Mouse/Rat P-Selectin/CD62P Antibody

Detection of Mouse and Rat P-Selectin/CD62P by Western Blot. Western blot shows lysates of mouse platelets and rat platelets. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse/Rat P-Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF737) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for P-Selectin/CD62P at approximately 140-150 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

P‑Selectin/CD62P in Mouse Liver. P-Selectin/CD62P was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse/Rat P-Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF737) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to endothelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Detection of Rat P-Selectin/CD62P by Simple Western^TM. Simple Western lane view shows lysates of rat platelets, loaded at 0.2 mg/mL. A specific band was detected for P-Selectin/CD62P at approximately 197 kDa (as indicated) using 20 µg/mL of Goat Anti-Mouse P-Selectin/CD62P Antigen Affinity-purified Polyclonal Antibody (Catalog # AF737) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Detection of Mouse P-Selectin/CD62P by Immunocytochemistry/ Immunofluorescence In-vivo targeting of BYL719-loaded nanoparticles prepared with either fucoidan (Fi) or dextran sulfate (Dex).(a) Representative ex vivo fluorescence images of mice organs 24 h after i.v. administration of FiBYL719 or DexBYL719 nanoparticles, and pre-treated with anti-P-selectin antibody (Ab). (b) Nanoparticle biodistribution in organs and tumour, calculated from ex vivo fluorescence images shown in a as total fluorescence efficiency divided by organ weight (n=3). (c) Representative immunofluorescence staining for CD-31 (red), P-selectin (green) and DAPI (blue) in Cal-33 xenografts before and after a single dose of ionizing radiation (4 Gy). Scale bars, 50 μm. (d) Quantification of double-staining positive endothelial cells per tumour shown in c (n=3). (e) In vivo fluorescence imaging of Cal-33 xenograft-bearing mice 24 h after treatment with FiBYL719 or 4 Gy RT followed by FiBYL719. (f) Quantification of total fluorescence efficiency of tumours shown e (n=10). (g) Representative immunofluorescence stains of tumour sections for P-selectin (green), NIR (red) and DAPI (blue) from H22 xenografts 24 h after treatments. Scale bars, 50 μm. In b,d,f error bars indicate mean±s.e.m. *P<0.05, **P<0.01; by Mann–Whitney U-test in b or one-way ANOVA with post hoc Tukey test in d,f. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/ncomms14292), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse P-Selectin/CD62P by Immunocytochemistry/ Immunofluorescence In-vivo targeting of BYL719-loaded nanoparticles prepared with either fucoidan (Fi) or dextran sulfate (Dex).(a) Representative ex vivo fluorescence images of mice organs 24 h after i.v. administration of FiBYL719 or DexBYL719 nanoparticles, and pre-treated with anti-P-selectin antibody (Ab). (b) Nanoparticle biodistribution in organs and tumour, calculated from ex vivo fluorescence images shown in a as total fluorescence efficiency divided by organ weight (n=3). (c) Representative immunofluorescence staining for CD-31 (red), P-selectin (green) and DAPI (blue) in Cal-33 xenografts before and after a single dose of ionizing radiation (4 Gy). Scale bars, 50 μm. (d) Quantification of double-staining positive endothelial cells per tumour shown in c (n=3). (e) In vivo fluorescence imaging of Cal-33 xenograft-bearing mice 24 h after treatment with FiBYL719 or 4 Gy RT followed by FiBYL719. (f) Quantification of total fluorescence efficiency of tumours shown e (n=10). (g) Representative immunofluorescence stains of tumour sections for P-selectin (green), NIR (red) and DAPI (blue) from H22 xenografts 24 h after treatments. Scale bars, 50 μm. In b,d,f error bars indicate mean±s.e.m. *P<0.05, **P<0.01; by Mann–Whitney U-test in b or one-way ANOVA with post hoc Tukey test in d,f. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/ncomms14292), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of Mouse P-Selectin/CD62P by Immunocytochemistry/ Immunofluorescence In-vivo targeting of BYL719-loaded nanoparticles prepared with either fucoidan (Fi) or dextran sulfate (Dex).(a) Representative ex vivo fluorescence images of mice organs 24 h after i.v. administration of FiBYL719 or DexBYL719 nanoparticles, and pre-treated with anti-P-selectin antibody (Ab). (b) Nanoparticle biodistribution in organs and tumour, calculated from ex vivo fluorescence images shown in a as total fluorescence efficiency divided by organ weight (n=3). (c) Representative immunofluorescence staining for CD-31 (red), P-selectin (green) and DAPI (blue) in Cal-33 xenografts before and after a single dose of ionizing radiation (4 Gy). Scale bars, 50 μm. (d) Quantification of double-staining positive endothelial cells per tumour shown in c (n=3). (e) In vivo fluorescence imaging of Cal-33 xenograft-bearing mice 24 h after treatment with FiBYL719 or 4 Gy RT followed by FiBYL719. (f) Quantification of total fluorescence efficiency of tumours shown e (n=10). (g) Representative immunofluorescence stains of tumour sections for P-selectin (green), NIR (red) and DAPI (blue) from H22 xenografts 24 h after treatments. Scale bars, 50 μm. In b,d,f error bars indicate mean±s.e.m. *P<0.05, **P<0.01; by Mann–Whitney U-test in b or one-way ANOVA with post hoc Tukey test in d,f. Image collected and cropped by CiteAb from the following open publication (https://www.nature.com/articles/ncomms14292), licensed under a CC-BY license. Not internally tested by R&D Systems.