Mouse/Rat TREM-1 Quantikine ELISA Kit Summary
Product Summary
Recovery
The recovery of TREM-1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Mouse Cell Culture Supernates (n=5) | 98 | 87-109 |
| Mouse EDTA Plasma (n=6) | 99 | 90-108 |
| Mouse Heparin Plasma (n=5) | 101 | 89-110 |
| Mouse Serum (n=5) | 98 | 81-106 |
| Mouse Tissue Homogenates (n=2) | 100 | 91-104 |
| Rat Cell Culture Supernates (n=5) | 97 | 90-109 |
| Rat EDTA Plasma (n=5) | 100 | 85-112 |
| Rat Heparin Plasma (n=5) | 99 | 85-113 |
| Rat Serum (n=4) | 107 | 84-120 |
| Rat Tissue Homogenates (n=2) | 99 | 95-103 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: TREM-1
The TREM family of receptors regulates the activity of various cell types of the immune system including neutrophils, monocyte/macrophages, microglia, and dendritic cells. The receptors are members of the immunoglobulin superfamily, characterized structurally by a single V-set immunoglobulin (Ig) domain and a short cytoplasmic tail. TREMs associate with the DAP12 signal adapter, an immunoreceptor tyrosine-based activation motif- (ITAM)-containing protein that is phosphorylated and transduces signals when activated.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Mouse/Rat TREM-1 Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Single-cell RNA sequencing identifies an Il1rn+/Trem1+ macrophage subpopulation as a cellular target for mitigating the progression of thoracic aortic aneurysm and dissection
Authors: X Liu, W Chen, G Zhu, H Yang, W Li, M Luo, C Shu, Z Zhou
Cell Discovery, 2022-02-08;8(1):11.
Species: Mouse
Sample Types: Plasma
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Targeted endothelial gene deletion of Triggering Receptor Expressed on Myeloid cells-1 protects mice during septic shock
Authors: L Jolly, K Carrasco, M Derive, J Lemarié, A Boufenzer, S Gibot
Cardiovasc. Res., 2018-05-01;0(0):.
Species: Mouse
Sample Types: Plasma
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TREM-1 promotes survival during septic shock in mice.
Authors: Gibot S, Massin F, Marcou M, Taylor V, Stidwill R, Wilson P, Singer M, Bellingan G
Eur. J. Immunol., 2007-02-01;37(2):456-66.
Species: Mouse
Sample Types: Tissue Homogenates
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