Home / Neuropilin-2 / Mouse/Rat Neuropilin-2 Antibody

Mouse/Rat Neuropilin-2 Antibody

Catalog #: AF567
39 Citations 3 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF567
AF567-SP
Detection of Mouse and Rat Neuropilin‑2 by Western Blot.
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Product Details
Citations (39)
FAQs
Supplemental Products
Reviews (3)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat Neuropilin-2 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat Neuropilin-2 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross‑reactivity with recombinant human Neuropilin-2 and recombinant rat Neuropilin-1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant rat Neuropilin‑2
Gln23-Asp857 (Val809-Asp825 del)
Accession # O35276
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Simple Western
5 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 2.5-15 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of Recombinant Human VEGF165 (Catalog # 293-VE) to immobilized Recombinant Rat Neuropilin-2 Fc Chimera (Catalog # 567-N2) coated at 5 µg/mL (100 µL/well). At 60 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse and Rat Neuropilin-2 antibody by Western Blot. View Larger

Detection of Mouse and Rat Neuropilin‑2 by Western Blot. Western blot shows lysates of C6 rat glioma cell line, LL/2 mouse Lewis lung carcinoma cell line, and bEnd.3 mouse endothelioma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse/Rat Neuropilin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF567) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Neuropilin-2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry Neuropilin-2 antibody in Rat Brain by Immunohistochemistry (IHC-Fr). View Larger

Neuropilin‑2 in Rat Brain. Neuropilin-2 was detected in perfusion fixed frozen sections of rat brain using Goat Anti-Mouse/Rat Neuropilin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF567) at 15 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm in neurons. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Simple Western Detection of Mouse and Rat Neuropilin-2 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse and Rat Neuropilin‑2 by Simple WesternTM. Simple Western lane view shows lysates of C6 rat glioma cell line, LL/2 mouse Lewis lung carcinoma cell line, and bEnd.3 mouse endothelioma cell line, loaded at 0.2 mg/mL. A specific band was detected for Neuropilin-2 at approximately 140 kDa (as indicated) using 5 µg/mL of Goat Anti-Mouse/Rat Neuropilin-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF567) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuropilin-2

Neuropilin-1 (Npn-1, previously known as Neuropilin) and Npn-2 (previously known as Npn-1-related molecule) are type I transmembrane proteins that bind members of the class III secreted semaphorin subfamily which are implicated in repulsive axon guidance. The extracellular domain of these proteins is composed of two N-terminal CUB (complement-binding) domains (domains a1 and a2), two domains with homology to coagulation factors V and VIII (domains b1 and b2) and a MAM (meprin) domain (domain c). In the absence of ligands, neuropilins can form homo- and hetero-oligomers via homophilic interactions of their MAM domains. At the amino acid sequence level, Npn-1and Npn-2 share 44% identity. Npn-1 and Npn-2 show different binding specificities for different members of the semaphorin family. The expression patterns of Npn-1 and Npn-2 in developing neurons of the central and peripheral nervous systems are largely, though not completely nonoverlapping. Npn‑1 and Npn-2 are also expressed by endothelial and tumor cells and have been shown to be isoform-specific receptors for VEGF165. Npn‑1 was also reported to bind PlGF-2 and the VEGF-like protein from of virus NZ2.

References
  1. Fujisawa, H. and T. Kitsukawa (1998) Curr. Opin. Neurobiol. 8:587.
  2. Neufeld, G. et al. (1999) FASEB J. 13:9.
  3. Poltorak, Z. et al. (2000) J. Biol. Chem. 275:18040.
Entrez Gene IDs
8828 (Human); 18187 (Mouse); 81527 (Rat)
Alternate Names
MGC126574; neuropilin 2; Neuropilin2; Neuropilin-2; neuropilin-2a(22); neuropilin-2b(0); NP2; NPN 2; NPN2; PRO2714; receptor for VEGF165 and semaphorins class3; Vascular endothelial cell growth factor 165 receptor 2; vascular endothelial growth factor-165 receptor 2; VEGF1265R2; VEGF165R2neuropilin-2a(17)

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Product Specific Notices

This product or the use of this product is covered by U.S. Patents owned by The Regents of the University of California. This product is for research use only and is not to be used for commercial purposes. Use of this product to produce products for sale or for diagnostic, therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for such purposes, contact The Regents of the University of California.

U.S. Patent # 6,054,293, 6,623,738, and other U.S. and international patents pending.

Citations for Mouse/Rat Neuropilin-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

39 Citations: Showing 1 - 10
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  1. Automated quantification of vomeronasal glomeruli number, size, and color composition after immunofluorescent staining
    Authors: Shahab Bahreini Jangjoo, Jennifer M Lin, Farhood Etaati, Sydney Fearnley, Jean-François Cloutier, Alexander Khmaladze et al.
    Chemical Senses
  2. Sociosexual behavior requires both activating and repressive roles of Tfap2e/AP-2 epsilon in vomeronasal sensory neurons
    Authors: Jennifer M Lin, Tyler A Mitchell, Megan Rothstein, Alison Pehl, Ed Zandro M Taroc, Raghu R Katreddi et al.
    eLife
  3. Neuropilin2 regulates the guidance of post-crossing spinal commissural axons in a subtype-specific manner
    Authors: Tracy S Tran, Edward Carlin, Ruihe Lin, Edward Martinez, Jane E Johnson, Zaven Kaprielian
    Neural Development
  4. R-propranolol is a small molecule inhibitor of the SOX18 transcription factor in a rare vascular syndrome and hemangioma
    Authors: Jeroen Overman, Frank Fontaine, Jill Wylie-Sears, Mehdi Moustaqil, Lan Huang, Marie Meurer et al.
    eLife
  5. Paraxial Mesoderm Is the Major Source of Lymphatic Endothelium
    Authors: Oliver A. Stone, Didier Y.R. Stainier
    Developmental Cell
  6. HHEX is a transcriptional regulator of the VEGFC/FLT4/PROX1 signaling axis during vascular development
    Authors: S Gauvrit, A Villasenor, B Strilic, P Kitchen, MM Collins, R Marín-Juez, S Guenther, HM Maischein, N Fukuda, MA Canham, JM Brickman, CW Bogue, PS Jayaraman, DYR Stainier
    Nat Commun, 2018-07-13;9(1):2704.
  7. Loss of Primary Cilia Protein IFT20 Dysregulates Lymphatic Vessel Patterning in Development and Inflammation
    Authors: Delayna Paulson, Rebecca Harms, Cody Ward, Mackenzie Latterell, Gregory J. Pazour, Darci M. Fink
    Frontiers in Cell and Developmental Biology
  8. Mitochondrial respiration controls the Prox1-Vegfr3 feedback loop during lymphatic endothelial cell fate specification and maintenance
    Authors: Wanshu Ma, Hyea Jin Gil, Xiaolei Liu, Lauren P. Diebold, Marc A. Morgan, Michael J. Oxendine-Burns et al.
    Science Advances
  9. Heterogeneity in VEGFR3 levels drives lymphatic vessel hyperplasia through cell-autonomous and non-cell-autonomous mechanisms
    Authors: Y Zhang, MH Ulvmar, L Stanczuk, I Martinez-C, M Frye, K Alitalo, T Mäkinen
    Nat Commun, 2018-04-03;9(1):1296.
  10. Smad4-dependent morphogenic signals control the maturation and axonal targeting of basal vomeronasal sensory neurons to the accessory olfactory bulb
    Authors: Ankana S. Naik, Jennifer M. Lin, Ed Zandro M. Taroc, Raghu R. Katreddi, Jesus A. Frias, Alex A. Lemus et al.
    Development
  11. Opposing Effects of Neuropilin-1 and -2 on Sensory Nerve Regeneration in Wounded Corneas: Role of Sema3C in Ameliorating Diabetic Neurotrophic Keratopathy
    Authors: Patrick Shean-Young Lee, Nan Gao, Mamata Dike, Olga Shkilnyy, Rao Me, Yangyang Zhang et al.
    Diabetes
  12. The blood vasculature instructs lymphatic patterning in a SOX7‐dependent manner
    Authors: Ivy K N Chiang, Matthew S Graus, Nils Kirschnick, Tara Davidson, Winnie Luu, Richard Harwood et al.
    The EMBO Journal
  13. Ankyrin B Promotes Developmental Spine Regulation in the Mouse Prefrontal Cortex
    Authors: Murphy, KE;Duncan, BW;Sperringer, JE;Zhang, EY;Haberman, VA;Wyatt, EV;Maness, PF;
    bioRxiv : the preprint server for biology
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  14. S1PR1 regulates the quiescence of lymphatic vessels by inhibiting laminar shear stress-dependent VEGF-C signaling
    Authors: X Geng, K Yanagida, RG Akwii, D Choi, L Chen, Y Ho, B Cha, MR Mahamud, K Berman de, H Ichise, H Chen, J Wythe, CM Mikelis, T Hla, RS Srinivasan
    JCI Insight, 2020-07-23;0(0):.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Close Homolog of L1 Regulates Dendritic Spine Density in the Mouse Cerebral Cortex through Semaphorin 3B
    Authors: V Mohan, SD Wade, CS Sullivan, MR Kasten, C Sweetman, R Stewart, Y Truong, M Schachner, PB Manis, PF Maness
    J. Neurosci., 2019-06-10;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Immunoprecipitation
  16. Matrix stiffness controls lymphatic vessel formation through regulation of a GATA2-dependent transcriptional program
    Authors: M Frye, A Taddei, C Dierkes, I Martinez-C, M Fielden, H Ortsäter, J Kazenwadel, DP Calado, P Ostergaard, M Salminen, L He, NL Harvey, F Kiefer, T Mäkinen
    Nat Commun, 2018-04-17;9(1):1511.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Apelin modulates pathological remodeling of lymphatic endothelium after myocardial infarction
    Authors: F Tatin, E Renaud-Gab, AC Godet, F Hantelys, F Pujol, F Morfoisse, D Calise, F Viars, P Valet, B Masri, AC Prats, B Garmy-Susi
    JCI Insight, 2017-06-15;2(12):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Sympathetic nerve repulsion inhibited by designer molecules in vitro and role in experimental arthritis
    Authors: Rainer H Straub
    J. Med. Microbiol., 2016-11-14;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  19. Mechanotransduction activates canonical Wnt/?-catenin signaling to promote lymphatic vascular patterning and the development of lymphatic and lymphovenous valves
    Authors: Boksik Cha
    Genes Dev, 2016-06-16;30(12):1454-69.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  20. Pdgfrb-Cre targets lymphatic endothelial cells of both venous and non-venous origins
    Authors: MH Ulvmar, I Martinez-C, L Stanczuk, T Mäkinen
    Genesis, 2016-04-21;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. Neural cell adhesion molecule NrCAM regulates Semaphorin 3F-induced dendritic spine remodeling.
    Authors: Demyanenko G, Mohan V, Zhang X, Brennaman L, Dharbal K, Tran T, Manis P, Maness P
    J Neurosci, 2014-08-20;34(34):11274-87.
    Species: Mouse
    Sample Types: Cell Lysates, Tissue Homogenates, Whole Tissue
    Applications: IHC, Immunoprecipitation
  22. Preferential lymphatic growth in bronchus-associated lymphoid tissue in sustained lung inflammation.
    Authors: Baluk P, Adams A, Phillips K, Feng J, Hong Y, Brown M, McDonald D
    Am J Pathol, 2014-03-11;184(5):1577-92.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Control of retinoid levels by CYP26B1 is important for lymphatic vascular development in the mouse embryo.
    Authors: Bowles J, Secker G, Nguyen C, Kazenwadel J, Truong V, Frampton E, Curtis C, Skoczylas R, Davidson T, Miura N, Hong Y, Koopman P, Harvey N, Francois M
    Dev Biol, 2013-12-19;386(1):25-33.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  24. Direct transcriptional regulation of neuropilin-2 by COUP-TFII modulates multiple steps in murine lymphatic vessel development.
    Authors: Lin FJ, Chen X, Qin J, Hong YK, Tsai MJ, Tsai SY
    J. Clin. Invest., 2010-04-01;120(5):1694-707.
    Species: Mouse
    Sample Types: Cell Culture Supernates, Whole Tissue
    Applications: IHC-P, Western Blot
  25. Bone marrow cells recruited through the neuropilin-1 receptor promote arterial formation at the sites of adult neoangiogenesis in mice.
    Authors: Zacchigna S, Pattarini L, Zentilin L, Moimas S, Carrer A, Sinigaglia M, Arsic N, Tafuro S, Sinagra G, Giacca M
    J. Clin. Invest., 2008-06-01;118(6):2062-75.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  26. Semaphorin 3F confines ventral tangential migration of lateral olfactory tract neurons onto the telencephalon surface.
    Authors: Ito K, Kawasaki T, Takashima S, Matsuda I, Aiba A, Hirata T
    J. Neurosci., 2008-04-23;28(17):4414-22.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  27. BIG-2 mediates olfactory axon convergence to target glomeruli.
    Authors: Kaneko-Goto T, Yoshihara S, Miyazaki H, Yoshihara Y
    Neuron, 2008-03-27;57(6):834-46.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  28. Reconstitution of the embryonic kidney identifies a donor cell contribution to the renal vasculature upon transplantation
    Authors: Y Murakami, H Naganuma, S Tanigawa, T Fujimori, M Eto, R Nishinakam
    Sci Rep, 2019-02-04;9(1):1172.
  29. Tamoxifen-independent recombination of reporter genes limits lineage tracing and mosaic analysis using CreERT2 lines
    Authors: A. Álvarez-Aznar, I. Martínez-Corral, N. Daubel, C. Betsholtz, T. Mäkinen, K. Gaengel
    Transgenic Research
  30. Transient loss of venous integrity during developmental vascular remodeling leads to red blood cell extravasation and clearance by lymphatic vessels
    Authors: Yang Zhang, Nina Daubel, Simon Stritt, Taija Mäkinen
    Development
  31. Floor plate-derived neuropilin-2 functions as a secreted semaphorin sink to facilitate commissural axon midline crossing
    Authors: Berenice Hernandez-Enriquez, Zhuhao Wu, Edward Martinez, Olav Olsen, Zaven Kaprielian, Patricia F. Maness et al.
    Genes & Development
  32. Role of Notch1 in the arterial specification and angiogenic potential of mouse embryonic stem cell-derived endothelial cells
    Authors: Jae Kyung Park, Tae Wook Lee, Eun Kyoung Do, Hye Ji Moon, Jae Ho Kim
    Stem Cell Research & Therapy
  33. Refuting the hypothesis that semaphorin-3f/neuropilin-2 exclude blood vessels from the cap mesenchyme in the developing kidney
    Authors: David A. D. Munro, Peter Hohenstein, Thomas M. Coate, Jamie A. Davies
    Developmental Dynamics
  34. SMAD4 prevents flow induced arterial-venous malformations by inhibiting Casein Kinase 2
    Authors: Roxana Ola, Sandrine H. Künzel, Feng Zhang, Gael Genet, Raja Chakraborty, Laurence Pibouin-Fragner et al.
    Circulation
  35. Chemico-genetic discovery of astrocytic control of inhibition in vivo
    Authors: T Takano, JT Wallace, KT Baldwin, AM Purkey, A Uezu, JL Courtland, EJ Soderblom, T Shimogori, PF Maness, C Eroglu, SH Soderling
    Nature, 2020-11-11;0(0):.
  36. Cdh5-lineage–independent origin of dermal lymphatics shown by temporally restricted lineage tracing
    Authors: Yan Zhang, Henrik Ortsäter, Ines Martinez-Corral, Taija Mäkinen
    Life Science Alliance
  37. Discovery of pan-VEGF inhibitory peptides directed to the extracellular ligand-binding domains of the VEGF receptors
    Authors: Jussara S. Michaloski, Alexandre R. Redondo, Leila S. Magalhães, Caio C. Cambui, Ricardo J. Giordano
    Science Advances
  38. Smooth muscle cell recruitment to lymphatic vessels requires PDGFB and impacts vessel size but not identity
    Authors: Yixin Wang, Yi Jin, Maarja Andaloussi Mäe, Yang Zhang, Henrik Ortsäter, Christer Betsholtz et al.
    Development
  39. NrCAM Deletion Causes Topographic Mistargeting of Thalamocortical Axons to the Visual Cortex and Disrupts Visual Acuity
    Authors: Galina P. Demyanenko, Thorfinn T. Riday, Tracy S. Tran, Jasbir Dalal, Eli P. Darnell, Leann H. Brennaman et al.
    The Journal of Neuroscience

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Reviews for Mouse/Rat Neuropilin-2 Antibody

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Mouse/Rat Neuropilin-2 Antibody
By Anonymous on 12/03/2020
Application: Immunocytochemistry/Immunofluorescence Sample Tested: E12.5 mouse embryo fixed in 4% PFA Species: Mouse

Antibody was stained on E12.5 mouse sections (attached picture) as well as E9.5 mouse sections.
Worked well.
Note the positive stain in the veins and the negative signal in the adjacent arteries.
Dilution used - 1:20

Immunocytochemistry/Immunofluorescence Mouse/Rat Neuropilin-2 Antibody AF567
Mouse/Rat Neuropilin-2 Antibody
By Anonymous on 11/08/2016
Application: IHC Sample Tested: Embryonic tissue Species: Mouse
Immunohistochemistry Mouse/Rat Neuropilin-2 Antibody AF567
Mouse/Rat Neuropilin-2 Antibody
By Anonymous on 09/07/2016
Application: Whole mount immunofluorescence Sample Tested: Whole mount eye tissue Species: Mouse

Whole mouse eye tissue was fixed in 2% PFA, blocked and incubated O/N in AF567 diluted 1:250. Signal was detected using an alexafluor 488-labeled donkey anti goat secondary

Other Mouse/Rat Neuropilin-2 Antibody AF567