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Mouse/Rat IL-10 Antibody

Catalog #: AF519
33 Citations 5 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF519
AF519-SP
Detection of Recombinant Mouse and Rat IL‑10 by Western Blot.
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Product Details
Citations (33)
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Reviews (5)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat IL-10 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse, rat, and cotton rat IL-10 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human IL-10, recombinant guinea pig IL-10, recombinant viral IL-10 and recombinant canine IL-10 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant rat IL‑10
Ser19-Asn178
Accession # P29456
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
Immersion fixed mouse splenocytes
Neutralization
Measured by its ability to neutralize IL‑10-induced proliferation in the MC/9‑2 mouse mast cell line. Thompson-Snipes, L.  et al. (1991) J. Exp. Med. 173:507. The Neutralization Dose (ND50) is typically 0.3-1.0 µg/mL in the presence of 40 ng/mL Recombinant Rat IL‑10.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Recombinant Mouse and Rat IL-10 antibody by Western Blot. View Larger

Detection of Recombinant Mouse and Rat IL‑10 by Western Blot. Western blot shows 25 ng of Recombinant Mouse IL-10 (Catalog # 417-ML), Recombinant Human IL-10 (Catalog # 217-IL), Recombinant Rat IL-10 (Catalog # 522-RLB), and Recombinant Human IL-26/AK155 Monomer (Catalog # 1375-IL). PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Mouse/Rat IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF519) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunocytochemistry View Larger

IL‑10 in Mouse Splenocytes. IL‑10 was detected in immersion fixed mouse splenocytes using Goat Anti-Mouse/Rat IL‑10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF519) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Neutralization Cell Proliferation Induced by IL‑10 and Neutralization by Rat IL‑10 Antibody. View Larger

Cell Proliferation Induced by IL‑10 and Neutralization by Rat IL‑10 Antibody. Recombinant Rat IL-10 (Catalog # 522-RL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-10 (40 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF519). The ND50 is typically 0.3-1.0 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-10

Interleukin 10, also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of alpha -helical cytokines that also includes IL-19, IL‑20, IL‑22, and IL-24 (1, 2). IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts (2‑5). Mature rat IL‑10 shares 85% amino acid sequence identity with mouse and 71%‑79% amino acid sequence identity with bovine, canine, equine, feline, human, ovine, and porcine IL-10. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells (6, 7). IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer (8‑10). The IL-10 dimer binds to two IL‑10 R alpha /IL‑10 R1 chains, resulting in recruitment of two IL-10 R beta /IL-10R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3 (11). IL‑10 R beta does not bind IL-10 by itself but is required for signal transduction (1). IL-10 R beta also associates with IL‑20 R alpha, IL-22 R alpha, or IL-28 R alpha to form the receptor complexes for IL-22, IL-26, IL-28, and IL-29 (12‑14). IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation (15‑17). It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses (2).

References
  1. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.
  2. O’Garra, A. and P. Vieira (2007) Nat. Rev. Immunol. 7:425.
  3. Mathurin, P. et al. (2002) Am. J. Physiol. Gastrointest. Liver Physiol. 282:G981.
  4. Grewe, M. et al. (1995) J. Invest. Dermatol. 104:3.
  5. Szony, B.J. et al. (1999) Mol. Hum. Reprod. 5:1059.
  6. Vieira, P. et al. (1991) Proc. Natl. Acad. Sci. 88:1172.
  7. Hsu, D.-H. et al. (1990) Science 250:830.
  8. Goodman, R. et al. (1992) Biochem. Biophys. Res. Commun. 189:1.
  9. Windsor, W.T. et al. (1993) Biochemistry 32:8807.
  10. Syto, R. et al. (1998) Biochemistry 37:16943.
  11. Kotenko, S.V. et al. (1997) EMBO J. 16:5894.
  12. Kotenko, S.V. et al. (2000) J. Biol. Chem. 276:2725.
  13. Hor, S. et al. (2004) J. Biol. Chem. 279:33343.
  14. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
  15. Fitzgerald, D.C. et al. (2007) Nat. Immunol. 8:1372.
  16. Wu, K. et al. (2007) Cell. Mol. Immunol. 4:269.
  17. Blackburn, S.D. and E.J. Wherry (2007) Trends Microbiol. 15:143.
Long Name
Interleukin 10
Entrez Gene IDs
3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 102133450 (Cynomolgus Monkey); 493683 (Feline); 100715618 (Guinea Pig); 2949786 (Viral)
Alternate Names
CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF

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Citations for Mouse/Rat IL-10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

33 Citations: Showing 1 - 10
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  1. Bio-inspired poly-DL-serine materials resist the foreign-body response
    Authors: D Zhang, Q Chen, Y Bi, H Zhang, M Chen, J Wan, C Shi, W Zhang, J Zhang, Z Qiao, J Li, S Chen, R Liu
    Nature Communications, 2021-09-07;12(1):5327.
  2. Peripheral versus central mechanisms of the cannabinoid type 2 receptor agonist AM1710 in a mouse model of neuropathic pain
    Authors: Wilkerson JL, Alberti LB, Kerwin AA et al.
    Brain Behav
  3. Adenine Nucleotide Translocase 1 Expression Modulates the Immune Response in Ischemic Hearts
    Authors: F Yergöz, J Friebel, N Kränkel, U Rauch-Kroe, HP Schultheis, U Landmesser, A Dörner
    Cells, 2021-08-19;10(8):.
  4. Prokineticin-2 prevents neuronal cell deaths in a model of traumatic brain injury
    Authors: Bao Z, Liu Y, Chen B Et Al.
    Nature communications
  5. Minus end-directed kinesin-14 KIFC1 regulates the positioning and architecture of the Golgi apparatus.
    Authors: She ZY, Pan MY, Tan FQ, Yang, WX.
    Oncotarget.
  6. LncEDCH1 improves mitochondrial function to reduce muscle atrophy by interacting with SERCA2
    Authors: Cai B, Ma M, Zhang J et al.
    Molecular Therapy - Nucleic Acids
  7. Mitophagy inhibits amyloid-b and tau pathology and reverses cognitive deficits in models of Alzheimer's disease
    Authors: Fang EF, Hou Y, Palikaras K et al.
    Nat. Neurosci.
  8. Galectin-3 as TREM2 upstream factor contributes to lung ischemia-reperfusion injury by regulating macrophage polarization
    Authors: Liu H, Zhang L, Liu Z et al.
    iScience
  9. Essential roles of the cytokine oncostatin M in crosstalk between muscle fibers and immune cells in skeletal muscle after aerobic exercise
    Authors: T Komori, Y Morikawa
    The Journal of Biological Chemistry, 2022-11-09;0(0):102686.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. Cellular Sources and Neuroprotective Roles of Interleukin-10 in the Facial Motor Nucleus after Axotomy
    Authors: EM Runge, DO Setter, AK Iyer, EJ Regele, FM Kennedy, VM Sanders, KJ Jones
    Cells, 2022-10-09;11(19):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Ginsenoside RG1 enhances the paracrine effects of bone marrow-derived mesenchymal stem cells on radiation induced intestinal injury
    Authors: Yujun Luo, Beibei Wang, Jianhua Liu, Faxin Ma, Dongling Luo, Zhongwen Zheng et al.
    Aging (Albany NY)
  12. Inhibition of Serine Protease Activity Protects Against High Fat Diet-Induced Inflammation and Insulin Resistance
    Authors: CS Kuo, JS Chen, LY Lin, GW Schmid-Sch, S Chien, PH Huang, JW Chen, SJ Lin
    Sci Rep, 2020-02-03;10(1):1725.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  13. Involvement of Interleukin-10 in Analgesia of Electroacupuncture on Incision Pain
    Authors: WJ Dai, JL Sun, C Li, W Mao, YK Huang, ZQ Zhao, YQ Zhang, N Lü
    Evid Based Complement Alternat Med, 2019-10-30;2019(0):8413576.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-Fr, Western Blot
  14. Human mesenchymal stromal cells ameliorate complement induced inflammatory cascade and improve renal functions in a rat model of ischemia-reperfusion induced acute kidney injury
    Authors: S Zilberman-, R Abu-Hamad, R Zarura, M Sova, Y Hachmo, M Stark, S Neuman, S Slavin, S Efrati
    PLoS ONE, 2019-09-12;14(9):e0222354.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: Fluorescence Microscopy
  15. Activation of GPR40 produces mechanical antiallodynia via the spinal glial interleukin-10/?-endorphin pathway
    Authors: XF Mao, HY Wu, XQ Tang, U Ali, H Liu, YX Wang
    J Neuroinflammation, 2019-04-13;16(1):84.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  16. PD-1/PD-L1 checkpoint blockade harnesses monocyte-derived macrophages to combat cognitive impairment in a tauopathy mouse model
    Authors: Neta Rosenzweig, Raz Dvir-Szternfeld, Afroditi Tsitsou-Kampeli, Hadas Keren-Shaul, Hila Ben-Yehuda, Pierre Weill-Raynal et al.
    Nature Communications
  17. Blocking p38 Signaling Reduces the Activation of Pro-inflammatory Cytokines and the Phosphorylation of p38 in the Habenula and Reverses Depressive-Like Behaviors Induced by Neuroinflammation
    Authors: YW Zhao, YQ Pan, MM Tang, WJ Lin
    Front Pharmacol, 2018-05-15;9(0):511.
    Species: Rat
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  18. Autocrine interleukin-10 mediates glucagon-like peptide-1 receptor-induced spinal microglial ?-endorphin expression
    Authors: HY Wu, XQ Tang, XF Mao, YX Wang
    J. Neurosci., 2017-10-30;0(0):.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  19. A neuropeptide, Substance-P, directly induces tissue-repairing M2 like macrophages by activating the PI3K/Akt/mTOR pathway even in the presence of IFN?
    Authors: JE Lim, E Chung, Y Son
    Sci Rep, 2017-08-25;7(1):9417.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  20. ST2/IL-33-Dependent Microglial Response Limits Acute Ischemic Brain Injury
    Authors: Y Yang, H Liu, H Zhang, Q Ye, J Wang, B Yang, L Mao, W Zhu, RK Leak, B Xiao, B Lu, J Chen, X Hu
    J. Neurosci., 2017-04-07;0(0):.
    Species: Mouse, Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  21. Cooperation of Gastric Mononuclear Phagocytes with Helicobacter pylori during Colonization
    Authors: M Viladomiu, J Bassaganya, N Tubau-Juni, B Kronsteine, A Leber, CW Philipson, V Zoccoli-Ro, R Hontecilla
    J. Immunol, 2017-03-06;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  22. Y RNA fragment in extracellular vesicles confers cardioprotection via modulation of IL-10 expression and secretion
    Authors: L Cambier, G de Couto, A Ibrahim, AK Echavez, J Valle, W Liu, M Kreke, RR Smith, L Marbán, E Marbán
    EMBO Mol Med, 2017-03-01;9(3):337-352.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  23. Breaking immune tolerance by targeting Foxp3(+) regulatory T cells mitigates Alzheimer's disease pathology
    Authors: Kuti Baruch, Neta Rosenzweig, Alexander Kertser, Aleksandra Deczkowska, Alaa Mohammad Sharif, Amit Spinrad et al.
    Nature Communications
  24. Therapeutic effects of glatiramer acetate and grafted CD115⁺ monocytes in a mouse model of Alzheimer's disease
    Authors: Yosef Koronyo, Brenda C. Salumbides, Julia Sheyn, Lindsey Pelissier, Songlin Li, Vladimir Ljubimov et al.
    Brain
  25. Immunization with a Myelin-Derived Antigen Activates the Brain's Choroid Plexus for Recruitment of Immunoregulatory Cells to the CNS and Attenuates Disease Progression in a Mouse Model of ALS.
    Authors: Kunis G, Baruch K, Miller O, Schwartz M
    J Neurosci, 2015-04-22;35(16):6381-93.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  26. Cerebral regulatory T cells restrain microglia/macrophage-mediated inflammatory responses via IL-10.
    Authors: Xie L, Choudhury G, Winters A, Yang S, Jin K
    Eur J Immunol, 2014-11-24;45(1):180-91.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neutralization
  27. CNS repair requires both effector and regulatory T cells with distinct temporal and spatial profiles.
    Authors: Raposo C, Graubardt N, Cohen M, Eitan C, London A, Berkutzki T, Schwartz M
    J Neurosci, 2014-07-30;34(31):10141-55.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  28. Decrease in IL-10 and increase in TNF-alpha levels in renal tissues during systemic inhibition of nitric oxide in anesthetized mice
    Authors: Purnima Singh, Alexander Castillo, Dewan S. A. Majid
    Physiological Reports
  29. Serum-starved adipose-derived stromal cells ameliorate crescentic GN by promoting immunoregulatory macrophages.
    Authors: Furuhashi, Kazuhiro, Tsuboi, Naotake, Shimizu, Asuka, Katsuno, Takayuki, Kim, Hangsoo, Saka, Yosuke, Ozaki, Takenori, Sado, Yoshikaz, Imai, Enyu, Matsuo, Seiichi, Maruyama, Shoichi
    J Am Soc Nephrol, 2013-03-07;24(4):587-603.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  30. Immunofluorescent spectral analysis reveals the intrathecal cannabinoid agonist, AM1241, produces spinal anti‐inflammatory cytokine responses in neuropathic rats exhibiting relief from allodynia
    Authors: Jenny L. Wilkerson, Katherine R. Gentry, Ellen C. Dengler, James A. Wallace, Audra A. Kerwin, Megan N. Kuhn et al.
    Brain and Behavior
  31. Functionalized scaffold-mediated interleukin 10 gene delivery significantly improves survival rates of stem cells in vivo.
    Authors: Holladay C, Power K, Sefton M, O'Brien T, Gallagher WM, Pandit A
    Mol. Ther., 2011-01-25;19(5):969-78.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  32. Ocular regulatory T cells distinguish monophasic from recurrent autoimmune uveitis.
    Authors: Ke Y, Jiang G, Sun D, Kaplan HJ, Shao H
    Invest. Ophthalmol. Vis. Sci., 2008-05-16;49(9):3999-4007.
    Species: Rat
    Sample Types: Aqueous Humor
    Applications: Neutralization
  33. Oxo-M and 4-PPBP Delivery via Multi-Domain Peptide Hydrogel Toward Tendon Regeneration
    Authors: Park GY, Tarafder S, Eyen SL et al.
    Frontiers in bioengineering and biotechnology

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Reviews for Mouse/Rat IL-10 Antibody

Average Rating: 4.6 (Based on 5 Reviews)

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Mouse/Rat IL-10 Antibody
By Anonymous on 08/28/2022
Application: Flow Sample Tested: Myeloid cells Species: Mouse

Try this for flow, the signal is not too strong, may need optimize.

Flow Cytometry Mouse/Rat IL-10 Antibody AF519
Mouse/Rat IL-10 Antibody
By Alex O'Neal on 11/30/2020
Application: WB Sample Tested: Bronchoalveolar lavage Species: Rat

We had an issue with shipping due to COVID, that caused the antibody to sit at room temperature for too long and become unusable. Fortunately we placed another order of the exact same product number, and it performed perfectly. I've included the photo of the gel after the shipping mishap

Western Blot Mouse/Rat IL-10 Antibody AF519
Mouse/Rat IL-10 Antibody
By Alex O'Neal on 11/30/2020
Application: WB Sample Tested: Control Species: Rat

This image was purely a test with an IL-10 control protein at different concentrations. Initially we were unable to detect the IL-10 protein in our samples, so we were testing to ensure that this antibody was functional. Turns out it was!

Western Blot Mouse/Rat IL-10 Antibody AF519
Mouse/Rat IL-10 Antibody
By Danlee Enzler on 04/24/2018
Application: IHC Sample Tested: Spleen tissue Species: Mouse
Mouse/Rat IL-10 Antibody
By Anonymous on 07/27/2016
Application: B/N Sample Tested: Splenic B cells Species: Mouse