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Mouse Reelin Antibody

Catalog #: AF3820
22 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3820
AF3820-SP
Reelin in Mouse Embryo.
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Product Details
Citations (22)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Reelin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Reelin in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Reelin
Ala27-Met862
Accession # Q60841
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Reelin
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Reelin antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr). View Larger

Reelin in Mouse Embryo. Reelin was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Goat Anti-Mouse Reelin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3820) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Reelin

Reelin is a 400 kDa, extracellular matrix glycoprotein secreted by several neurons. It is a serine protease that degrades fibronectin and laminin. Reelin also binds lipoprotein receptor superfamily members APOER2 and VLDLR which transduce signals important for neuronal positioning during brain development and synaptic plasticity in the adult brain. In vivo, Reelin undergoes proteolytic processing at two sites, generating three Reelin fragments. The N-terminal domain binds integrin alpha 3/ beta 1, leading to a disruption of neuronal glial cell interactions and inhibition of neuronal migration. Mouse Reelin shares 94% and 96% amino acid identity with human and rat Reelin, respectively in the region immunized.

Entrez Gene IDs
5649 (Human); 19699 (Mouse); 24718 (Rat)
Alternate Names
EC 3.4.21; EC 3.4.21.-; Reeler; Reelin; RELN; RL; RLPRO1598

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Citations for Mouse Reelin Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. The Secreted Glycoprotein Reelin Suppresses the Proliferation and Regulates the Distribution of Oligodendrocyte Progenitor Cells in the Embryonic Neocortex
    Authors: Himari Ogino, Tsuzumi Nakajima, Yuki Hirota, Kohki Toriuchi, Mineyoshi Aoyama, Kazunori Nakajima et al.
    The Journal of Neuroscience
  2. Prox1 Regulates the Subtype-Specific Development of Caudal Ganglionic Eminence-Derived GABAergic Cortical Interneurons
    Authors: Goichi Miyoshi, Allison Young, Timothy Petros, Theofanis Karayannis, Melissa McKenzie Chang, Alfonso Lavado et al.
    The Journal of Neuroscience
  3. Reelin dorsal horn neurons co‐express Lmx1b and are mispositioned in disabled‐1 mutant mice
    Authors: Griselda M. Yvone, Carmine L. Chavez‐Martinez, Amanda R. Nguyen, Deborah J. Wang, Patricia E. Phelps
    European Journal of Neuroscience
  4. Different activity patterns control various stages of Reelin synthesis in the developing neocortex
    Authors: Kira Engeroff, Davide Warm, Stefan Bittner, Oriane Blanquie
    Cerebral Cortex
  5. VLDLR is not essential for reelin-induced neuronal aggregation but suppresses neuronal invasion into the marginal zone
    Authors: Yuki Hirota, Kazunori Nakajima
    Development
  6. Activation of the PI3K/AKT/mTOR Pathway in Cajal-Retzius Cells Leads to Their Survival and Increases Susceptibility to Kainate-Induced Seizures
    Authors: N Ramezanido, DE Ouardi, M Le, S Moriceau, M Ahmadi, D Elena, D Rolland, P Bun, G Le Pen, G Canaud, N Bahi-Buiss, N Rouach, R Piskorowsk, A Pierani, P Billuart
    International Journal of Molecular Sciences, 2023-03-11;24(6):.
  7. Deficiency of Stabilin-1 in the Context of Hepatic Melanoma Metastasis
    Authors: Wohlfeil, SA;Olsavszky, A;Irkens, AL;Häfele, V;Dietsch, B;Straub, N;Goerdt, S;Géraud, C;
    Cancers
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. Single-cell long-read sequencing in human cerebral organoids uncovers cell-type-specific and autism-associated exons
    Authors: Yang, Y;Yang, R;Kang, B;Qian, S;He, X;Zhang, X;
    Cell reports
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Aberrant survival of hippocampal Cajal-Retzius cells leads to memory deficits, gamma rhythmopathies and susceptibility to seizures in adult mice
    Authors: M Riva, S Moriceau, A Morabito, E Dossi, C Sanchez-Be, P Azzam, A Navas-Oliv, B Gal, F Dori, E Cid, F Ledonne, S David, F Trovero, M Bartolomuc, E Coppola, N Rebola, A Depaulis, N Rouach, LM de la Prid, F Oury, A Pierani
    Nature Communications, 2023-03-18;14(1):1531.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Cxcr4 and Ackr3 regulate allocation of caudal ganglionic eminence-derived interneurons to superficial cortical layers
    Authors: S Venkataram, F Saaber, P Abe, D Schütz, PA Kumar, R Stumm
    Cell Reports, 2022-08-02;40(5):111157.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  11. Lymphatics act as a signaling hub to regulate intestinal stem cell activity
    Authors: RE Niec, T Chu, M Schernthan, S Gur-Cohen, L Hidalgo, HA Pasolli, KA Luckett, Z Wang, SR Bhalla, F Cambuli, RP Kataru, K Ganesh, BJ Mehrara, D Pe'er, E Fuchs
    Cell Stem Cell, 2022-06-20;29(7):1067-1082.e18.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  12. Dynamic cell contacts between periportal mesenchyme and ductal epithelium act as a rheostat for liver cell proliferation
    Authors: L Cordero-Es, AM Dowbaj, TN Kohler, B Strauss, O Sarlidou, G Belenguer, C Pacini, NP Martins, R Dobie, JR Wilson-Kan, R Butler, N Prior, P Serup, F Jug, NC Henderson, F Hollfelder, M Huch
    Cell Stem Cell, 2021-08-02;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  13. Developmental, cellular, and behavioral phenotypes in a mouse model of congenital hypoplasia of the dentate gyrus
    Authors: A Rattner, CE Terrillion, C Jou, T Kleven, SF Hu, J Williams, Z Hou, M Aggarwal, S Mori, G Shin, LA Goff, MP Witter, M Pletnikov, AA Fenton, J Nathans
    Elife, 2020-10-21;9(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  14. Chronic Corticosterone Elevation Suppresses Adult Hippocampal Neurogenesis by Hyperphosphorylating Huntingtin
    Authors: F Agasse, I Mendez-Dav, W Christalle, R Carpentier, BY Braz, DJ David, F Saudou, S Humbert
    Cell Rep, 2020-07-07;32(1):107865.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  15. Physiological significance of proteolytic processing of Reelin revealed by cleavage-resistant Reelin knock-in mice
    Authors: E Okugawa, H Ogino, T Shigenobu, Y Yamakage, H Tsuiji, H Oishi, T Kohno, M Hattori
    Sci Rep, 2020-03-11;10(1):4471.
    Species: Mouse
    Sample Types: Cell lysta
    Applications: Western Blot
  16. Loss-of-huntingtin in medial and lateral ganglionic lineages differentially disrupts regional interneuron and projection neuron subtypes and promotes Huntington's disease-associated behavioral, cellular and pathological hallmarks
    Authors: MF Mehler, JR Petronglo, EE Arteaga-Br, M Gulinello, ML Winchester, N Pichamoort, SK Young, CD DeJesus, H Ishtiaq, S Gokhan, AE Molero
    J. Neurosci., 2019-01-09;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Secreted Metalloproteinase ADAMTS-3 Inactivates Reelin
    Authors: H Ogino, A Hisanaga, T Kohno, Y Kondo, K Okumura, T Kamei, T Sato, H Asahara, H Tsuiji, M Fukata, M Hattori
    J. Neurosci, 2017-02-17;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Importance of Reelin C-terminal region in the development and maintenance of the postnatal cerebral cortex and its regulation by specific proteolysis.
    Authors: Kohno T, Honda T, Kubo K, Nakano Y, Tsuchiya A, Murakami T, Banno H, Nakajima K, Hattori M
    J Neurosci, 2015-03-18;35(11):4776-87.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  19. Cleavage within Reelin repeat 3 regulates the duration and range of the signaling activity of Reelin protein.
    Authors: Koie, Mari, Okumura, Kyoko, Hisanaga, Arisa, Kamei, Takana, Sasaki, Kazutomo, Deng, Mengyan, Baba, Atsushi, Kohno, Takao, Hattori, Mitsuhar
    J Biol Chem, 2014-03-18;289(18):12922-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  20. Smooth muscle-endothelial cell communication activates Reelin signaling and regulates lymphatic vessel formation.
    J. Cell Biol., 2012-06-04;197(6):837-49.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  21. Single-Cell Transcriptomics Uncovers Zonation of Function in the Mesenchyme during Liver Fibrosis
    Authors: Ross Dobie, John R. Wilson-Kanamori, Beth E.P. Henderson, James R. Smith, Kylie P. Matchett, Jordan R. Portman et al.
    Cell Reports
  22. Impaired Interneuron Development in a Novel Model of Neonatal Brain Injury
    Authors: Helene Lacaille, Claire-Marie Vacher, Dana Bakalar, Jiaqi J. O'Reilly, Jacquelyn Salzbank, Anna A. Penn
    eNeuro

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