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Mouse S1P1/EDG-1 Antibody

Catalog #: MAB7089
28 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB7089
MAB7089-SP
Detection of S1P1/EDG‑1 in HEK293 Human Cell Line Transfected With S1P1/EDG‑1 by Flow Cytometry.
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Product Details
Citations (28)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse S1P1/EDG-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse S1P1/EDG‑1 peptide in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 713412
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse S1P1/EDG‑1 synthetic peptide (T4-H28)
Accession # O08530
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of S1P<SUB>1</SUB>/EDG-1 antibody in HEK293 Human Cell Line Transfected With S1P<SUB>1</SUB>/EDG-1 antibody by Flow Cytometry. View Larger

Detection of S1P1/EDG‑1 in HEK293 Human Cell Line Transfected With S1P1/EDG‑1 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG-1 and GFP was stained with (A) Rat Anti-Mouse S1P1/EDG-1 Monoclonal Antibody (Catalog # MAB7089) or (B) Rat IgG2A isotype control antibody (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of S1P1/EDG-1 antibody in Mouse Thymocytes antibody by Flow Cytometry. View Larger

Detection of S1P1/EDG‑1 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rat Anti-Mouse S1P1/EDG-1 Monoclonal Antibody (Catalog # MAB7089) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Immunohistochemistry S1P<SUB>1</SUB>/EDG-1 antibody in Mouse Heart by Immunohistochemistry (IHC-Fr). View Larger

S1P1/EDG‑1 in Mouse Heart. S1P1/EDG-1 was detected in perfusion fixed frozen sections of mouse heart using Rat Anti-Mouse S1P1/EDG-1 Monoclonal Antibody (Catalog # MAB7089) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to endothelial cells in capillaries. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: S1P1/EDG-1

S1P1 (sphingosine 1-phosphate receptor-1), also known as EDG-1 (endothelial differentiation, G-protein coupled receptor-1) or S1PR1 (sphingosine-1-phosphate receptor 1) is a widely expressed, 37-40 kDa, G protein coupled receptor within the S1P subfamily of the EDG family. S1P1 is one of five receptors for the bioactive lipid S1P and mediates most of S1P effects on angiogenesis, vascular maturation, and cell migration, especially T cell egress from lymphoid organs. Human and mouse S1P1 share 84% amino acid identity within the N-terminal extracellular portion used as an immunogen.

Long Name
Endothelial Differentiation Gene 1
Entrez Gene IDs
1901 (Human); 13609 (Mouse)
Alternate Names
CD363 antigen; CD363; CHEDG1; D1S3362; EDG1; EDG-1; EDG1edg-1; Endothelial differentiation G-protein coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; FLJ58121; S1P receptor 1; S1P receptor Edg-1; S1P1; S1P1ECGF1; S1PR1; sphingosine 1-phosphate receptor 1; sphingosine 1-phosphate receptor EDG1; Sphingosine 1-phosphate receptor Edg-1; sphingosine-1-phosphate receptor 1

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Citations for Mouse S1P1/EDG-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

28 Citations: Showing 1 - 10
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  1. ILC2s navigate tissue redistribution during infection using stage-specific S1P receptors
    Authors: Ito, T;Ishida, Y;Zhang, Y;Guichard, V;Zhang, W;Han, R;Guckian, K;Chun, J;Que, J;Smith, A;Urban, JF;Huang, Y;
    bioRxiv : the preprint server for biology
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. PTEN? directs developmental and metabolic signaling for innate-like T cell fate and tissue homeostasis
    Authors: DB Blanco, NM Chapman, JL Raynor, C Xu, W Su, A Kc, W Li, SA Lim, S Schattgen, H Shi, I Risch, Y Sun, Y Dhungana, Y Kim, J Wei, S Rankin, G Neale, PG Thomas, K Yang, H Chi
    Nature Cell Biology, 2022-10-27;24(11):1642-1654.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. The transcription factor LRF promotes integrin beta7 expression by and gut homing of CD8alphaalpha+ intraepithelial lymphocyte precursors
    Authors: J Nie, AC Carpenter, LB Chopp, T Chen, M Balmaceno-, T Ciucci, Q Xiao, MC Kelly, DB McGavern, Y Belkaid, R Bosselut
    Nature Immunology, 2022-03-30;23(4):594-604.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. ETS2 repressor factor (ERF) is involved in T lymphocyte maturation acting as regulator of thymocyte lineage commitment
    Authors: S Tsiomita, EM Liveri, P Vardaka, A Vogiatzi, A Skiadaresi, G Saridis, I Tsigkas, TM Michaelidi, G Mavrothala, G Thyphronit
    Journal of leukocyte biology, 2022-03-08;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Glycogen synthase kinase 3 drives thymocyte egress by suppressing beta-catenin activation of Akt
    Authors: C Liu, L Ma, Y Wang, J Zhao, P Chen, X Chen, Y Wang, Y Hu, Y Liu, X Jia, Z Yang, X Yin, J Wu, S Wu, H Zheng, X Ma, X Sun, Y He, L Lin, Y Fu, K Liao, X Zhou, S Jiang, G Fu, J Tang, W Han, XL Chen, W Fan, Y Hong, J Han, X Huang, BA Li, N Xiao, C Xiao, G Fu, WH Liu
    Science Advances, 2021-10-08;7(41):eabg6262.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. SPNS2 enables T�cell egress from lymph nodes during an immune response
    Authors: M Okuniewska, V Fang, A Baeyens, V Raghavan, JY Lee, DR Littman, SR Schwab
    Cell Reports, 2021-07-13;36(2):109368.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Monocyte-derived S1P in the lymph node regulates immune responses
    Authors: A Baeyens, S Bracero, VS Chaluvadi, A Khodadadi-, M Cammer, SR Schwab
    Nature, 2021-03-03;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Regulatory T Cell Stability and Migration Are Dependent on mTOR
    Authors: R Vallion, J Divoux, S Glauzy, E Ronin, Y Lombardi, M Lubrano di, S Grégoire, I Nemazanyy, A Durand, D Fradin, B Lucas, BL Salomon
    J. Immunol., 2020-08-24;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. S1PR1 regulates the quiescence of lymphatic vessels by inhibiting laminar shear stress-dependent VEGF-C signaling
    Authors: X Geng, K Yanagida, RG Akwii, D Choi, L Chen, Y Ho, B Cha, MR Mahamud, K Berman de, H Ichise, H Chen, J Wythe, CM Mikelis, T Hla, RS Srinivasan
    JCI Insight, 2020-07-23;0(0):.
    Species: Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. TBL1XR1 Mutations Drive Extranodal Lymphoma by Inducing a Pro-tumorigenic Memory Fate.
    Authors: Leandro V, Matt T, Andrew Z et al.
    Cell.
  11. Transforming Growth Factor-beta signaling in alphabeta thymocytes promotes negative selection
    Authors: MJ McCarron, M Irla, A Sergé, SM Soudja, JC Marie
    Nat Commun, 2019-12-19;10(1):5690.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. Amiselimod (MT-1303), a novel sphingosine 1-phosphate receptor-1 functional antagonist, inhibits progress of chronic colitis induced by transfer of CD4+CD45RBhigh�T cells
    Authors: K Shimano, Y Maeda, H Kataoka, M Murase, S Mochizuki, H Utsumi, K Oshita, K Sugahara
    PLoS ONE, 2019-12-05;14(12):e0226154.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Glycolipid Stimulation of Invariant NKT Cells Expands a Unique Tissue-Resident Population of Precursors to Mature NK Cells Endowed with Oncolytic and Antimetastatic Properties
    Authors: J Choi, PT Rudak, S Lesage, SMM Haeryfar
    J. Immunol., 2019-08-28;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Complex and Multilayered Role of IL-21 Signaling during Thymic Development
    Authors: MM Moretto, S Hwang, K Chen, IA Khan
    J. Immunol., 2019-07-24;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Endothelial cells act as gatekeepers for LT beta R-dependent thymocyte emigration
    Authors: Kieran D. James, Emilie J. Cosway, Beth Lucas, Andrea J. White, Sonia M. Parnell, Manuela Carvalho-Gaspar et al.
    Journal of Experimental Medicine
  16. A Coronin 1-Dependent Decision Switch in Juvenile Mice Determines the Population of the Peripheral Naive T Cell Compartment
    Authors: MJ Lang, M Mori, J Ruer-Laven, J Pieters
    J. Immunol., 2017-08-18;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Macrophages Induce Long-Term Trapping of ?? T Cells with Innate-like Properties within Secondary Lymphoid Organs in the Steady State
    Authors: A Audemard-V, M Rivière, A Durand, E Peranzoni, V Guichard, P Hamon, N Bonilla, T Guilbert, A Boissonnas, C Auffray, G Eberl, B Lucas, B Martin
    J. Immunol., 2017-08-04;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Thymic progenitors of TCR??(+) CD8?? intestinal intraepithelial lymphocytes require RasGRP1 for development
    Authors: DP Golec, RE Hoeppli, LM Henao Cavi, J McCann, MK Levings, TA Baldwin
    J. Exp. Med., 2017-06-26;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Lymphatic endothelial S1P promotes mitochondrial function and survival in naive T cells
    Authors: A Mendoza, V Fang, C Chen, M Serasinghe, A Verma, J Muller, VS Chaluvadi, ML Dustin, T Hla, O Elemento, JE Chipuk, SR Schwab
    Nature, 2017-05-24;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  20. CD8?? intraepithelial lymphocytes arise from two main thymic precursors
    Authors: R Ruscher, RL Kummer, YJ Lee, SC Jameson, KA Hogquist
    Nat. Immunol., 2017-05-22;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  21. Ageing adversely affects the migration and function of marginal zone B cells
    Authors: VM Turner, NA Mabbott
    Immunology, 2017-05-04;151(3):349-362.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  22. Histone H3 Lysine 27 demethylases Jmjd3 and Utx are required for T-cell differentiation
    Authors: Sugata Manna, Jong Kyong Kim, Catherine Baugé, Margaret Cam, Yongmei Zhao, Jyoti Shetty et al.
    Nature Communications
  23. IL-17-Producing Vgamma4+ gammadelta T Cells Require Sphingosine 1-Phosphate Receptor 1 for Their Egress from the Lymph Nodes under Homeostatic and Inflammatory Conditions.
    Authors: Maeda Y, Seki N, Kataoka H, Takemoto K, Utsumi H, Fukunari A, Sugahara K, Chiba K
    J Immunol, 2015-07-13;195(4):1408-16.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  24. Mir-17-92 regulates bone marrow homing of plasma cells and production of immunoglobulin G2c.
    Authors: Xu, Shengli, Ou, Xijun, Huo, Jianxin, Lim, Kristen, Huang, Yuhan, Chee, Sheena, Lam, Kong-Pen
    Nat Commun, 2015-04-17;6(0):6764.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  25. Control of lymphocyte egress from lymph nodes through beta2-adrenergic receptors.
    Authors: Nakai A, Hayano Y, Furuta F, Noda M, Suzuki K
    J Exp Med, 2014-11-24;211(13):2583-98.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  26. Moesin controls clathrin-mediated S1PR1 internalization in T cells.
    Authors: Nomachi, Akira, Yoshinaga, Masanori, Liu, Jaron, Kanchanawong, Pakorn, Tohyama, Kiyoshi, Thumkeo, Dean, Watanabe, Takeshi, Narumiya, Shuh, Hirata, Takako
    PLoS ONE, 2013-12-16;8(12):e82590.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  27. Visualization of splenic marginal zone B-cell shuttling and follicular B-cell egress.
    Authors: Arnon, Tal I, Horton, Robert M, Grigorova, Irina L, Cyster, Jason G
    Nature, 2012-12-23;493(7434):684-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  28. The sphingosine 1-phosphate receptor S1P(2) maintains the homeostasis of germinal center B cells and promotes niche confinement.
    Authors: Green JA, Suzuki K, Cho B, Willison LD, Palmer D, Allen CD, Schmidt TH, Xu Y, Proia RL, Coughlin SR, Cyster JG
    Nat. Immunol., 2011-06-05;12(7):672-80.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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