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Mouse/Rat HO-1/HMOX1/HSP32 Antibody

Catalog #: AF3169
4 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3169
AF3169-SP
Detection of Mouse/Rat HO‑1/HMOX1/HSP32 by Western Blot.
2 Images
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat HO-1/HMOX1/HSP32 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat HO-1/HMOX1/HSP32 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant rat HO-1/HMOX1
Met1-Met289
Accession # P06762
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse/Rat HO‑1/HMOX1/HSP32 antibody by Western Blot. View Larger

Detection of Mouse/Rat HO‑1/HMOX1/HSP32 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, A20 mouse B cell lymphoma cell line, NRK rat normal kidney cells, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse/Rat HO‑1/HMOX1/HSP32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3169) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HO-1/HMOX1/HSP32 at approximately 32 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.

Western Blot Detection of Rat HO-1/HMOX1/HSP32 by Western Blot View Larger

Detection of Rat HO-1/HMOX1/HSP32 by Western Blot Effect of prodigiosins on the mRNA and protein expression of HO-1 in gastric tissue.Data (means ± SEM of three assays) were normalized to the levels of GAPDH and expressed as fold induction (log2 scale), relative to the level of HO-1 in the control. aP < 0.05, significant difference vs. the health rats; bP < 0.05, significant difference vs. HCl/ethanol-induced rats, Duncan's post-hoc test. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31194753), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HO-1/HMOX1/HSP32

Heme Oxygenase 1 (HO-1), also known as HMOX1 and Heat Shock Protein 32 (HSP32), is a 32 kDa microsomal enzyme required for the metabolism of heme to biliverdin. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 (HO-1/HMOX1) and a constitutive heme oxygenase-2 (HO-2/HMOX2). HO-1 expression is induced by heme and other non-heme compounds. Rat HO-1 shares 93% amino acid sequence identity with mouse HO-1.

Long Name
Heme Oxygenase 1
Entrez Gene IDs
3162 (Human); 24451 (Mouse); 15368 (Rat)
Alternate Names
bK286B10; EC 1.14.99.3; heat shock protein, 32-kD; heme oxygenase (decycling) 1; HMOX1; HO; HO1; HO-1; HO-1heme oxygenase 1; HSP32

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Citations for Mouse/Rat HO-1/HMOX1/HSP32 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Prodigiosins from a marine sponge-associated actinomycete attenuate HCl/ethanol-induced gastric lesion via antioxidant and anti-inflammatory mechanisms
    Authors: MS Abdelfatta, MIY Elmallah, HY Ebrahim, RS Almeer, RMA Eltanany, AE Abdel Mone
    PLoS ONE, 2019-06-13;14(6):e0216737.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  2. Royal jelly attenuates cadmium-induced nephrotoxicity in male mice
    Authors: RS Almeer, GI AlBasher, S Alarifi, S Alkahtani, D Ali, AE Abdel Mone
    Sci Rep, 2019-04-09;9(1):5825.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Paeoniflorin Attenuated Oxidative Stress in Rat COPD Model Induced by Cigarette Smoke
    Evid Based Complement Alternat Med, 2016-11-28;2016(0):1698379.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Genipin protects against H2O2-induced oxidative damage in�retinal pigment epithelial cells by promoting Nrf2 signaling
    Authors: Hailan Zhao, Ruiqing Wang, Mingxia Ye, Lan Zhang
    International Journal of Molecular Medicine

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Reviews for Mouse/Rat HO-1/HMOX1/HSP32 Antibody

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Mouse/Rat HO-1/HMOX1/HSP32 Antibody
By Anonymous on 06/01/2016
Application: WB Sample Tested: Colon tissue,Kidney tissue,Liver tissue,mouse embryonic fibroblasts,Small intestine tissue Species: Human, Mouse, Rat

Primary ab dilution 1:2000 (better with 1:1000)
40 ug protein per lane

Western Blot Mouse/Rat HO-1/HMOX1/HSP32 Antibody AF3169