Mouse Semaphorin 4A APC-conjugated Antibody

Catalog #: FAB8166A Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB8166A

Detection of Semaphorin 4A in HEK293 Human Cell Line Transfected with Human Semaphorin 4A and eGFP by Flow Cytometry.

1 Image

Product Details

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Semaphorin 4A APC-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Semaphorin 4A in ELISA. Stains HEK293 cells transfected with mouse Semaphorin 4A by Flow Cytometry, but does not stain untransfected cells.

Source

Monoclonal Rat IgG₁ Clone # 757129

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Semaphorin 4A
Thr33-Trp638
Accession # Q62178

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of Semaphorin 4A in HEK293 Human Cell Line Transfected with Human Semaphorin 4A and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse Semaphorin 4A and eGFP was stained with either (A) Rat Anti-Mouse Semaphorin 4A APC-conjugated Monoclonal Antibody (Catalog # FAB8166A) or (B) Rat IgG₁Allophycocyanin Isotype Control (Catalog # IC005A). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Semaphorin 4A

Semaphorin 4A (Sema4A, previously semB) is type I transmembrane protein that is a Class 4 transmembrane Semaphorin with activity in the immune and nervous systems. It is expressed by dendritic cells, monocytes, T cells, B cells, astrocytes and oligodendrocytes, plus visceral smooth muscle and bronchial epithelial cells. The 760 amino acid (aa) mouse Sema4A contains a 650 aa extracellular domain (ECD, aa 33-682) with Sema, PSI and C2-type immunoglobulin domains. It interacts with B and D1 Plexins and lymphocyte TIM-2, enhancing dendritic cell-mediated T cell priming and Th1 responses. Sema4A interaction with endothelial cell plexin-D1 downregulates the actions of VEGF. Mutations of Sema4A are associated with retinitis pigmentosa and cone rod dystrophy in humans. Mouse Sema4A ECD shares 87% and 94% aa sequence identity with human and rat Sema4A, respectively, and shares approximately 35% aa identity with other Sema4 family members. Using this antibody, Sema4A has been shown to interact with Neuropilin-1 in vitro and in vivo to potentiate Treg cell function and survival.

References

Delgoffe, G.M. et al (2013) Nature 501:252.

Entrez Gene IDs

64218 (Human); 20351 (Mouse); 310630 (Rat)

Alternate Names

CORD10; RP35; Sema B; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4A; SEMA4A; SEMAB; Semaphorin 4A; semaphorin-4A; semaphorin-B; SEMB; SEMBFLJ12287