Mouse/Rat RAGE Antibody Summary
Gly23-Ala342
Accession # NP_031451
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
Detection of Mouse RAGE by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse/Rat RAGE Monoclonal Antibody (Catalog # 1179) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for RAGE and mature RAGE at approximately 50 and 38 kDa, respectively (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
RAGE in Mouse Lung. RAGE was detected in perfusion fixed frozen sections of adult mouse lung using Rat Anti-Mouse/Rat RAGE Monoclonal Antibody (Catalog # MAB1179) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Northern-Lights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RAGE/AGER
Advanced glycation endproducts (AGE) are adducts formed by the non-enzymatic glycation or oxidation of macromolecules (1). AGE forms during aging and its formation is accelerated under pathophysiologic states such as diabetes, Alzheimer’s disease, renal failure and immune/inflammatory disorders. Receptor for Advanced Glycation Endoproducts (RAGE), named for its ability to bind AGE, is a multiligand receptor belonging the immunoglobulin (Ig) superfamily. Besides AGE, RAGE binds amyloid beta -peptide, S100/calgranulin family proteins, high mobility group B1 (HMGB1, also know as amphoterin) and leukocyte integrins (1, 2). The mouse RAGE gene encodes a 403 amino acid (aa) residue type I transmembrane glycoprotein with a 22 aa signal peptide, a 319 aa extracellular domain containing a Ig-like V‑type domain and two Ig-like Ce-type domains, a 21 aa transmembrane domain and a 41 aa cytoplasmic domain (3). The V‑type domain and the cytoplasmic domain are important for ligand binding and for intracellular signaling, respectively. Two alternative splice variants, lacking the V‑type domain or the cytoplasmic tail, are known (1, 4). RAGE is highly expressed in the embryonic central nervous system (5). In adult tissues, RAGE is expressed at low levels in multiple tissues including endothelial and smooth muscle cells, mononuclear phagocytes, pericytes, microglia, neurons, cardiac myocytes and hepatocytes (6). The expression of RAGE is upregulated upon ligand interaction. Depending on the cellular context and interacting ligand, RAGE activation can trigger differential signaling pathways that affect divergent pathways of gene expression (1, 7). RAGE activation modulates varied essential cellular responses (including inflammation, immunity, proliferation, cellular adhesion and migration) that contribute to cellular dysfunction associated with chronic diseases such as diabetes, cancer, amyloidoses, and immune or inflammatory disorders (1).
- Schmidt, A. et al. (2001) J. Clin. Invest. 108:949.
- Chavakis, T. et al. (2003) J. Exp. Med. 198:507.
- Renard, C. et al. (1997) Mol. Pharmacol. 52:54.
- Yonekura, H. et al. (2003) Biochem. J. 370:1097.
- Hori, O. et al. (1995) J. Biol. Chem. 270:25752.
- Brett, J. et al. (1993) Am. J. Pathol. 143:1699.
- Valencia, J.V. et al. (2004) Diabetes 53:743.
Product Datasheets
Citations for Mouse/Rat RAGE Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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EHMT2 methyltransferase governs cell identity in the lung and is required for KRAS G12D tumor development and propagation
Authors: Ariel Pribluda, Anneleen Daemen, Anthony Nelson Lima, Xi Wang, Marc Hafner, Chungkee Poon et al.
eLife
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Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis.
Authors: Kobayashi, Y, Tata, A Et al.
Nat Cell Biol
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Induction of RAGE Shedding by Activation of G Protein-Coupled Receptors
Authors: Verena V. Metz, Elzbieta Kojro, Dorothea Rat, Rolf Postina
PLoS ONE
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KRAS(G12D) drives lepidic adenocarcinoma through stem-cell reprogramming
Authors: Juul NH, Yoon JK, Martinez MC et al.
Nature
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Defined conditions for long-term expansion of murine and human alveolar epithelial stem cells in three-dimensional cultures
Authors: Satoshi Konishi, Aleksandra Tata, Purushothama Rao Tata
STAR Protocols
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Temporal and spatial staging of lung alveolar regeneration is determined by the grainyhead transcription factor Tfcp2l1
Authors: Cardenas-Diaz FL, Liberti DC, Leach JP et al.
Cell reports
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The importance of nuclear RAGE-Mcm2 axis in diabetes or cancer-associated replication stress
Authors: Han Z, Andr� M, Madhavan BK et al.
Nucleic acids research
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Airway secretory cell fate conversion via YAP-mTORC1-dependent essential amino acid metabolism
Authors: Jeon HY, Choi J, Kraaier L et al.
The EMBO journal
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Isolation and culture of mouse alveolar type II cells to study type II to type I cell differentiation
Authors: Qian Chen, Yuru Liu
STAR Protocols
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Epithelial Vegfa Specifies a Distinct Endothelial Population in the Mouse Lung
Authors: Lisandra Vila Ellis, Margo P. Cain, Vera Hutchison, Per Flodby, Edward D. Crandall, Zea Borok et al.
Developmental Cell
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Fibrillin-2 is a key mediator of smooth muscle extracellular matrix homeostasis during mouse tracheal tubulogenesis
Authors: Wenguang Yin, Hyun-Taek Kim, ShengPeng Wang, Felix Gunawan, Rui Li, Carmen Buettner et al.
European Respiratory Journal
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Decreased Expression of Pulmonary Homeobox NKX2.1 and Surfactant Protein C in Developing Lungs That Over-Express Receptors for Advanced Glycation End-Products (RAGE)
Authors: Clarke, DM;Curtis, KL;Wendt, RA;Stapley, BM;Clark, ET;Beckett, N;Campbell, KM;Arroyo, JA;Reynolds, PR;
Journal of developmental biology
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
p53 governs an AT1 differentiation programme in lung cancer suppression
Authors: Kaiser, AM;Gatto, A;Hanson, KJ;Zhao, RL;Raj, N;Ozawa, MG;Seoane, JA;Bieging-Rolett, KT;Wang, M;Li, I;Trope, WL;Liou, DZ;Shrager, JB;Plevritis, SK;Newman, AM;Van Rechem, C;Attardi, LD;
Nature
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Hippo signaling impairs alveolar epithelial regeneration in pulmonary fibrosis
Authors: Warren, R;Lyu, H;Klinkhammer, K;De Langhe, SP;
eLife
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Prenatal triclosan exposure impairs mammalian lung branching morphogenesis through activating Bmp4 signaling
Authors: Q Li, Y Qiao, F Wang, J Zhao, L Wu, H Ge, S Xu
Ecotoxicology and environmental safety, 2023-04-12;256(0):114896.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
The importance of nuclear RAGE-Mcm2 axis in diabetes or cancer-associated replication stress
Authors: Han Z, Andr� M, Madhavan BK et al.
Nucleic acids research
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Alveolar cell fate selection and lifelong maintenance of AT2 cells by FGF signaling
Authors: DG Brownfield, AD de Arce, E Ghelfi, A Gillich, TJ Desai, MA Krasnow
Nature Communications, 2022-11-21;13(1):7137.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Comparison of the pathogenesis of SARS-CoV-2 infection in K18-hACE2 mice and Syrian golden hamster models
Authors: H Jeong, YW Lee, IH Park, H Noh, SH Kim, J Kim, D Jeon, HJ Jang, J Oh, D On, C Uhm, K Cho, H Oh, S Yoon, JS Seo, JJ Kim, SH Seok, YJ Lee, SM Hong, SH An, SY Kim, YB Kim, JY Hwang, HJ Lee, HB Kim, DG Jeong, D Song, M Song, MS Park, KS Choi, JW Park, JY Seo, JW Yun, JS Shin, HY Lee, KT Nam, JK Seong
Disease Models & Mechanisms, 2022-11-11;0(0):.
Species: Hamster, Mouse
Sample Types: Whole Tissue
Applications: IHC -
Essential role of IL-17 in acute exacerbation of pulmonary fibrosis induced by non-typeable Haemophilus influenzae
Authors: S Chen, X Zhang, C Yang, S Wang, H Shen
Theranostics, 2022-07-04;12(11):5125-5137.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
A conserved YAP/Notch/REST network controls the neuroendocrine cell fate in the lungs
Authors: YT Shue, AP Drainas, NY Li, SM Pearsall, D Morgan, N Sinnott-Ar, SQ Hipkins, GL Coles, JS Lim, AE Oro, KL Simpson, C Dive, J Sage
Nature Communications, 2022-05-16;13(1):2690.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Heparan sulfate-dependent RAGE oligomerization is indispensable for pathophysiological functions of RAGE
Authors: M Li, CY Ong, CJ Langouët-A, L Tan, A Verma, Y Yang, X Zhang, DK Shah, EP Schmidt, D Xu
Elife, 2022-02-09;11(0):.
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot -
DCN released from ferroptotic cells ignites AGER-dependent immune responses
Authors: J Liu, S Zhu, L Zeng, J Li, DJ Klionsky, G Kroemer, J Jiang, D Tang, R Kang
Autophagy, 2021-12-29;0(0):1-14.
Species: Mouse
Sample Types: Cell Lysates, In Vivo
Applications: Immunoprecipitation, Neutralization, Western Blot -
Differentiation of mouse fetal lung alveolar progenitors in serum-free organotypic cultures
Authors: K Gkatzis, P Panza, S Peruzzo, DY Stainier
Elife, 2021-09-29;10(0):.
Species: Mouse
Sample Types: Organoids
Applications: IHC -
Overexpression of the receptor for advanced glycation end-products in the auditory cortex of rats with noise-induced hearing loss
Authors: CH Lee, KW Kim, DH Lee, SM Lee, SY Kim
Bmc Neuroscience, 2021-05-21;22(1):38.
Species: Rat
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot -
Alveolar epithelial cell fate is maintained in a spatially restricted manner to promote lung regeneration after acute injury
Authors: DC Liberti, MM Kremp, WA Liberti, IJ Penkala, S Li, S Zhou, EE Morrisey
Cell Reports, 2021-05-11;35(6):109092.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Dose-Dependent Effects of Resveratrol on Cisplatin-Induced Hearing Loss
Authors: CH Lee, KW Kim, SM Lee, SY Kim
International Journal of Molecular Sciences, 2020-12-24;22(1):.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Regeneration of the pulmonary vascular endothelium after viral pneumonia requires COUP-TF2
Authors: Gan Zhao, Aaron I. Weiner, Katherine M. Neupauer, Maria Fernanda de Mello Costa, Gargi Palashikar, Stephanie Adams-Tzivelekidis et al.
Science Advances
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Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction
Authors: H Katsura, V Sontake, A Tata, Y Kobayashi, CE Edwards, BE Heaton, A Konkimalla, T Asakura, Y Mikami, EJ Fritch, PJ Lee, NS Heaton, RC Boucher, SH Randell, RS Baric, PR Tata
Cell Stem Cell, 2020-10-21;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Gli1+ mesenchymal stromal cells form a pathological niche to promote airway progenitor metaplasia in the fibrotic lung
Authors: M Cassandras, C Wang, J Kathiriya, T Tsukui, P Matatia, M Matthay, P Wolters, A Molofsky, D Sheppard, H Chapman, T Peng
Nat Cell Biol, 2020-10-12;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Angiocrine Sphingosine-1-Phosphate Activation of S1PR2-YAP Signaling Axis in Alveolar Type II Cells Is Essential for Lung Repair
Authors: Q Chen, J Rehman, M Chan, P Fu, SM Dudek, V Natarajan, AB Malik, Y Liu
Cell Rep, 2020-06-30;31(13):107828.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
TAZ is required for lung alveolar epithelial cell differentiation after injury
Authors: T Sun, Z Huang, H Zhang, C Posner, G Jia, TR Ramalingam, M Xu, HD Brightbill, JG Egen, A Dey, JR Arron
JCI Insight, 2019-06-18;5(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
IL-1 and TNF? Contribute to the Inflammatory Niche to Enhance Alveolar Regeneration
Authors: H Katsura, Y Kobayashi, PR Tata, BLM Hogan
Stem Cell Reports, 2019-03-28;0(0):.
Species: Mouse
Sample Types: Organoids
Applications: IHC-P -
Pulmonary pericytes regulate lung morphogenesis
Authors: K Kato, R Diéguez-Hu, DY Park, SP Hong, S Kato-Azuma, S Adams, M Stehling, B Trappmann, JL Wrana, GY Koh, RH Adams
Nat Commun, 2018-06-22;9(1):2448.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
RAGE-specific single chain Fv for PET imaging of pancreatic cancer
Authors: HY Kim, X Wang, R Kang, D Tang, BA Boone, HJ Zeh, MT Lotze, WB Edwards
PLoS ONE, 2018-03-12;13(3):e0192821.
Species: Mouse
Sample Types: Whole Cells, Whole Tissue
Applications: Flow Cytometry, ICC, IHC -
Therapeutic Effectiveness of Anti-RAGE Antibody Administration in a Rat Model of Crush Injury
Authors: H Matsumoto, N Matsumoto, J Shimazaki, J Nakagawa, Y Imamura, K Yamakawa, T Yamada, M Ikeda, H Hiraike, H Ogura, T Shimazu
Sci Rep, 2017-09-25;7(1):12255.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Recruited Monocytes and Type 2 Immunity Promote Lung Regeneration following Pneumonectomy
Authors: AJ Lechner, IH Driver, J Lee, CM Conroy, A Nagle, RM Locksley, JR Rock
Cell Stem Cell, 2017-05-11;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
The development and plasticity of alveolar type 1 cells
Authors: J Yang, BJ Hernandez, D Martinez A, O Narvaez de, L Vila-Ellis, H Akiyama, SE Evans, EJ Ostrin, J Chen
Development, 2015-11-19;143(1):54-65.
Species: Mouse
Sample Types: Whole Cells
Applications: ICC -
Two nested developmental waves demarcate a compartment boundary in the mouse lung.
Authors: Alanis, Denise M, Chang, Daniel R, Akiyama, Haruhiko, Krasnow, Mark A, Chen, Jichao
Nat Commun, 2014-05-29;5(0):3923.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Alveolar progenitor and stem cells in lung development, renewal and cancer.
Authors: Desai, Tushar J, Brownfield, Douglas, Krasnow, Mark A
Nature, 2014-02-05;507(7491):190-4.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-Fr -
Receptor for advanced glycation end products (RAGE) functions as receptor for specific sulfated glycosaminoglycans, and anti-RAGE antibody or sulfated glycosaminoglycans delivered in vivo inhibit pulmonary metastasis of tumor cells.
Authors: Mizumoto S, Takahashi J, Sugahara K
J. Biol. Chem., 2012-04-09;287(23):18985-94.
Species: Mouse
Sample Types: In Vivo, Whole Cells
Applications: Neutralization -
Aldose reductase and AGE-RAGE pathways: central roles in the pathogenesis of vascular dysfunction in aging rats.
Authors: Hallam KM, Li Q, Ananthakrishnan R, Kalea A, Zou YS, Vedantham S, Schmidt AM, Yan SF, Ramasamy R
Aging Cell, 2010-08-15;9(5):776-84.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Role of soluble receptor for advanced glycation end products on endotoxin-induced lung injury.
Authors: Zhang H, Tasaka S, Shiraishi Y, Fukunaga K, Yamada W, Seki H, Ogawa Y, Miyamoto K, Nakano Y, Hasegawa N, Miyasho T, Maruyama I, Ishizaka A
Am. J. Respir. Crit. Care Med., 2008-06-05;178(4):356-62.
Species: Mouse
Sample Types: BALF
Applications: Western Blot -
Alpha-4/beta-1 and alpha-L/beta-2 integrins mediate cytokine induced lung leukocyte-epithelial adhesion and injury.
Authors: Parmley LA, Elkins ND, Fini MA, Liu YE, Repine JE, Wright RM
Br. J. Pharmacol., 2007-09-10;152(6):915-29.
Species: Rat
Sample Types: BALF
Applications: Western Blot -
Low protein-induced intrauterine growth restriction as a risk factor for schizophrenia phenotype in a rat model: assessing the role of oxidative stress and neuroinflammation interaction
Authors: Larissa Allgäuer, Jan-Harry Cabungcal, Catherine Yzydorczyk, Kim Quang Do, Daniella Dwir
Translational Psychiatry
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delta Np63 drives dysplastic alveolar remodeling and restricts epithelial plasticity upon severe lung injury
Authors: Aaron I. Weiner, Gan Zhao, Hanna M. Zayas, Nicolas P. Holcomb, Stephanie Adams-Tzivelekidis, Joanna Wong et al.
Cell Reports
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Ager-CreERT2: A New Genetic Tool for Studying Lung Alveolar Development, Homeostasis, and Repair
Authors: Mei-I Chung, Brigid L. M. Hogan
American Journal of Respiratory Cell and Molecular Biology
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Nephrin loss is reduced by grape seed proanthocyanidins in the experimental diabetic nephropathy rat model
Authors: Xianhua Li, Zhaoli Gao, Haiqing Gao, Baoying Li, Tao Peng, Bei Jiang et al.
Molecular Medicine Reports
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Regeneration of the pulmonary vascular endothelium after viral pneumonia requires COUP-TF2
Authors: Gan Zhao, Aaron I. Weiner, Katherine M. Neupauer, Maria Fernanda de Mello Costa, Gargi Palashikar, Stephanie Adams-Tzivelekidis et al.
Science Advances
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Mouse models of lung-specific SARS-CoV-2 infection with moderate pathological traits
Authors: Kim SH, Kim J, Jang JY et al.
Frontiers in Immunology
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MMP9/RAGE pathway overactivation mediates redox dysregulation and neuroinflammation, leading to inhibitory/excitatory imbalance: a reverse translation study in schizophrenia patients
Authors: Daniella Dwir, Basilio Giangreco, Lijing Xin, Liliane Tenenbaum, Jan-Harry Cabungcal, Pascal Steullet et al.
Molecular Psychiatry
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Lung epithelial branching program antagonizes alveolar differentiation.
Authors: Chang DR et al.
Proc Natl Acad Sci U S A
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Multi-apical polarity of alveolar stem cells and their dynamics during lung development and regeneration
Authors: Arvind Konkimalla, Satoshi Konishi, Yoshihiko Kobayashi, Preetish Kadur Lakshminarasimha Murthy, Lauren Macadlo, Ananya Mukherjee et al.
iScience
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Persistent Pathology in Influenza-Infected Mouse Lungs
Authors: Cindy M. Kanegai, Ying Xi, Matthew L. Donne, Jeffrey E. Gotts, Ian H. Driver, Gorica Amidzic et al.
American Journal of Respiratory Cell and Molecular Biology
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Distinct Airway Epithelial Stem Cells Hide among Club Cells but Mobilize to Promote Alveolar Regeneration
Authors: JJ Kathiriya, AN Brumwell, JR Jackson, X Tang, HA Chapman
Cell Stem Cell, 2020-01-23;0(0):.
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Automated cell-type classification in intact tissues by single-cell molecular profiling
Authors: Monica Nagendran, Daniel P Riordan, Pehr B Harbury, Tushar J Desai
eLife
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