Mouse S100A10 Antibody Summary
Met1-Lys97
Accession # P08207
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Mouse S100A10 by Western Blot. Western blot shows lysates of mouse lung tissue and MEF mouse embryonic feeder cells. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse S100A10 Monoclonal Antibody (Catalog # MAB2377) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for S100A10 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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S100A10 in TK‑1 Mouse T Cell Line. S100A10 was detected in immersion fixed TK‑1 mouse T cell lymphoma cell line using Rat Anti-Mouse S100A10 Monoclonal Antibody (Catalog # MAB2377) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.
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Detection of S100A10 in Raw264 Mouse Cell Line by Flow Cytometry. Raw264 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse S100A10 Monoclonal Antibody (Catalog # MAB2377, filled histogram) or Rat IgG2A Isotype Control (MAB006, open histogram) followed by PE-conjugated Anti-Rat IgG Secondary Antibody (F0105B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (FC012). Staining was performed using our Staining Intracellular Proteins protocol.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100A10
S100A10, also called p11, is a 97 amino acid member of the S100 family of EF hand proteins, but does not bind calcium. It is primarily found, either within or on the surface of mast cells, as a complex of two annexin A2 subunits with two S100A10 subunits. Extracellular S100A10 is a plasminogen receptor important for plasmin production and cellular invasiveness. Intracellular S100A10 may target ligands to the endoplasmic reticulum. Mouse S100A10 shows 92% amino acid identity with human S100A10.
Product Datasheets
Citations for Mouse S100A10 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 2
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PSA-NCAM colocalized with cholecystokinin-expressing cells in the hippocampus is involved in mediating antidepressant efficacy
Authors: J Yamada, C Sato, K Konno, M Watanabe, S Jinno
J. Neurosci., 2019-12-04;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Annexin2 coating the surface of enlargeosomes is needed for their regulated exocytosis.
Authors: Lorusso A, Covino C, Priori G, Bachi A, Meldolesi J, Chieregatti E
EMBO J., 2006-11-02;25(23):5443-56.
Species: Rat
Sample Types: Whole Cells
Applications: ICC
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