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Mouse SOD3/EC-SOD Antibody

Catalog #: AF4817
10 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF4817
AF4817-SP
Detection of Mouse SOD3/EC‑SOD by Western Blot.
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Citations (10)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse SOD3/EC-SOD Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse SOD3/EC‑SOD in Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse SOD1 or SOD2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse SOD3/EC‑SOD
Ser25-Thr251
Accession # O09164
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
20 µg/mL
Mouse aorta tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse SOD3/EC‑SOD antibody by Western Blot. View Larger

Detection of Mouse SOD3/EC‑SOD by Western Blot. Western blot shows lysates of mouse lung and kidney tissue. PVDF membrane was probed with 1 µg/mL Goat Anti-Mouse SOD3/EC-SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4817) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant mouse SOD1, SOD2, and SOD3 (5 ng/lane) were included. A specific band for SOD3/EC-SOD was detected at approxi-mately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western View Larger

Detection of Mouse SOD3/EC‑SOD by Simple WesternTM. Simple Western shows lysates of mouse aorta, loaded at 0.5 mg/ml. A specific band was detected for SOD3/EC‑SOD at approximately 40 kDa (as indicated) using 20 µg/mL of Goat Anti-Mouse SOD3/EC‑SOD Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4817). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SOD3/EC-SOD

Superoxide Dismutases (SODs), originally identified as Indophenoloxidase (IPO), are enzymes that catalyze the conversion of naturally-occuring but harmful superoxide radicals into molecular oxygen and hydrogen peroxide. Superoxide Dismutases 3, SOD3, also known as extracellular (EC) SOD, is tetrameric glycoprotein with an apparent subunit molecular weight of about 30 kDa. Like SOD1, SOD3 binds one Cu2+ and Zn2+ ions per subunit but differs in sequence and tissue distribution. Three isoenzymes of SOD have been identified and are functionally related but have very modest sequence homology. SOD3 shares 23% and 17% sequence identity with SOD1 and SOD2, respectively. Mouse SOD3 shares ~66% and 82% sequence homology with human and rat SOD3, respectively. SOD3 is a secretory protein and is synthesized with a putative 24-amino acid signal peptide preceding the 227 amino acids in the mature SOD3. SOD3 is found in plasma, lymph, and synovial fluid as well as in tissues. SOD3 binds on the surface of endothelial cells through the heparan sulfate proteoglycan and eliminates the oxygen radicals from the NADP-dependent oxidative system of neutrophils.

Long Name
Superoxide Dismutase-3
Entrez Gene IDs
6649 (Human); 20657 (Mouse); 25352 (Rat)
Alternate Names
ECSOD; EC-SOD; EC-SODEC 1.15.1.1; extracellular superoxide dismutase [Cu-Zn]; MGC20077; SOD3; superoxide dismutase 3, extracellular

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Citations for Mouse SOD3/EC-SOD Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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  1. Cancer-induced Cardiac Atrophy Adversely Affects Myocardial Redox State and Mitochondrial Oxidative Characteristics
    Authors: Lee DE, Brown JL, Rosa-Caldwell ME et al.
    JCSM Rapid Communications
  2. TLR4-Mediated Inflammatory Responses Regulate Exercise-Induced Molecular Adaptations in Mouse Skeletal Muscle
    Authors: H Fujiyoshi, T Egawa, E Kurogi, T Yokokawa, K Kido, T Hayashi
    International Journal of Molecular Sciences, 2022-02-07;23(3):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  3. Cancer-induced Cardiac Atrophy Adversely Affects Myocardial Redox State and Mitochondrial Oxidative Characteristics
    Authors: Lee DE, Brown JL, Rosa-Caldwell ME et al.
    JCSM Rapid Communications
  4. Comparative plasma proteomics in muscle atrophy during cancer-cachexia and disuse: The search for atrokines
    Authors: S Lim, KR Dunlap, ME Rosa-Caldw, WS Haynie, LT Jansen, TA Washington, NP Greene
    Physiol Rep, 2020-10-01;8(19):e14608.
    Species: Mouse
    Sample Types: Plasma
    Applications: Western Blot
  5. Cathepsin A contributes to left ventricular remodeling by degrading extracellular superoxide dismutase in mice
    Authors: M Hohl, M Mayr, L Lang, AG Nickel, J Barallobre, X Yin, T Speer, SR Selejan, C Goettsch, K Erb, C Fecher-Tro, JC Reil, B Linz, S Ruf, T Hübschle, C Maack, M Böhm, T Sadowski, D Linz
    J. Biol. Chem., 2020-07-09;0(0):.
    Species: Mouse
    Sample Types: Cell Culture Lysates
    Applications: Western Blot
  6. Long-term voluntary running prevents the onset of symptomatic Friedreich's ataxia in mice
    Authors: H Zhao, BM Lewellen, RJ Wilson, D Cui, JC Drake, M Zhang, Z Yan
    Sci Rep, 2020-04-08;10(1):6095.
    Species: Mouse
    Sample Types: Tissue Homogenate
    Applications: Western Blot
  7. Pretreatment with statin attenuates the cardiotoxicity of Doxorubicin in mice.
    Authors: Riad A, Bien S, Westermann D, Becher PM, Loya K, Landmesser U, Kroemer HK, Schultheiss HP, Tschope C
    Cancer Res., 2009-01-15;69(2):695-9.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  8. Mitochondrial degeneration precedes the development of muscle atrophy in progression of cancer cachexia in tumour-bearing mice
    Authors: Brown JL, Rosa-Caldwell ME, Lee DE et al.
    J Cachexia Sarcopenia Muscle.
  9. Mitochondrial Function and Protein Turnover in the Diaphragm are Altered in LLC Tumor Model of Cancer Cachexia
    Authors: Megan E. Rosa-Caldwell, Conner A. Benson, David E. Lee, Jacob L. Brown, Tyrone A. Washington, Nicholas P. Greene et al.
    International Journal of Molecular Sciences
  10. Extracellular Superoxide Dismutase Protects against Proteinuric Kidney Disease
    Authors: Roderick J. Tan, Dong Zhou, Liangxiang Xiao, Lili Zhou, Yingjian Li, Sheldon I. Bastacky et al.
    Journal of the American Society of Nephrology

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