Mouse Serpin I1 Antibody

Catalog #: AF5054 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5054
AF5054-SP

All Serpin I1 Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Serpin I1 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse Serpin I1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human (rh) Serpin I1 is observed and less than 5% cross‑reactivity with rhSerpin I2is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse Serpin I1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse Serpin I1

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin I1

Serpin I1 (serine proteinase inhibitor-clade I 1; also protease inhibitor 17 and neuroserpin) is a 52 kDa member of the serpin superfamily of protease inhibitors. It is a component of dense-core secretory granules in neurons of the cerebellum, hippocampus and hypothalamus and following release shows inhibitory activity towards tissue-type plasminogen activator. The mouse Serpin I1 precursor is 410 amino acids (aa) in length. It contains a 16 aa signal sequence plus a reactive center loop that lies between Gly348 and Pro375. Mature mouse Serpin I1 (aa 17‑410) shares 88% and 92% aa identity with human and rat Serpin I1, respectively.

Entrez Gene IDs

5274 (Human); 20713 (Mouse); 116459 (Rat)

Alternate Names

DKFZp781N13156; Neuroserpin; Peptidase inhibitor 12; PI12; PI-12; PI12Serpin I1; serine (or cysteine) proteinase inhibitor, clade I (neuroserpin), member 1; Serpin I1; serpin peptidase inhibitor, clade I (neuroserpin), member 1