Mouse Semaphorin 4C PE-conjugated Antibody

Name: Mouse Semaphorin 4C PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB8497P

Catalog #: FAB8497P Datasheet / COA / SDS

Discontinued Product

FAB8497P has been discontinued.

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Detection of Semaphorin 4C in HEK293 Human Cell Line Transfected with Mouse Semaphorin 4C and eGFP by Flow Cytometry.

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Mouse Semaphorin 4C PE-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects HEK293 human embryonic cell line transfected with mouse Semaphorin 4C by flow cytometry. Does not detect untransfected or irrelevant transfected HEK293 cells.

Source

Monoclonal Rat IgG₁ Clone # 663203

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myelona cell line NS0-derived recombinant mouse Semaphorin 4C
Glu22-Gly664
Accession # Q64151

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of Semaphorin 4C in HEK293 Human Cell Line Transfected with Mouse Semaphorin 4C and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with mouse Semaphorin 4C and eGFP was stained with either (A) Rat Anti-Mouse Semaphorin 4C PE-conjugated Monoclonal Antibody (Catalog # FAB8497P) or (B) Rat IgG₁Phycoerythrin Isotype Control (Catalog # IC005P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: Semaphorin 4C

Sema4C (Semaphorin 4C; also Sema I and M-SemaF) is a 100-105 kDa, class IV member of the semaphorin family of proteins. It is expressed by precursors to neurons and myocytes, and may regulate their differentiation into mature forms. Mature mouse Sema4C is a type I transmembrane glycoprotein that is 814 amino acids (aa) in length. It contains a 644 aa extracellular region (aa 21-664) that is characterized by the presence of one Sema domain (aa 30-497), a PSI region (aa 499‑552), and an Ig-like C2-type domain (aa 557-645). The cytoplasmic region interacts with PZD-domain containing proteins. Over aa 21-664, mouse Sema4C shares 85% and 95% aa identity with human and rat Sema4C, respectively.

Entrez Gene IDs

54910 (Human); 20353 (Mouse); 301346 (Rat)

Alternate Names

FLJ20369; KIAA1739; MGC126382; MGC126383; M-Sema F; M-SEMA-F; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and shortcytoplasmic domain, (semaphorin) 4C; SEMA4C; SEMACL1; Semaf; SEMAI; Semaphorin 4C; semaphorin-4C

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

FAQs

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