Mouse Serpin D1/Heparin Cofactor II Antibody

Catalog #: AF1265 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1265
AF1265-SP

All Serpin D1/Heparin Cofactor II Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Serpin D1/Heparin Cofactor II Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Serpin D1/Heparin Cofactor II in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Serpin D1/Heparin Cofactor II
Glu24-Ser478
Accession # P49182

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Serpin D1/Heparin Cofactor II (Catalog # 1265-PI)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Mouse Serpin D1/
Heparin Cofactor II (Catalog # 1265-PI), see our available Western blot detection antibodies

Neutralization

Measured by its ability to neutralize Recombinant Mouse Serpin D1 (0.475 µg/mL, Catalog # 1265-PI) inhibition of Recombinant Human Coagulation Factor II/Thrombin (0.05 µg/mL, Catalog # 1473-SE) cleavage of the fluorogenic peptide substrate Boc-VPR-AMC (100 µM, Catalog # ES011). The Neutralization Dose (ND₅₀) is typically 1.23 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin D1/Heparin Cofactor II

Serpin D1 is a member of the Serpin superfamily of the serine protease inhibitors (1). Similar to Serpins A5 and C1, it inhibits thrombin and this activity is enhanced by heparin. Interestingly, a C-terminal His-tagged recombinant Serpin D1 had enhanced heparin effect, which was maintained in a plasma-based thrombin inhibition assay (2). Congenital Serpin D1 deficiency is a potential risk factor for thrombosis (3). Mouse Serpin D1 is synthesized as a 478 amino acid precursor with a 23 amino acid signal peptide (4). The mature chain (residues 24 to 478) is secreted.

References

Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
Bauman, S.J. et al. (1999) J. Biol. Chem. 274:34556.
Tollefsen, D.M. (2002) Arch. Pathol. Lab. Med. 126:1394.
Zhang, G.S. et al. (1994) Biochemistry 33:3632.

Entrez Gene IDs

3053 (Human); 15160 (Mouse)

Alternate Names

HC2; HC2LS2; HCF2; HCF2clade D (heparin cofactor), member 1; HC-II; Heparin Cofactor II; HLS2HCII; leuserpin 2; LS2; Protease inhibitor leuserpin-2; Serpin D1; serpin peptidase inhibitor, clade D (heparin cofactor), member 1