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Mouse Semaphorin 6A Antibody

Catalog #: AF1615
7 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1615
AF1615-SP
Detection of Semaphorin 6A in Mouse Intestine.
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Citations (7)
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Mouse Semaphorin 6A Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Semaphorin 6A in direct ELISAs and Western blots. In Western blots, approximately 25% cross‑reactivity with recombinant human (rh) Semaphorin 6A is observed and less than 1% cross-reactivity with rhSemaphorin 3A, rhSemaphorin 3B, recombinant mouse (rm) Semaphorin 3C, rmSemaphorin 3F, and rmSemaphorin 7A is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse Semaphorin 6A
Gly19-Thr649
Accession # O35464
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Semaphorin 6A
Immunohistochemistry
1-15 µg/mL
Immersion fixed paraffin-embedded sections of Mouse Intestine

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry View Larger

Detection of Semaphorin 6A in Mouse Intestine. Semaphorin 6A was detected in immersion fixed paraffin-embedded sections of Mouse Intestine using Goat Anti-Mouse Semaphorin 6A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1615) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to intestinal glands. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 6A

Semaphorin 6A is a transmembrane protein belonging to the semaphorin family. It functions as an axon guidance signal (1).

References
  1. Fiore, R. and A.W. Puschel (2003) Frontiers Biosci. 8:484.
Entrez Gene IDs
57556 (Human); 20358 (Mouse)
Alternate Names
KIAA1368HT018; sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A; Sema VIA; SEMA; Sema6A; SEMA6A1; SEMA6A-1; Semaphorin 6A; semaphorin 6A-1; Semaphorin VIA; semaphorin-6A; semaphorin-6A-1; SEMAQ; VIA

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Citations for Mouse Semaphorin 6A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Plexin-B2 controls the timing of differentiation and the motility of cerebellar granule neurons
    Authors: Van Battum E, Heitz-Marchaland C, Zagar Y Et al.
    eLife
  2. SEMA6A drives GnRH neuron-dependent puberty onset by tuning median eminence vascular permeability
    Authors: Lettieri, A;Oleari, R;van den Munkhof, MH;van Battum, EY;Verhagen, MG;Tacconi, C;Spreafico, M;Paganoni, AJJ;Azzarelli, R;Andre', V;Amoruso, F;Palazzolo, L;Eberini, I;Dunkel, L;Howard, SR;Fantin, A;Pasterkamp, RJ;Cariboni, A;
    Nature communications
    Species: Mouse, Transgenic Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  3. Recognition of Semaphorin Proteins by P. sordellii Lethal Toxin Reveals Principles of Receptor Specificity in Clostridial Toxins
    Authors: Hunsang Lee, Greg L. Beilhartz, Iga Kucharska, Swetha Raman, Hong Cui, Mandy Hiu Yi Lam et al.
    Cell
  4. MicroRNA miR-27b rescues bone marrow-derived angiogenic cell function and accelerates wound healing in type 2 diabetes mellitus.
    Authors: Wang J, Tao J, Chen D, Cai J, Irani K, Wang Q, Yuan H, Chen A
    Arterioscler Thromb Vasc Biol, 2013-10-31;34(0):99.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Semaphorin 6A regulates angiogenesis by modulating VEGF signaling.
    Authors: Segarra, Marta, Ohnuki, Hidetaka, Maric, Dragan, Salvucci, Ombretta, Hou, Xu, Kumar, Anil, Li, Xuri, Tosato, Giovanna
    Blood, 2012-09-24;120(19):4104-15.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  6. Cis interaction between Semaphorin6A and Plexin-A4 modulates the repulsive response to Sema6A.
    Authors: Haklai-Topper L, Mlechkovich G, Savariego D
    EMBO J., 2010-07-06;29(15):2635-45.
    Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
    Sample Types: Whole Cells
    Applications: ICC
  7. Matrix-binding vascular endothelial growth factor (VEGF) isoforms guide granule cell migration in the cerebellum via VEGF receptor Flk1.
    Authors: Ruiz de Almodovar C, Coulon C, Salin PA, Knevels E, Chounlamountri N, Poesen K, Hermans K, Lambrechts D, Van Geyte K, Dhondt J, Dresselaers T, Renaud J, Aragones J, Zacchigna S, Geudens I, Gall D, Stroobants S, Mutin M, Dassonville K, Storkebaum E, Jordan BF, Eriksson U, Moons L, D'Hooge R, Haigh JJ, Belin MF, Schiffmann S, Van Hecke P, Gallez B, Vinckier S, Chedotal A, Honnorat J, Thomasset N, Carmeliet P, Meissirel C
    J. Neurosci., 2010-11-10;30(45):15052-66.

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