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Mouse S1P1/EDG-1 PE-conjugated Antibody

Catalog #: FAB7089P
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB7089P-100UG
Detection of S1P1/EDG‑1 in HEK293 Human Cell Line Transfected with Mouse S1P1/EDG-1 and eGFP by Flow Cytometry.
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Citations (10)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse S1P1/EDG-1 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse S1P1/EDG‑1 peptide in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 713412
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse S1P1/EDG‑1 synthetic peptide (T4-H28)
Accession # O08530
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of S1P<SUB>1</SUB>/EDG-1 antibody in HEK293 Human Cell Line Transfected with Mouse S1P<SUB>1</SUB>/EDG-1 and eGFP antibody by Flow Cytometry. View Larger

Detection of S1P1/EDG‑1 in HEK293 Human Cell Line Transfected with Mouse S1P1/EDG-1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse S1P1/EDG-1 and eGFP was stained with either (A) Rat Anti-Mouse S1P1/EDG-1 PE-conjugated Monoclonal Antibody (Catalog # FAB7089P) or (B) Rat IgG2APhycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry Detection of S1P<SUB>1</SUB>/EDG-1 antibody in Mouse Thymocytes antibody by Flow Cytometry. View Larger

Detection of S1P1/EDG‑1 in Mouse Thymocytes by Flow Cytometry. Mouse thymocytes were stained with Rat Anti-Mouse CD4 APC-conjugated Monoclonal Antibody (Catalog # FAB554A) and either (A) Rat Anti-Mouse S1P1/EDG-1 PE-conjugated Monoclonal Antibody (Catalog # FAB7089P) or (B) Rat IgG2APhycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: S1P1/EDG-1

S1P1 (sphingosine 1-phosphate receptor-1), also known as EDG-1 (endothelial differentiation, G-protein coupled receptor-1) or S1PR1 (sphingosine-1-phosphate receptor 1) is a widely expressed, 37-40 kDa, G protein coupled receptor within the S1P subfamily of the EDG family. S1P1 is one of five receptors for the bioactive lipid S1P and mediates most of S1P effects on angiogenesis, vascular maturation, and cell migration, especially T cell egress from lymphoid organs. Human and mouse S1P1 share 84% amino acid identity within the N-terminal extracellular portion used as an immunogen.

Long Name
Endothelial Differentiation Gene 1
Entrez Gene IDs
1901 (Human); 13609 (Mouse)
Alternate Names
CD363 antigen; CD363; CHEDG1; D1S3362; EDG1; EDG-1; EDG1edg-1; Endothelial differentiation G-protein coupled receptor 1; endothelial differentiation, sphingolipid G-protein-coupled receptor, 1; FLJ58121; S1P receptor 1; S1P receptor Edg-1; S1P1; S1P1ECGF1; S1PR1; sphingosine 1-phosphate receptor 1; sphingosine 1-phosphate receptor EDG1; Sphingosine 1-phosphate receptor Edg-1; sphingosine-1-phosphate receptor 1

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Citations for Mouse S1P1/EDG-1 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Crosstalk between pro-survival sphingolipid metabolism and complement signaling induces inflammasome-mediated tumor metastasis
    Authors: Janneh AH, Kassir MF, Atilgan FC et al.
    Cell Reports
  2. Sphingosine-1-Phosphate Alleviates Irradiation Induced Salivary Gland Hypofunction through Preserving Endothelial Cells and Resident Macrophages
    Authors: Tao Yang, Qingguo Zhao, Meijun Hu, Simin Pan, Linying Zhang, Ruoxi Zhu et al.
    Antioxidants (Basel)
  3. Neutral ceramidase-dependent regulation of macrophage metabolism directs intestinal immune homeostasis and controls enteric infection
    Authors: R Sun, X Gu, C Lei, L Chen, S Chu, G Xu, MA Doll, Y Tan, W Feng, L Siskind, CJ McClain, Z Deng
    Cell Reports, 2022-03-29;38(13):110560.
    Species: Mouse
    Sample Types: Whole Cell, Whole Cells
    Applications: Flow Cytometry
  4. NLRP3 priming due to skin damage precedes LTP allergic sensitization in a mouse model
    Authors: D Pazos-Cast, Z Gonzalez-K, AY Montalvo, G Hernandez-, A Romero-Sah, V Esteban, M Garrido-Ar, J Tome-Amat, A Diaz-Peral
    Scientific Reports, 2022-02-28;12(1):3329.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Macroautophagy in lymphatic endothelial cells inhibits T cell–mediated autoimmunity
    Authors: Guillaume Harlé, Camille Kowalski, Juan Dubrot, Dale Brighouse, Gaëlle Clavel, Robert Pick et al.
    Journal of Experimental Medicine
  6. Fluvastatin-Pretreated Donor Cells Attenuated Murine aGVHD by Balancing Effector T Cell Distribution and Function under the Regulation of KLF2
    Authors: K Zhao, Y Tian, J Wang, C Chen, B Pan, Z Yan, S Zhu, K Xu
    BioMed Research International, 2020-12-19;2020(0):7619849.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Pro-Survival Lipid Sphingosine-1-Phosphate Metabolically Programs T Cells to Limit Anti-tumor Activity
    Authors: Paramita Chakraborty, Silvia G. Vaena, Krishnamurthy Thyagarajan, Shilpak Chatterjee, Amir Al-Khami, Shanmugam Panneer Selvam et al.
    Cell Reports
  8. Role of the S1P pathway and inhibition by fingolimod in preventing hemorrhagic transformation after stroke
    Authors: A Salas-Perd, F Miró-Mur, M Gallizioli, VH Brait, C Justicia, A Meissner, X Urra, A Chamorro, AM Planas
    Sci Rep, 2019-06-05;9(1):8309.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. CCR2 Signal Facilitates Thymic Egress by Priming Thymocyte Responses to Sphingosine-1-Phosphate
    Authors: Abudureyimujiang Aili, Jie Zhang, Jia Wu, Haoming Wu, Xiuyuan Sun, Qihua He et al.
    Frontiers in Immunology
  10. Normal Thymocyte Egress, T Cell Trafficking, and CD4(+) T Cell Homeostasis Require Interactions between RGS Proteins and G?i2
    Authors: IY Hwang, C Park, K Harrison, JH Kehrl
    J. Immunol, 2017-02-24;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

  1. Can staining with Mouse S1P1/EDG-1 conjugated Antibody, catalog # FAB7089*,  be carried out at 4°C? 

    • There is evidence that G protein family proteins require staining at room temperature (RT) or even 37 °C, so this is what we would recommend.  Staining for 45 minutes is optimal with this antibody. 

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