2 antibody by Western Blot. View Larger

Detection of Mouse and Rat GABAB R2 by Western Blot. Western blot shows lysates of mouse brain tissue and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Mouse/Rat GABAB R2 Monoclonal Antibody (Catalog # MAB104561) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for GABAB R2 at approximately 116 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western Detection of Rat GABA<sub>B</sub> R2 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Rat GABAB R2 by Simple WesternTM. Simple Western lane view shows lysates of rat brain tissue, loaded at 0,.2 mg/mL. A specific band was detected for GABAB R2 at approximately 148 kDa (as indicated) using 20 µg/mL of Rabbit Anti-Mouse/Rat GABAB R2 Monoclonal Antibody (Catalog # MAB104561). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GABA-B R2

GABBR2 is half of a heterodimeric GPCR for GABA which mediates coupling to G proteins. B-type receptors for the neurotransmitter GABA (gamma-aminobutyric acid) inhibit neuronal activity through G protein-coupled second-messenger systems, which regulate the release of neurotransmitters and the activity of ion channels and adenylyl cyclase As a homodimer, GABBR2 is retained in the endoplasmic reticulum and endoplasmic reticulum-Golgi intermediate compartment.  When GBR1/GBR2 heterodimers are present they are localized to the plasma membrane. GABBR2 is differentially expressed in the nervous system. GABBR2 and GABBR1 mRNA's are coexpressed in various brain regions such as the Purkinje cell layer of the cerebellum. In situ hybridization histochemistry using an antisensce probe to this novel receptor indicates GABBR2 is found exclusively in neurons. GABBR2 is implicated in synaptic inhibition, hippocampal long-term potentiation, slow wave sleep, muscle relaxation and nociception.

Long Name
gamma-Aminobutyric Acid Type B Receptor Subunit 2
Entrez Gene IDs
9568 (Human); 83633 (Rat)
Alternate Names
G protein-coupled receptor 51; GABA B R2; GABABR2; GABA-BR2; GABA-B-R2; GABABR2GABA-B receptor 2; GABBR2; gamma-aminobutyric acid (GABA) B receptor, 2; gamma-aminobutyric acid type B receptor subunit 2; gb2; GPR51; GPR51GABA-B receptor, R2 subunit; GPRC3B; GPRC3BFLJ36928; G-protein coupled receptor 51; HG20HRIHFB2099

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