Home / Siglec-1/CD169 / Mouse Siglec-1/CD169 Antibody

Mouse Siglec-1/CD169 Antibody

Catalog #: MAB5610
6 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB5610
MAB5610-SP
Detection of Mouse Siglec‑1/CD169 by Western Blot.
2 Images
Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse Siglec-1/CD169 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Siglec-1/CD169 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Siglec-1,
-5, -6, -7, -8, -9, -10, -11, -14, recombinant mouse Siglec-2, -3, -E, -F, -G, or -H is observed.
Source
Monoclonal Rat IgG2A Clone # 645608
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Siglec-1/CD169
Thr20-Leu1639 (predicted)
Accession # Q62230
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
RAW 264.7 mouse monocyte/macrophage cells stimulated with 1ug/ml LPS overnight 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Siglec-1/CD169 antibody by Western Blot. View Larger

Detection of Mouse Siglec‑1/CD169 by Western Blot. Western blot shows lysates of mouse splenocytes. PVDF Membrane was probed with 1 µg/mL of Mouse Siglec-1/CD169 Monoclonal Antibody (Catalog # MAB5610) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (HAF005). A specific band was detected for Siglec-1/CD169 at approximately 180 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry View Larger

Detection of Siglec‑1/CD169 in RAW264 cells by Flow Cytometry. RAW264 cells stimulated with 1 ug/mL LPS overnight were stained with Rat Anti-Mouse Siglec‑1/CD169 Monoclonal Antibody (Catalog # MAB5610, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Siglec-1/CD169

Siglec-1, also known as sialoadhesin or CD169, is a 175‑185 kDa type I transmembrane glycoprotein belonging to the Siglec family of sialic acid specific I-type lectins within the immunoglobulin superfamily. Mouse Siglec-1 contains a 1619 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set domain and 16 Ig-like C2-set domains. The ECD shares 73% and 83% aa sequence identity with human and rat Siglec-1, respectively. Alternate splicing generates two soluble isoforms containing either 16 or the first 3 Ig-like domains. Siglec-1 is expressed by some tissue macrophages, dendritic cells and circulating monocytes during certain infections. It binds sialylated molecules including MMR, MGL1/CD301a, MUC1, PSGL-1 and CD43.

Long Name
Sialic Acid Binding Ig-like Lectin 1
Entrez Gene IDs
6614 (Human); 20612 (Mouse); 311426 (Rat)
Alternate Names
CD169; FLJ00051; sialic acid binding Ig-like lectin 1, sialoadhesin; sialoadhesin; Siglec1; Siglec-1

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Citations for Mouse Siglec-1/CD169 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Siglec1 suppresses antiviral innate immune response by inducing TBK1 degradation via the ubiquitin ligase TRIM27.
    Authors: Zheng Q, Hou J, Zhou Y, Yang Y, Xie B, Cao X
    Cell Res, 2015-09-11;25(10):1121-36.
  2. Targeting Lymph Node Sinus Macrophages to Inhibit Lymph Node Metastasis
    Authors: Junqing Hu, Jinhao Xu, Mingyue Li, Yanping Zhang, Huaiqiang Yi, Jiangning Chen et al.
    Molecular Therapy - Nucleic Acids
  3. Induction of Siglec-1 by Endotoxin Tolerance Suppresses the Innate Immune Response by Promoting TGF-?1 Production
    Authors: Y Wu, C Lan, D Ren, GY Chen
    J. Biol. Chem., 2016-04-18;291(23):12370-82.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Type I IFN-Inducible Downregulation of MicroRNA-27a Feedback Inhibits Antiviral Innate Response by Upregulating Siglec1/TRIM27.
    Authors: Zheng Q, Hou J, Zhou Y, Yang Y, Cao X
    J Immunol, 2015-12-23;196(3):1317-26.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Immunoactivation induced by chronic viral infection inhibits viral replication and drives immunosuppression through sustained IFN-I responses
    Eur. J. Immunol., 2015-11-17;46(2):372-80.
    Species: Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-Fr
  6. The role of host and microbial factors in the pathogenesis of pneumococcal bacteraemia arising from a single bacterial cell bottleneck.
    Authors: Gerlini A, Colomba L, Furi L, Braccini T, Manso A, Pammolli A, Wang B, Vivi A, Tassini M, Van Rooijen N, Pozzi G, Ricci S, Andrew P, Koedel U, Moxon E, Oggioni M
    PLoS Pathog, 2014-03-20;10(3):e1004026.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Mouse Siglec-1/CD169 Antibody

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Mouse Siglec-1/CD169 Antibody
By Anonymous on 01/24/2021
Application: WB Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line Species: Mouse

RAW 264.7 cell lysates were prepared in lysis buffer (20 mM Tris-HCl, 150 mM NaCl, 1% Triton X-100, pH 7.6, including protease inhibitors, 1
ug/ml leupeptin, 1 ug/ml aprotinin, and 1 mM phenylmethylsulfonyl fluoride).

Western Blot Mouse Siglec-1/CD169 Antibody MAB5610
Mouse Siglec-1/CD169 Antibody
By Oonagh Paerewijck on 02/02/2018
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Spleen tissue Species: Mouse

rat anti-Siglec
+ goat-anti-rat

Immunocytochemistry/Immunofluorescence Mouse Siglec-1/CD169 Antibody MAB5610