Mouse SDNSF/MCFD2 Biotinylated Antibody

Catalog #: BAF2556 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
BAF2556

All SDNSF/MCFD2 Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse SDNSF/MCFD2 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse SDNSF/MCFD2 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse SDNSF/MCFD2
Met1-Gln145
Accession # Q8K5B2

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse SDNSF/MCFD2

Immunohistochemistry

5-15 µg/mL

Immersion fixed frozen sections of embryonic mouse brain

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SDNSF/MCFD2

SDNSF, also known as MCFD2 (multiple coagulation factor deficiency 2), was described as a secreted molecule from adult hippocampal neural stem/progenitor cells (ANSC) that functions as an autocrine/paracrine factor to maintain neurogenesis in the central nervous system. It is also a component of the MCDF2-LMAN1 (Mannose-binding lectin-1, also known as ERGIC-53) complex that functions as a specific cargo receptor for the ER to golgi transport of proteins. Mutations in MCFD2 causes factor 5 and factor 8 combined deficiency. Human SDNSF shares 84% and 82% amino acid sequence identity with rat and mouse SDNSF, respectively.

Long Name

Stem Cell-derived Neuronal Stem/Progenitor Cell Supporting Factor

Entrez Gene IDs

90411 (Human); 193813 (Mouse)

Alternate Names

DKFZp686G21263; F5F8D2; LMAN1IP; MCFD2; multiple coagulation factor deficiency 2; multiple coagulation factor deficiency protein 2; neural stem cell derived neuronal survival protein; Neural stem cell-derived neuronal survival protein; SDNSF; SDNSFF5F8D