Mouse S1P5/EDG-8 Alexa Fluor® 350-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: S1P5/EDG-8

S1P₅ (Sphingosine-1 Phosphate receptor 5), also known as EDG-8 (Endothelial Differentiation Gene 8), is a 43-45 kDa member of the EDG family, S1P-binding subfamily of GPCRs. Along with S1P₁ -S1P₄ (or EDG-1, EDG-5, EDG-3 and EDG-6, respectively), S1P₅ is known to bind S1P, a lipid synthesized by platelets, neutrophils, smooth muscle cells, mast cells and select fibroblasts. Mouse S1P₅ is a 400 amino acid (aa) 7-transmembrane glycoprotein that is expressed on brain endothelium, renal mesangial cells, Ly6C⁺ (in human CD14⁺CD16^-) monocytes, pre- and mature oligodendrocytes, and CD27^-CD116⁺ (mature) NK cells. S1P₅ appears to play a role in cell trafficking. On monocytes and NK cells, S1P₅ promotes cell migration out of the bone marrow, a phenomenon that may not be related to an S1P concentration gradient. S1P₅ also helps maintain the integrity of the blood-brain-barrier. On oligodendrocytes and precursors, S1P₅ appears to regulate cell survival and pseudopod extension. The extracellular segment of mouse S1P₅ shares 96% and 94% aa sequence identity with rat and human S1P₅ extracellular domains, respectively.

Product Datasheets

Product Specific Notices

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Related Research Areas

• Bioactive Lipid G Protein-Coupled Receptors
• Bioactive Lipids and Receptors
• Endothelial Cell Differentiation Markers
• Endothelial Cell Markers
• Endothelial Progenitor and Endothelial Cell Markers
• G Protein-Coupled Receptors in Immune Responses
• Steroid Hormones and Bioactive Lipids