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Mouse & Rat IL-17F ELISA Kit - Quantikine

Catalog #: M17F0
5 Citations Datasheet / COA / SDS
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M17F0
Mouse/Rat IL-17F ELISA Standard Curve
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Citations (5)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Mouse/Rat IL-17F Quantikine ELISA Kit Summary

Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)
Sensitivity
1.74 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant mouse and rat IL-17F.
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse/Rat IL-17F Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse and rat IL-17F in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-17F and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse or rat IL-17F showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse and rat IL-17F.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 56.7 211 409 53.2 202 399
Standard Deviation 1.26 4.93 13.1 3.98 11.4 20.2
CV% 2.2 2.3 3.2 7.5 5.6 5.1

Recovery

The recovery of mouse IL-17F spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 96 92-99
EDTA Plasma (n=4) 107 101-118
Heparin Plasma (n=4) 101 88-121
Serum (n=4) 95 83-109

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of mouse IL-17F in each matrix were diluted with Calibrator Diluent and assayed.
Mouse/Rat IL-17F ELISA Linearity

Scientific Data

N/A Mouse/Rat IL-17F ELISA Standard Curve View Larger

Mouse/Rat IL-17F ELISA Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-17F

The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.

Long Name:
Interleukin 17F
Entrez Gene IDs:
112744 (Human); 257630 (Mouse); 301291 (Rat); 708220 (Rhesus Macaque)
Alternate Names:
Cytokine ML-1; IL17F; IL-17F; IL-17Finterleukin-17F; interleukin 17F
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Mouse/Rat IL-17F Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Study on the effect and mechanism of quercetin in treating gout arthritis
    Authors: W Feng, XQ Zhong, XX Zheng, QP Liu, MY Liu, XB Liu, CS Lin, Q Xu
    International immunopharmacology, 2022-08-03;111(0):109112.
    Species: Rat
    Sample Types: Serum
  2. Interleukine-17 Modulates Neurogenesis and Behavior Following Exposure to Trauma in Mice
    Authors: Y Willinger, G Turgeman
    Cells, 2022-01-20;11(3):.
    Species: Mouse
    Sample Types: Serum
  3. Non-alcoholic fatty liver disease is associated with bacterial translocation and a higher inflammation response in psoriatic patients
    Authors: I Belinchón-, P Bellot, D Romero-Pér, I Herraiz-Ro, F Marco, R Frances, JM Ramos-Rinc
    Scientific Reports, 2021-04-21;11(1):8593.
    Species: Rat
    Sample Types: Serum
  4. Long non-coding RNA ANRIL knockdown suppresses apoptosis and pro-inflammatory cytokines while enhancing neurite outgrowth via binding microRNA-125a in a cellular model of Alzheimer's disease
    Authors: B Zhou, L Li, X Qiu, J Wu, L Xu, W Shao
    Mol Med Rep, 2020-06-02;22(2):1489-1497.
    Species: Rat
    Sample Types: Cell Culture Supernates
  5. Interleukin-17A is involved in mechanical hyperalgesia but not in the severity of murine antigen-induced arthritis
    Authors: M Ebbinghaus, G Natura, G Segond von, S Hensellek, M Böttcher, B Hoffmann, FS Salah, M Gajda, T Kamradt, HG Schaible
    Sci Rep, 2017-09-04;7(1):10334.
    Species: Mouse
    Sample Types: Cell Culture Supernates

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