Mouse/Rat Thrombopoietin/Tpo Antibody Summary
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Cell Proliferation Induced by Thrombopoietin/Tpo and Neutralization by Mouse Thrombopoietin/Tpo Antibody. Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO) stimulates proliferation in the MO7e human megakaryocytic leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse Thrombopoietin/Tpo (3 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse/Rat Thrombopoietin/Tpo Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-488-NA). The ND50 is typically 0.1-0.3 µg/mL.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Thrombopoietin/Tpo
Thrombopoietin (Tpo), is a key regulator of megakaryocytopoiesis and thrombopoiesis. It is principally produced in the liver and is bound and internalized by the receptor Tpo R/c-mpl. Defects in the Tpo-Tpo R signaling pathway are associated with a variety of platelet disorders (1‑3). The 356 amino acid (aa) mouse Tpo precursor is cleaved to yield the 335 aa mature protein. Mature mouse Tpo shares 71% and 81% aa sequence homology with human and rat Tpo, respectively. It is an 80‑85 kDa protein that consists of an N-terminal domain with homology to Erythropoietin (Epo) and a C‑terminal domain that contains multiple N-linked and O-linked glycosylation sites (4, 5). Tissue specific alternate splicing of mouse Tpo generates multiple isoforms with internal deletions, insertions, and/or C‑terminal substitutions (6). Tpo promotes the differentiation, proliferation, and maturation of MK and their progenitors (4, 5, 7). Several other cytokines can promote these functions as well but only in cooperation with Tpo (8, 9). Notably, IL-3 independently induces MK development, although its effects are restricted to early in the MK lineage (8, 9). Tpo additionally promotes platelet production, aggregation, ECM adhesion, and activation (10 - 13). It is cleaved by platelet-derived thrombin following Arg191 within the C‑terminal domain and subsequently at other sites upon extended digestion (14). Full length Tpo and shorter forms circulate in the plasma (4, 5). The C‑terminal domain is not required for binding to Tpo R or inducing MK growth and differentiation (5). Aside from its hematopoietic effects, Tpo is expressed in the brain where it promotes the apoptosis of hypoxia-sensitized neurons and inhibits neuronal differentiation by blocking NGF-induced signaling (15, 16).
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Deutsch, V.R. and A. Tomer (2006)
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Kaushansky, K. (2005) J. Clin. Invest. 115:3339.
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Li, J. et al. (1999)
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Bartley, T.D. et al. (1994) Cell 77:1117.
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de Sauvage, F.J. et al. (1994) Nature 369:533.
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Marcucci, R. and M. Romano (2008) Biochim. Biophys. Acta 1782:427.
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Kaushansky, K. et al. (1994) Nature 369:568.
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Kaushansky, K. et al. (1995) Proc. Natl. Acad. Sci. 92:3234.
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Broudy, V.C. et al. (1995) Blood 85:1719.
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Lok, S.I. et al. (1994) Nature 369:565.
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Chen, J. et al. (1995) Blood 86:4054.
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Oda, A. et al. (1996) Blood 87:4664.
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Van Os, E. et al. (2003) Br. J. Haematol. 121:482.
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Kato, T. et al. (1997) Proc. Natl. Acad. Sci. 94:4669.
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Ehrenreich, H. et al. (2005) Proc. Natl. Acad. Sci. 102:862.
- Samoylenko, A. et al. (2008) Cell. Signal. 20:154.
Product Datasheets
Citations for Mouse/Rat Thrombopoietin/Tpo Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 4
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Adipocyte Fatty Acid Transfer Supports Megakaryocyte Maturation
Authors: C Valet, A Batut, A Vauclard, A Dortignac, M Bellio, B Payrastre, P Valet, S Severin
Cell Rep, 2020-07-07;32(1):107875.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Multicolor quantitative confocal imaging cytometry
Authors: DL Coutu, KD Kokkaliari, L Kunz, T Schroeder
Nat. Methods, 2017-11-13;15(1):39-46.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Thrombopoietin contributes to the formation and the maintenance of hematopoietic progenitor-containing cell clusters in the aorta-gonad-mesonephros region
Authors: K Harada, I Nobuhisa, M Anani, K Saito, T Taga
Cytokine, 2017-02-21;95(0):35-42.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization -
A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells.
Authors: Shih CC, Hu MC, Hu J
Blood, 2000-03-15;95(6):1957-66.
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization
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