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Mouse/Rat MIS/AMH Antibody

Catalog #: AF1446
4 Citations Datasheet / COA / SDS
Newer Version Available: MAB1446
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Discontinued Product

AF1446 has been discontinued and is replaced by MAB1426. MAB1446.

Product Details
Citations (4)
FAQs
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat MIS/AMH Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat MIS/AMH in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross‑reactivity with recombinant human MIS is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant rat MIS/AMH
Lys447-Arg553
Accession # P49000
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse MIS/AMH
Recombinant Rat MIS/AMH
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse ovary

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MIS/AMH

MIS, also known as anti-Müllarian hormone (AMH), belongs to the TGF-beta superfamily. It is produced by the fetal testis and plays a central role in male sex differentiation. Postnatally, MIS is expressed in both the testis and the ovary, where it also has a regulatory role in gonadal functions.

Long Name
Mullerian-inhibiting Substance/Anti-Mullerian Hormone
Entrez Gene IDs
268 (Human); 11705 (Mouse)
Alternate Names
AMH; MIF; MIS; Muellerian hormone; muellerian-inhibiting factor; muellerian-inhibiting substance; Mullerian hormone; Mullerian inhibiting factor; Mullerian inhibiting substance

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Citations for Mouse/Rat MIS/AMH Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. The gene encoding the ketogenic enzyme HMGCS2 displays a unique expression during gonad development in mice
    Authors: S Bagheri-Fa, H Chen, S Wilson, K Ayers, J Hughes, F Sloan-Bena, P Calvel, G Robevska, B Puisac, K Kusz-Zamel, S Gimelli, A Spik, J Jaruzelska, A Warenik-Sz, S Faradz, S Nef, J Pié, P Thomas, A Sinclair, D Wilhelm
    PLoS ONE, 2020-01-07;15(1):e0227411.
    Species: Mouse
    Sample Types: Tissue
    Applications: IF
  2. Long-Term Moderate Oxidative Stress Decreased Ovarian Reproductive Function by Reducing Follicle Quality and Progesterone Production
    PLoS ONE, 2016-09-27;11(9):e0162194.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Inhibitory actions of Anti-Mullerian Hormone (AMH) on ovarian primordial follicle assembly.
    Authors: Nilsson EE, Schindler R, Savenkova MI, Skinner MK
    PLoS ONE, 2011-05-27;6(5):e20087.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Mullerian inhibiting substance contributes to sex-linked biases in the brain and behavior.
    Authors: Wang PY, Protheroe A, Clarkson AN, Imhoff F, Koishi K, McLennan IS
    Proc. Natl. Acad. Sci. U.S.A., 2009-04-09;106(17):7203-8.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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