Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry

Cited:

Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Slit2 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Slit2
Gln26-Gln900
Accession # Q9R1B9

Specificity

Detects mouse Slit2 in direct ELISAs. In direct ELISAs, less than 50% cross-reactivity with recombinant human (rh) Slit2, rhSlit1 and recombinant mouse (rm) Slit1 is observed, less than 10% cross-reactivity with rmSlit3 and rhSlit3 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse Slit2 Antibody

Slit2 antibody in Embryonic Mouse Hindlimb by Immunohistochemistry (IHC-Fr).

Slit2 in Embryonic Mouse Hindlimb.

Slit2 was detected in immersion fixed frozen sections of E13.5 mouse hindlimb, shown localized to interdigital region (upper panel), using Sheep Anti-Mouse Slit2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5444) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Mouse Slit2 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse hindlimb (E13.5), localized to interdigital region

Reviewed Applications

Read 1 review rated 5 using AF5444 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Slit2

Slit2 is a 180-210 kDa secreted member of the SLIT family of proteins. It is expressed in select sites in the embryo (via glia, motoneurons and posterior sclerotome mesoderm), and found in discrete adult cell types such as preosteoblasts, monocytes, granulose lutein cells, and likely keratinocytes. Slit2 binds to multiple receptors, including ROBO-1 thru -4, laminin-1, Dan, Gremlin and netrin-1. Depending upon the target, Slit2 can promote a number of diverse effects, including both growth cone collapse and outgrowth, inhibition of dendritic cell migration, and axon repulsion. Mature mouse Slit2 is 1496 amino acids (aa) in length (aa 26-1521). It contains multiple intermingled domains, including nine EGF-like domains, 20 Leu-rich repeats (LRRs), one laminin G-like and CTCK (C-terminal Cys knot-like) domain, and eight total C- plus N-terminal LRRs. There are two potential isoform splice variants. One contains a four aa insertion after Ser258, while another possesses the same insertion after Ser258 coupled to both an eight aa insertion after Ser479 and a nine aa insertion after Thr1021. Slit2 apparently undergoes proteolytic cleavage after Arg1113. This generates a 140-150 kDa N-terminal protein, and a 55-60 kDa C-terminal fragment. This processing does not inactivate Slit2. Rather, it creates molecules with distinct activities. For example, the N-terminal fragment will bind ROBO-1 and repel motor axon migration, while the C-terminal fragment won't bind ROBO-1, but will bind glypican-1 and promote motor axon migration. Over aa 26-900, mouse SLIT2 shares 99% and 97%aa sequence identity with rat and human Slit2, respectively.

Long Name

SLIT Homolog 2

Alternate Names

SLIL3

Entrez Gene IDs

9353 (Human); 20563 (Mouse); 360272 (Rat)

Gene Symbol

SLIT2

UniProt

Q9R1B9 (Mouse)

Additional Slit2 Products

Product Documents for Mouse Slit2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Slit2 Antibody

For research use only

Citations for Mouse Slit2 Antibody

Customer Reviews for Mouse Slit2 Antibody (1)

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  • Mouse Slit2 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 10/04/2019
    We used this antibody for a sandwich ELISA format in combination with mAb (5444)) and protein (5444-SL). This combination works well for detecting SLIT2 in human serum.
    Mouse Slit2 Antibody AF5444

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