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Mouse SLPI Antibody

Catalog #: AF1735
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1735
AF1735-SP
Detection of Mouse SLPI by Western Blot.
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Citations (4)
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Mouse SLPI Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse SLPI in direct ELISAs and Western blots. In these formats, less than 5% cross-reactivity with recombinant human SLPI is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived mouse SLPI
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
Mouse ovary

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Mouse SLPI by Western Blot. Western Blot shows lysates of mouse ovary. PVDF membrane was probed with 2 µg/ml of Goat Anti-Mouse SLPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1735) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for SLPI at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SLPI

SLPI is a member of the Serpin family and is primarily expressed in secretory glandular epithelial cells. It is found in various secretory fluids and functions as a potent inhibitor of many proteolytic enzymes. SLPI can modulate cell proliferation and has been shown to have anti-inflammatory, anti-viral, and anti-bacterial activities (1, 2).

References
  1. Zhang, D. et al. (2002) J. Biol. Chem. 277:29999.
  2. Taggart, C.C. et al. (2002) J. Biol. Chem. 277:33648.
Long Name
Secretory Leukocyte Protease Inhibitor
Entrez Gene IDs
6590 (Human); 20568 (Mouse)
Alternate Names
ALK1; ALPProtease inhibitor WAP4; antileukoproteinase; BLPISeminal proteinase inhibitor; HUSI; HUSI-I; Mucus proteinase inhibitor; secretory leukocyte peptidase inhibitor; secretory leukocyte protease inhibitor (antileukoproteinase); Secretory leukocyte protease inhibitor; SLPI; WAP four-disulfide core domain protein 4; WAP4HUSI-1; WFDC4MPI

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Citations for Mouse SLPI Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. SLPI is a critical mediator that controls PTH-induced bone formation
    Authors: A Morimoto, J Kikuta, K Nishikawa, T Sudo, M Uenaka, M Furuya, T Hasegawa, K Hashimoto, H Tsukazaki, S Seno, A Nakamura, D Okuzaki, F Sugihara, A Ninomiya, T Yoshimura, R Takao-Kawa, H Matsuda, M Ishii
    Nature Communications, 2021-04-09;12(1):2136.
  2. MIF-2/D-DT enhances proximal tubular cell regeneration through SLPI- and ATF4-dependent mechanisms
    Authors: Akinobu Ochi, Dong Chen, Wibke Schulte, Lin Leng, Nickolas Moeckel, Marta Piecychna et al.
    American Journal of Physiology-Renal Physiology
  3. Increased secretory leukocyte protease inhibitor (SLPI) production by highly metastatic mouse breast cancer cells.
    Authors: Sayers, Kevin T, Brooks, Alan D, Sayers, Thomas J, Chertov, Oleg
    PLoS ONE, 2014-08-11;9(8):e104223.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Secretory leukoprotease inhibitor in mucosal lymph node dendritic cells regulates the threshold for mucosal tolerance.
    Authors: Samsom JN, van der Marel AP, van Berkel LA, van Helvoort JM, Simons-Oosterhuis Y, Jansen W, Greuter M, Nelissen RL, Meeuwisse CM, Nieuwenhuis EE, Mebius RE, Kraal G
    J. Immunol., 2007-11-15;179(10):6588-95.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot

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