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Mouse RELM beta Antibody

Catalog #: AF2730
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2730
AF2730-SP
Detection of Mouse RELM beta by Western Blot.
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Citations (1)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse RELM beta Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse RELM beta in direct ELISAs and Western blots. In direct ELISAs, approximately 40% cross-reactivity with recombinant rat RELM gamma is observed and 10% cross-reactivity with recombinant mouse (rm) Resistin and rmRELM alpha is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse RELM beta
Gly21-Ala105
Accession # Q99P86
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse RELM beta antibody by Western Blot. View Larger

Detection of Mouse RELM beta by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse RELM beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2730) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for RELM beta at approximately 19 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RELM beta

RELM beta, also known as FIZZ2 (found in inflammatory zone 2) is a 9 kDa secreted, cysteine-rich protein belonging to the RELM family. By analogy to Resistin, RELM beta is expected to exist in two distinct multimeric structures corresponding to hexamers and trimers. RELM beta is synthesized by intestinal goblet cells and is secreted into the intestinal lumen. Mouse RELM beta shares 61% and 77% amino acid (aa) sequence identity with human and rat RELM beta, respectively. It also shares 52%, 71% and 46% aa sequence identity with mouse RELM alpha, RELM gamma and Resistin, respectively.

Long Name
Resistin-like Molecule beta
Entrez Gene IDs
84666 (Human); 57263 (Mouse)
Alternate Names
C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich proteinprecursor 2; CCRG; Colon and small intestine-specific cysteine-rich protein; Colon carcinoma-related gene protein; cysteine-rich secreted A12-alpha-like protein 1; Cysteine-rich secreted protein A12-alpha-like 1; Cysteine-rich secreted protein FIZZ2; FIZZ1; FIZZ2RELM-beta; found in inflammatory zone 1; HXCP2XCP2; RELM beta; RELMb; RELMbeta; resistin like beta; resistin-like beta; RETNL2

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Citation for Mouse RELM beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Lactobacillus salivarius reverse antibiotic-induced lung defense impairment in a ventilator model
    Authors: TB Tsay, MC Yang, WH Chang, PH Chen, LW Chen
    J Transl Med, 2018-08-13;16(1):225.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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