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Mouse/Rat PDX-1/IPF1 Antibody

Catalog #: AF2517
8 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2517
AF2517-SP
Detection of Mouse/Rat PDX‑1/IPF1 by Western Blot.
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Citations (8)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse/Rat PDX-1/IPF1 Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse PDX‑1/IPF1 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse PDX‑1/IPF1
Met1-Arg284
Accession # P52946
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
10 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse/Rat PDX‑1/IPF1 antibody by Western Blot. View Larger

Detection of Mouse/Rat PDX‑1/IPF1 by Western Blot. Western blot shows lysates of beta TC-6 mouse beta cell insulinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse/Rat PDX-1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2517) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PDX-1/IPF1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunocytochemistry PDX-1/IPF1 antibody in beta TC-6 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

PDX‑1/IPF1 in beta TC‑6 Mouse Cell Line. PDX-1/IPF1 was detected in immersion fixed beta TC-6 mouse beta cell insulinoma cell line using Goat Anti-Mouse/Rat PDX-1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2517) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to the nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Simple Western Detection of Mouse PDX‑1/IPF1 antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Mouse PDX‑1/IPF1 by Simple WesternTM. Simple Western lane view shows lysates of beta TC-6 mouse beta cell insulinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for PDX-1/IPF1 at approximately 48 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse/Rat PDX-1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2517) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunohistochemistry PDX-1/IPF1 antibody in Mouse Pancreas by Immunohistochemistry (IHC-Fr). View Larger

PDX‑1/IPF1 in Mouse Pancreas. PDX-1/IPF1 was detected in perfusion fixed frozen sections of mouse pancreas using Goat Anti-Mouse/Rat PDX-1/IPF1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2517) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDX-1/IPF1

PDX-1 (Pancreas-duodenum homeobox 1; also IPF-1, IDX-1, STF-1, GSF and IUF-1) is a 42 kDa member of the IPF-1 subfamily, ANTP homeobox family of proteins. It is expressed in developing pancreas, plus pancreatic beta -cells, and duodenal crypt epithelium. In concert with HNF6, PDX-1 drives pancreatic endocrine cell formation by regulating the expression of neurogenin-3. In adult beta -cells, PDX-1 binds to PCIF-1 to regulate insulin production. Mouse PDX-1 is 284 amino acids (aa) in length (Accession # P52946). It contains one transactivation domain (aa 13‑73) a PBX1 P-P-M-W-K dimerization motif (aa 119‑124), and a DNA-binding homeodomain with an embedded NLS (aa 148‑206). Full-length mouse PDX-1 shares 94% and 88% aa identity with rat and human PDX-1, respectively.

Long Name
Pancreas/Duodenum Homeobox-1
Entrez Gene IDs
3651 (Human); 18609 (Mouse)
Alternate Names
Glucose-sensitive factor; IDX1; IDX-1GSF; Insulin promoter factor 1; insulin promoter factor 1, homeodomain transcription factor; Insulin upstream factor 1; IPF1; IPF1pancreas/duodenum homeobox protein 1; Islet/duodenum homeobox-1; MODY4IUF1; pancreatic and duodenal homeobox 1; pancreatic-duodenal homeobox factor 1; PDX-1; PDX-1IPF-1; somatostatin transcription factor 1; Somatostatin-transactivating factor 1; STF-1IUF-1

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Citations for Mouse/Rat PDX-1/IPF1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Identification of dynamic undifferentiated cell states within the male germline
    Authors: HM La, JA Mäkelä, AL Chan, FJ Rossello, CM Nefzger, JMD Legrand, M De Seram, JM Polo, RM Hobbs
    Nat Commun, 2018-07-19;9(1):2819.
  2. Activated dormant stem cells recover spermatogenesis in chemoradiotherapy-induced infertility
    Authors: Yang, SH;Zeng, YZ;Jia, XZ;Gu, YW;Wood, C;Yang, RS;Yang, JS;Yang, WJ;
    Cell reports
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  3. Structural and immunoendocrine remodeling in gut, pancreas and thymus in weaning rats fed powdered milk diets rich in Maillard reactants
    Authors: J Dereke, E Ekblad, B Weström, C Erlanson-A, M Landin-Ols, I Sjöholm, M Hillman
    Scientific Reports, 2022-03-08;12(1):4039.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  4. Costus pictus D. Don leaf extract stimulates GLP-1 secretion from GLUTag L-cells and has cytoprotective effects in BRIN-BD11 beta -cells
    Authors: Chinmai Patibandla, Zahidul Islam Khan, Louise MacGregor, Mark James Campbell, Steven Patterson
    Journal of Ethnopharmacology
  5. Characterization of induced tissue-specific stem cells from pancreas by a synthetic self-replicative RNA
    Authors: C Miyagi-Shi, Y Nakashima, N Kobayashi, I Saitoh, M Watanabe, H Noguchi
    Sci Rep, 2018-08-17;8(1):12341.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  6. Expression of pancreatic and duodenal homeobox1 (PDX1) protein in the interior and exterior regions of the intestine, revealed by development and analysis of Pdx1 knockout mice
    Authors: Haruo Hashimoto, Tsutomu Kamisako, Takahiro Kagawa, Seiki Haraguchi, Mika Yagoto, Ri-ichi Takahashi et al.
    Laboratory Animal Research
  7. Per-Arnt-Sim kinase regulates pancreatic duodenal homeobox-1 protein stability via phosphorylation of glycogen synthase kinase 3beta in pancreatic beta-cells.
    Authors: Semache M, Zarrouki B, Fontes G, Fogarty S, Kikani C, Chawki M, Rutter J, Poitout V
    J Biol Chem, 2013-07-12;288(34):24825-33.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Self-renewal of embryonic-stem-cell-derived progenitors by organ-matched mesenchyme.
    Authors: Sneddon, Julie B, Borowiak, Malgorza, Melton, Douglas
    Nature, 2012-10-07;491(7426):765-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC

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