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Mouse S100A10 Antibody

Catalog #: AF2377
22 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF2377
AF2377-SP
Detection of Mouse S100A10 by Western Blot.
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Product Details
Citations (22)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse S100A10 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse S100A10 in direct ELISAs and Western blots. In these formats, approximately 5% cross-reactivity with recombinant human (rh) S100A10 is observed and less than 1% cross-reactivity with rhS100B and recombinant mouse S100A9 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse S100A10
Pro2-Lys97
Accession # P08207
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse S100A10 antibody by Western Blot. View Larger

Detection of Mouse S100A10 by Western Blot. Western blot shows lysates of mouse lung and MEF mouse embryonic feeder cells. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for S100A10 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunohistochemistry S100A10 antibody in Mouse Kidney by Immunohistochemistry (IHC-Fr). View Larger

S100A10 in Mouse Kidney. S100A10 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western Detection of Mouse S100A10 antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Mouse S100A10 by Simple WesternTM. Simple Western lane view shows lysates of mouse lung tissue and MEF mouse embryonic feeder cells, loaded at 0.2 mg/mL. A specific band was detected for S100A10 at approximately 11 kDa (as indicated) using 1 µg/mL of Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S100A10

S100A10 is a member of the S100 family of EF hand calcium-binding proteins. It is primarily found in mast cells as well as heterotetrameric complexes (two annexin A2 subunits and two S100 A10 subunits) that are present intracellularly at the plasma membrane or on the extracellular cell surface. Extracellular S100A10 is a key plasminogen receptor that plays an important role in cellular plasmin production and cellular invasiveness.

Long Name
S100 Calcium Binding Protein
Entrez Gene IDs
6281 (Human); 20194 (Mouse)
Alternate Names
annexin II ligand, calpactin I, light polypeptide; ANX2L; ANX2LG; CAL1LGP11,42C; Calpactin I light chain; Calpactin-1 light chain; Cellular ligand of annexin II; CLP11Ca[1]; MGC111133; p10 protein; p10; P11; protein S100-A10; S100 calcium binding protein A10 (annexin II ligand, calpactin I, lightpolypeptide (p11)); S100 calcium binding protein A10; S100 calcium-binding protein A10 (annexin II ligand, calpactin I, lightpolypeptide (p11)); S100 calcium-binding protein A10; S100A10

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Citations for Mouse S100A10 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. Sp1-regulated expression of p11 contributes to motor neuron degeneration by membrane insertion of TASK1
    Authors: Victoria García-Morales, Guillermo Rodríguez-Bey, Laura Gómez-Pérez, Germán Domínguez-Vías, David González-Forero, Federico Portillo et al.
    Nature Communications
  2. Annexin A2 supports pulmonary microvascular integrity by linking vascular endothelial cadherin and protein tyrosine phosphatases
    Authors: Luo M, Flood EC, Almeida D et al.
    J. Exp. Med.
  3. Blocking C3d+/GFAP+ A1 Astrocyte Conversion with Semaglutide Attenuates Blood-Brain Barrier Disruption in Mice after Ischemic Stroke
    Authors: Qi Zhang, Chang Liu, Rubing Shi, Shiyi Zhou, Huimin Shan, Lidong Deng et al.
    Aging and disease
  4. AP-1 controls the p11-dependent antidepressant response
    Authors: RU Chottekala, S Kalik, J Gresack, A Ayala, M Gao, W Wang, S Meller, A Aly, A Schaefer, P Greengard
    Mol. Psychiatry, 2020-05-21;0(0):.
  5. Cell- and region-specific expression of depression-related protein p11 (S100a10) in the brain
    Authors: Ana Milosevic, Thomas Liebmann, Margarete Knudsen, Nicoletta Schintu, Per Svenningsson, Paul Greengard
    Journal of Comparative Neurology
  6. Systemic LPS Administration Stimulates the Activation of Non-Neuronal Cells in an Experimental Model of Spinal Muscular Atrophy
    Authors: Karafoulidou, E;Kesidou, E;Theotokis, P;Konstantinou, C;Nella, MK;Michailidou, I;Touloumi, O;Polyzoidou, E;Salamotas, I;Einstein, O;Chatzisotiriou, A;Boziki, MK;Grigoriadis, N;
    Cells
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  7. Active secretion of IL-33 from astrocytes is dependent on TMED10 and promotes central nervous system homeostasis
    Authors: Jiao, M;Wang, C;Tang, X;Dai, C;Zhang, N;Fan, A;Qian, Z;Liu, S;Zhang, F;Li, B;Xu, Y;Tan, Z;Gong, F;Lu, Y;Zheng, F;
    Brain, behavior, and immunity
    Species: Mouse, Transgenic Mouse
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Western Blot
  8. Effect of rottlerin on astrocyte phenotype polarization after trimethyltin insult in the dentate gyrus of mice
    Authors: Y Hwang, HC Kim, EJ Shin
    Journal of Neuroinflammation, 2022-06-11;19(1):142.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  9. Inhibition of the NLRP3-inflammasome prevents cognitive deficits in experimental autoimmune encephalomyelitis mice via the alteration of astrocyte phenotype
    Authors: B Hou, Y Zhang, P Liang, Y He, B Peng, W Liu, S Han, J Yin, X He
    Cell Death Dis, 2020-05-15;11(5):377.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. Neuroprotective efficacy of different levels of high-frequency repetitive transcranial magnetic stimulation in mice with CUMS-induced depression: Involvement of the p11/BDNF/Homer1a signaling pathway
    Authors: C Zuo, H Cao, F Ding, J Zhao, Y Huang, G Li, S Huang, H Jiang, Y Jiang, F Wang
    J Psychiatr Res, 2020-04-05;125(0):152-163.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  11. PSA-NCAM colocalized with cholecystokinin-expressing cells in the hippocampus is involved in mediating antidepressant efficacy
    Authors: J Yamada, C Sato, K Konno, M Watanabe, S Jinno
    J. Neurosci., 2019-12-04;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Mapping the physiological and molecular markers of stress and SSRI antidepressant treatment in S100a10 corticostriatal neurons
    Authors: D Sargin, RU Chottekala, KE Perit, V Yao, D Chu, DW Sparks, S Kalik, SK Power, OG Troyanskay, EF Schmidt, P Greengard, EK Lambe
    Mol. Psychiatry, 2019-08-20;25(5):1112-1129.
    Species: Mouse
    Sample Types: WholeTissue
    Applications: IHC
  13. P11 Loss-of-Function is Associated with Decreased Cell Proliferation and Neurobehavioral Disorders in Mice
    Authors: G Liu, Y Wang, W Zheng, H Cheng, R Zhou
    Int. J. Biol. Sci., 2019-05-20;15(7):1383-1395.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Ahnak scaffolds p11/Anxa2 complex and L-type voltage-gated calcium channel and modulates depressive behavior
    Authors: J Jin, DL Bhatti, KW Lee, L Medrihan, J Cheng, J Wei, P Zhong, Z Yan, C Kooiker, C Song, JH Ahn, GJ Obermair, A Lee, J Gresack, P Greengard, Y Kim
    Mol. Psychiatry, 2019-02-13;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  15. Gestational stress in mouse dams negatively affects gestation and postpartum hippocampal BDNF and P11 protein levels
    Authors: T Vanmierlo, J De Vry, E Nelissen, A Sierksma, N Roumans, HWM Steinbusch, LP Wennogle, D van den Ho, J Prickaerts
    Mol. Cell. Neurosci., 2018-02-28;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-F
  16. Molecular codes for cell type specification in Brn3 retinal ganglion cells
    Authors: S Sajgo, MG Ghinia, M Brooks, F Kretschmer, K Chuang, S Hiriyanna, Z Wu, O Popescu, TC Badea
    Proc. Natl. Acad. Sci. U.S.A., 2017-05-02;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. Identification of the cortical neurons that mediate antidepressant responses.
    Authors: Schmidt EF, Warner-Schmidt JL, Otopalik BG, Pickett SB, Greengard P, Heintz N
    Cell, 2012-05-25;149(5):1152-63.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  18. Characterization of S100A11, a suppressive factor of fertilization, in the mouse female reproductive tract.
    Authors: Hanaue M, Miwa N, Uebi T, Fukuda Y, Katagiri Y, Takamatsu K
    Mol. Reprod. Dev., 2011-01-20;78(2):91-103.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  19. Role of p11 in cellular and behavioral effects of 5-HT4 receptor stimulation.
    Authors: Warner-Schmidt JL, Flajolet M, Maller A, Chen EY, Qi H, Svenningsson P, Greengard P
    J. Neurosci., 2009-02-11;29(6):1937-46.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  20. p11 modulates calcium handling through 5-HT4R pathway in rat ventricular cardiomyocytes
    Authors: Pierre Meschin, Marie Demion, Olivier Cazorla, Amanda Finan, Jérôme Thireau, Sylvain Richard et al.
    Cell Calcium
  21. Reversal of Depressed Behaviors in Mice by p11 Gene Therapy in the Nucleus Accumbens
    Authors: Brian Alexander, Jennifer Warner-Schmidt, Therese Eriksson, Carol Tamminga, Margarita Arango-Lievano, Margarita Arango-Llievano et al.
    Science Translational Medicine
  22. p11 in Cholinergic Interneurons of the Nucleus Accumbens Is Essential for Dopamine Responses to Rewarding Stimuli
    Authors: Y. Hanada, Y. Kawahara, Y. N. Ohnishi, T. Shuto, M. Kuroiwa, N. Sotogaku et al.
    eNeuro

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