Mouse/Rat RAGE Antibody

Catalog # Availability Size / Price Qty
MAB1179-SP
MAB1179-100
MAB1179-500
Detection of Mouse RAGE by Western Blot.
2 Images
Product Details
Citations (55)
FAQs
Supplemental Products
Reviews (1)

Mouse/Rat RAGE Antibody Summary

Species Reactivity
Mouse, Rat
Specificity
Detects mouse and rat RAGE in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human RAGE is observed.
Source
Monoclonal Rat IgG2A Clone # 175410
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse RAGE
Gly23-Ala342
Accession # NP_031451
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse RAGE antibody by Western Blot. View Larger

Detection of Mouse RAGE by Western Blot. Western blot shows lysates of mouse lung tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse/Rat RAGE Monoclonal Antibody (Catalog # 1179) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for RAGE and mature RAGE at approximately 50 and 38 kDa, respectively (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry RAGE antibody in Mouse Lung by Immunohistochemistry (IHC-Fr). View Larger

RAGE in Mouse Lung. RAGE was detected in perfusion fixed frozen sections of adult mouse lung using Rat Anti-Mouse/Rat RAGE Monoclonal Antibody (Catalog # MAB1179) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Northern-Lights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RAGE/AGER

Advanced glycation endproducts (AGE) are adducts formed by the non-enzymatic glycation or oxidation of macromolecules (1). AGE forms during aging and its formation is accelerated under pathophysiologic states such as diabetes, Alzheimer’s disease, renal failure and immune/inflammatory disorders. Receptor for Advanced Glycation Endoproducts (RAGE), named for its ability to bind AGE, is a multiligand receptor belonging the immunoglobulin (Ig) superfamily. Besides AGE, RAGE binds amyloid beta -peptide, S100/calgranulin family proteins, high mobility group B1 (HMGB1, also know as amphoterin) and leukocyte integrins (1, 2). The mouse RAGE gene encodes a 403 amino acid (aa) residue type I transmembrane glycoprotein with a 22 aa signal peptide, a 319 aa extracellular domain containing a Ig-like V‑type domain and two Ig-like Ce-type domains, a 21 aa transmembrane domain and a 41 aa cytoplasmic domain (3). The V‑type domain and the cytoplasmic domain are important for ligand binding and for intracellular signaling, respectively. Two alternative splice variants, lacking the V‑type domain or the cytoplasmic tail, are known (1, 4). RAGE is highly expressed in the embryonic central nervous system (5). In adult tissues, RAGE is expressed at low levels in multiple tissues including endothelial and smooth muscle cells, mononuclear phagocytes, pericytes, microglia, neurons, cardiac myocytes and hepatocytes (6). The expression of RAGE is upregulated upon ligand interaction. Depending on the cellular context and interacting ligand, RAGE activation can trigger differential signaling pathways that affect divergent pathways of gene expression (1, 7). RAGE activation modulates varied essential cellular responses (including inflammation, immunity, proliferation, cellular adhesion and migration) that contribute to cellular dysfunction associated with chronic diseases such as diabetes, cancer, amyloidoses, and immune or inflammatory disorders (1).

References
  1. Schmidt, A. et al. (2001) J. Clin. Invest. 108:949.
  2. Chavakis, T. et al. (2003) J. Exp. Med. 198:507.
  3. Renard, C. et al. (1997) Mol. Pharmacol. 52:54.
  4. Yonekura, H. et al. (2003) Biochem. J. 370:1097.
  5. Hori, O. et al. (1995) J. Biol. Chem. 270:25752.
  6. Brett, J. et al. (1993) Am. J. Pathol. 143:1699.
  7. Valencia, J.V. et al. (2004) Diabetes 53:743.
Long Name
Receptor for Advanced Glycation End Products
Entrez Gene IDs
177 (Human); 11596 (Mouse); 81722 (Rat); 403168 (Canine)
Alternate Names
advanced glycosylation end product-specific receptor; AGER; RAGE isoform delta; RAGE isoform sRAGE-delta; RAGE; Receptor for advanced glycosylation end products; receptor for advanced glycosylation end-products; SCARJ1

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Citations for Mouse/Rat RAGE Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

55 Citations: Showing 1 - 10
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  1. EHMT2 methyltransferase governs cell identity in the lung and is required for KRAS G12D tumor development and propagation
    Authors: Ariel Pribluda, Anneleen Daemen, Anthony Nelson Lima, Xi Wang, Marc Hafner, Chungkee Poon et al.
    eLife
  2. Persistence of a regeneration-associated, transitional alveolar epithelial cell state in pulmonary fibrosis.
    Authors: Kobayashi, Y, Tata, A Et al.
    Nat Cell Biol
  3. Induction of RAGE Shedding by Activation of G Protein-Coupled Receptors
    Authors: Verena V. Metz, Elzbieta Kojro, Dorothea Rat, Rolf Postina
    PLoS ONE
  4. KRAS(G12D) drives lepidic adenocarcinoma through stem-cell reprogramming
    Authors: Juul NH, Yoon JK, Martinez MC et al.
    Nature
  5. Defined conditions for long-term expansion of murine and human alveolar epithelial stem cells in three-dimensional cultures
    Authors: Satoshi Konishi, Aleksandra Tata, Purushothama Rao Tata
    STAR Protocols
  6. Temporal and spatial staging of lung alveolar regeneration is determined by the grainyhead transcription factor Tfcp2l1
    Authors: Cardenas-Diaz FL, Liberti DC, Leach JP et al.
    Cell reports
  7. The importance of nuclear RAGE-Mcm2 axis in diabetes or cancer-associated replication stress
    Authors: Han Z, Andr� M, Madhavan BK et al.
    Nucleic acids research
  8. Airway secretory cell fate conversion via YAP-mTORC1-dependent essential amino acid metabolism
    Authors: Jeon HY, Choi J, Kraaier L et al.
    The EMBO journal
  9. Isolation and culture of mouse alveolar type II cells to study type II to type I cell differentiation
    Authors: Qian Chen, Yuru Liu
    STAR Protocols
  10. Epithelial Vegfa Specifies a Distinct Endothelial Population in the Mouse Lung
    Authors: Lisandra Vila Ellis, Margo P. Cain, Vera Hutchison, Per Flodby, Edward D. Crandall, Zea Borok et al.
    Developmental Cell
  11. Fibrillin-2 is a key mediator of smooth muscle extracellular matrix homeostasis during mouse tracheal tubulogenesis
    Authors: Wenguang Yin, Hyun-Taek Kim, ShengPeng Wang, Felix Gunawan, Rui Li, Carmen Buettner et al.
    European Respiratory Journal
  12. Decreased Expression of Pulmonary Homeobox NKX2.1 and Surfactant Protein C in Developing Lungs That Over-Express Receptors for Advanced Glycation End-Products (RAGE)
    Authors: Clarke, DM;Curtis, KL;Wendt, RA;Stapley, BM;Clark, ET;Beckett, N;Campbell, KM;Arroyo, JA;Reynolds, PR;
    Journal of developmental biology
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. p53 governs an AT1 differentiation programme in lung cancer suppression
    Authors: Kaiser, AM;Gatto, A;Hanson, KJ;Zhao, RL;Raj, N;Ozawa, MG;Seoane, JA;Bieging-Rolett, KT;Wang, M;Li, I;Trope, WL;Liou, DZ;Shrager, JB;Plevritis, SK;Newman, AM;Van Rechem, C;Attardi, LD;
    Nature
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Hippo signaling impairs alveolar epithelial regeneration in pulmonary fibrosis
    Authors: Warren, R;Lyu, H;Klinkhammer, K;De Langhe, SP;
    eLife
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  15. Prenatal triclosan exposure impairs mammalian lung branching morphogenesis through activating Bmp4 signaling
    Authors: Q Li, Y Qiao, F Wang, J Zhao, L Wu, H Ge, S Xu
    Ecotoxicology and environmental safety, 2023-04-12;256(0):114896.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. The importance of nuclear RAGE-Mcm2 axis in diabetes or cancer-associated replication stress
    Authors: Han Z, Andr� M, Madhavan BK et al.
    Nucleic acids research
  17. Alveolar cell fate selection and lifelong maintenance of AT2 cells by FGF signaling
    Authors: DG Brownfield, AD de Arce, E Ghelfi, A Gillich, TJ Desai, MA Krasnow
    Nature Communications, 2022-11-21;13(1):7137.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Comparison of the pathogenesis of SARS-CoV-2 infection in K18-hACE2 mice and Syrian golden hamster models
    Authors: H Jeong, YW Lee, IH Park, H Noh, SH Kim, J Kim, D Jeon, HJ Jang, J Oh, D On, C Uhm, K Cho, H Oh, S Yoon, JS Seo, JJ Kim, SH Seok, YJ Lee, SM Hong, SH An, SY Kim, YB Kim, JY Hwang, HJ Lee, HB Kim, DG Jeong, D Song, M Song, MS Park, KS Choi, JW Park, JY Seo, JW Yun, JS Shin, HY Lee, KT Nam, JK Seong
    Disease Models & Mechanisms, 2022-11-11;0(0):.
    Species: Hamster, Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  19. Essential role of IL-17 in acute exacerbation of pulmonary fibrosis induced by non-typeable Haemophilus influenzae
    Authors: S Chen, X Zhang, C Yang, S Wang, H Shen
    Theranostics, 2022-07-04;12(11):5125-5137.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. A conserved YAP/Notch/REST network controls the neuroendocrine cell fate in the lungs
    Authors: YT Shue, AP Drainas, NY Li, SM Pearsall, D Morgan, N Sinnott-Ar, SQ Hipkins, GL Coles, JS Lim, AE Oro, KL Simpson, C Dive, J Sage
    Nature Communications, 2022-05-16;13(1):2690.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. Heparan sulfate-dependent RAGE oligomerization is indispensable for pathophysiological functions of RAGE
    Authors: M Li, CY Ong, CJ Langouët-A, L Tan, A Verma, Y Yang, X Zhang, DK Shah, EP Schmidt, D Xu
    Elife, 2022-02-09;11(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  22. DCN released from ferroptotic cells ignites AGER-dependent immune responses
    Authors: J Liu, S Zhu, L Zeng, J Li, DJ Klionsky, G Kroemer, J Jiang, D Tang, R Kang
    Autophagy, 2021-12-29;0(0):1-14.
    Species: Mouse
    Sample Types: Cell Lysates, In Vivo
    Applications: Immunoprecipitation, Neutralization, Western Blot
  23. Differentiation of mouse fetal lung alveolar progenitors in serum-free organotypic cultures
    Authors: K Gkatzis, P Panza, S Peruzzo, DY Stainier
    Elife, 2021-09-29;10(0):.
    Species: Mouse
    Sample Types: Organoids
    Applications: IHC
  24. Overexpression of the receptor for advanced glycation end-products in the auditory cortex of rats with noise-induced hearing loss
    Authors: CH Lee, KW Kim, DH Lee, SM Lee, SY Kim
    Bmc Neuroscience, 2021-05-21;22(1):38.
    Species: Rat
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  25. Alveolar epithelial cell fate is maintained in a spatially restricted manner to promote lung regeneration after acute injury
    Authors: DC Liberti, MM Kremp, WA Liberti, IJ Penkala, S Li, S Zhou, EE Morrisey
    Cell Reports, 2021-05-11;35(6):109092.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  26. Dose-Dependent Effects of Resveratrol on Cisplatin-Induced Hearing Loss
    Authors: CH Lee, KW Kim, SM Lee, SY Kim
    International Journal of Molecular Sciences, 2020-12-24;22(1):.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  27. Regeneration of the pulmonary vascular endothelium after viral pneumonia requires COUP-TF2
    Authors: Gan Zhao, Aaron I. Weiner, Katherine M. Neupauer, Maria Fernanda de Mello Costa, Gargi Palashikar, Stephanie Adams-Tzivelekidis et al.
    Science Advances
  28. Human Lung Stem Cell-Based Alveolospheres Provide Insights into SARS-CoV-2-Mediated Interferon Responses and Pneumocyte Dysfunction
    Authors: H Katsura, V Sontake, A Tata, Y Kobayashi, CE Edwards, BE Heaton, A Konkimalla, T Asakura, Y Mikami, EJ Fritch, PJ Lee, NS Heaton, RC Boucher, SH Randell, RS Baric, PR Tata
    Cell Stem Cell, 2020-10-21;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  29. Gli1+ mesenchymal stromal cells form a pathological niche to promote airway progenitor metaplasia in the fibrotic lung
    Authors: M Cassandras, C Wang, J Kathiriya, T Tsukui, P Matatia, M Matthay, P Wolters, A Molofsky, D Sheppard, H Chapman, T Peng
    Nat Cell Biol, 2020-10-12;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  30. Angiocrine Sphingosine-1-Phosphate Activation of S1PR2-YAP Signaling Axis in Alveolar Type II Cells Is Essential for Lung Repair
    Authors: Q Chen, J Rehman, M Chan, P Fu, SM Dudek, V Natarajan, AB Malik, Y Liu
    Cell Rep, 2020-06-30;31(13):107828.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  31. TAZ is required for lung alveolar epithelial cell differentiation after injury
    Authors: T Sun, Z Huang, H Zhang, C Posner, G Jia, TR Ramalingam, M Xu, HD Brightbill, JG Egen, A Dey, JR Arron
    JCI Insight, 2019-06-18;5(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  32. IL-1 and TNF? Contribute to the Inflammatory Niche to Enhance Alveolar Regeneration
    Authors: H Katsura, Y Kobayashi, PR Tata, BLM Hogan
    Stem Cell Reports, 2019-03-28;0(0):.
    Species: Mouse
    Sample Types: Organoids
    Applications: IHC-P
  33. Pulmonary pericytes regulate lung morphogenesis
    Authors: K Kato, R Diéguez-Hu, DY Park, SP Hong, S Kato-Azuma, S Adams, M Stehling, B Trappmann, JL Wrana, GY Koh, RH Adams
    Nat Commun, 2018-06-22;9(1):2448.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  34. RAGE-specific single chain Fv for PET imaging of pancreatic cancer
    Authors: HY Kim, X Wang, R Kang, D Tang, BA Boone, HJ Zeh, MT Lotze, WB Edwards
    PLoS ONE, 2018-03-12;13(3):e0192821.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, ICC, IHC
  35. Therapeutic Effectiveness of Anti-RAGE Antibody Administration in a Rat Model of Crush Injury
    Authors: H Matsumoto, N Matsumoto, J Shimazaki, J Nakagawa, Y Imamura, K Yamakawa, T Yamada, M Ikeda, H Hiraike, H Ogura, T Shimazu
    Sci Rep, 2017-09-25;7(1):12255.
    Species: Rat
    Sample Types: Whole Tissue
    Applications: IHC
  36. Recruited Monocytes and Type 2 Immunity Promote Lung Regeneration following Pneumonectomy
    Authors: AJ Lechner, IH Driver, J Lee, CM Conroy, A Nagle, RM Locksley, JR Rock
    Cell Stem Cell, 2017-05-11;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  37. The development and plasticity of alveolar type 1 cells
    Authors: J Yang, BJ Hernandez, D Martinez A, O Narvaez de, L Vila-Ellis, H Akiyama, SE Evans, EJ Ostrin, J Chen
    Development, 2015-11-19;143(1):54-65.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  38. Two nested developmental waves demarcate a compartment boundary in the mouse lung.
    Authors: Alanis, Denise M, Chang, Daniel R, Akiyama, Haruhiko, Krasnow, Mark A, Chen, Jichao
    Nat Commun, 2014-05-29;5(0):3923.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  39. Alveolar progenitor and stem cells in lung development, renewal and cancer.
    Authors: Desai, Tushar J, Brownfield, Douglas, Krasnow, Mark A
    Nature, 2014-02-05;507(7491):190-4.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  40. Receptor for advanced glycation end products (RAGE) functions as receptor for specific sulfated glycosaminoglycans, and anti-RAGE antibody or sulfated glycosaminoglycans delivered in vivo inhibit pulmonary metastasis of tumor cells.
    Authors: Mizumoto S, Takahashi J, Sugahara K
    J. Biol. Chem., 2012-04-09;287(23):18985-94.
    Species: Mouse
    Sample Types: In Vivo, Whole Cells
    Applications: Neutralization
  41. Aldose reductase and AGE-RAGE pathways: central roles in the pathogenesis of vascular dysfunction in aging rats.
    Authors: Hallam KM, Li Q, Ananthakrishnan R, Kalea A, Zou YS, Vedantham S, Schmidt AM, Yan SF, Ramasamy R
    Aging Cell, 2010-08-15;9(5):776-84.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  42. Role of soluble receptor for advanced glycation end products on endotoxin-induced lung injury.
    Authors: Zhang H, Tasaka S, Shiraishi Y, Fukunaga K, Yamada W, Seki H, Ogawa Y, Miyamoto K, Nakano Y, Hasegawa N, Miyasho T, Maruyama I, Ishizaka A
    Am. J. Respir. Crit. Care Med., 2008-06-05;178(4):356-62.
    Species: Mouse
    Sample Types: BALF
    Applications: Western Blot
  43. Alpha-4/beta-1 and alpha-L/beta-2 integrins mediate cytokine induced lung leukocyte-epithelial adhesion and injury.
    Authors: Parmley LA, Elkins ND, Fini MA, Liu YE, Repine JE, Wright RM
    Br. J. Pharmacol., 2007-09-10;152(6):915-29.
    Species: Rat
    Sample Types: BALF
    Applications: Western Blot
  44. Low protein-induced intrauterine growth restriction as a risk factor for schizophrenia phenotype in a rat model: assessing the role of oxidative stress and neuroinflammation interaction
    Authors: Larissa Allgäuer, Jan-Harry Cabungcal, Catherine Yzydorczyk, Kim Quang Do, Daniella Dwir
    Translational Psychiatry
  45. delta Np63 drives dysplastic alveolar remodeling and restricts epithelial plasticity upon severe lung injury
    Authors: Aaron I. Weiner, Gan Zhao, Hanna M. Zayas, Nicolas P. Holcomb, Stephanie Adams-Tzivelekidis, Joanna Wong et al.
    Cell Reports
  46. Ager-CreERT2: A New Genetic Tool for Studying Lung Alveolar Development, Homeostasis, and Repair
    Authors: Mei-I Chung, Brigid L. M. Hogan
    American Journal of Respiratory Cell and Molecular Biology
  47. Nephrin loss is reduced by grape seed proanthocyanidins in the experimental diabetic nephropathy rat model
    Authors: Xianhua Li, Zhaoli Gao, Haiqing Gao, Baoying Li, Tao Peng, Bei Jiang et al.
    Molecular Medicine Reports
  48. Regeneration of the pulmonary vascular endothelium after viral pneumonia requires COUP-TF2
    Authors: Gan Zhao, Aaron I. Weiner, Katherine M. Neupauer, Maria Fernanda de Mello Costa, Gargi Palashikar, Stephanie Adams-Tzivelekidis et al.
    Science Advances
  49. Mouse models of lung-specific SARS-CoV-2 infection with moderate pathological traits
    Authors: Kim SH, Kim J, Jang JY et al.
    Frontiers in Immunology
  50. MMP9/RAGE pathway overactivation mediates redox dysregulation and neuroinflammation, leading to inhibitory/excitatory imbalance: a reverse translation study in schizophrenia patients
    Authors: Daniella Dwir, Basilio Giangreco, Lijing Xin, Liliane Tenenbaum, Jan-Harry Cabungcal, Pascal Steullet et al.
    Molecular Psychiatry
  51. Lung epithelial branching program antagonizes alveolar differentiation.
    Authors: Chang DR et al.
    Proc Natl Acad Sci U S A
  52. Multi-apical polarity of alveolar stem cells and their dynamics during lung development and regeneration
    Authors: Arvind Konkimalla, Satoshi Konishi, Yoshihiko Kobayashi, Preetish Kadur Lakshminarasimha Murthy, Lauren Macadlo, Ananya Mukherjee et al.
    iScience
  53. Persistent Pathology in Influenza-Infected Mouse Lungs
    Authors: Cindy M. Kanegai, Ying Xi, Matthew L. Donne, Jeffrey E. Gotts, Ian H. Driver, Gorica Amidzic et al.
    American Journal of Respiratory Cell and Molecular Biology
  54. Distinct Airway Epithelial Stem Cells Hide among Club Cells but Mobilize to Promote Alveolar Regeneration
    Authors: JJ Kathiriya, AN Brumwell, JR Jackson, X Tang, HA Chapman
    Cell Stem Cell, 2020-01-23;0(0):.
  55. Automated cell-type classification in intact tissues by single-cell molecular profiling
    Authors: Monica Nagendran, Daniel P Riordan, Pehr B Harbury, Tushar J Desai
    eLife

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Mouse/Rat RAGE Antibody
By Anonymous on 08/25/2021
Application: IF Sample Tested: Kidney tissue Species: Mouse