Mouse ST2/IL-33R Quantikine ELISA Kit Summary
Product Summary
Recovery
The recovery of mouse ST2 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Supernates (n=4) | 98 | 81-111 |
| EDTA Plasma (n=4) | 104 | 92-118 |
| Heparin Plasma (n=4) | 94 | 86-103 |
| Serum (n=4) | 103 | 89-113 |
Linearity
Scientific Data
Product Datasheets
Preparation and Storage
Background: ST2/IL-33R
ST2, also known as IL-1 R4, is a component of the receptor for the proinflammatory cytokine Interleukin-33. The IL-33 receptor additionally includes IL-1 RAcp which also associates with IL-1 RI and IL-1 R rp2/IL-1 R6. A soluble form of ST2 functions as a decoy receptor. IL-33 promotes Th2-biased immune responses, resulting in eosinophilia, mast cell activation, and allergic inflammation. In addition, IL-33 enhances inflammation-associated hypernociception and protects from atherosclerosis and cardiac myocyte hypertrophy. Soluble ST2 is elevated in the serum under inflammatory conditions including allergic asthma, sepsis, trauma, dengue fever, pulmonary disease, and lupus. It is also associated aortic stenosis, congestive cardiomyopathy, and risk of cardiovascular heart failure and death.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Assay Diluent to each well.
- Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.
- Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 5 times.
- Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Mouse ST2/IL-33R Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
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IL-33 induces thymic involution-associated naive T cell aging and impairs host control of severe infection
Authors: L Xu, C Wei, Y Chen, Y Wu, X Shou, W Chen, D Lu, H Sun, W Li, B Yu, X Wang, X Zhang, Y Yu, Z Lei, R Tang, J Zhu, Y Li, L Lu, H Zhou, S Zhou, C Su, X Chen
Nature Communications, 2022-11-12;13(1):6881.
Species: Mouse
Sample Types: Serum
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Cancer cell-derived interleukin-33 decoy receptor sST2 enhances orthotopic tumor growth in a murine pancreatic cancer model
Authors: K Takenaga, M Akimoto, N Koshikawa, H Nagase
PLoS ONE, 2020-04-27;15(4):e0232230.
Species: Mouse
Sample Types: Serum
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Rorc restrains the potency of ST2+ regulatory T cells in ameliorating intestinal graft-versus-host disease
Authors: J Yang, A Ramadan, DK Reichenbac, M Loschi, J Zhang, B Griesenaue, H Liu, KL Hippen, BR Blazar, S Paczesny
JCI Insight, 2019-03-07;0(0):.
Species: Mouse
Sample Types: Plasma
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From proteomics to discovery of first-in-class ST2 inhibitors active in vivo
Authors: AM Ramadan, E Daguindau, JC Rech, K Chinnaswam, J Zhang, GL Hura, B Griesenaue, Z Bolten, A Robida, M Larsen, JA Stuckey, CY Yang, S Paczesny
JCI Insight, 2018-07-26;3(14):.
Species: Mouse
Sample Types: Plasma
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Effects of carvedilol on structural and functional outcomes and plasma biomarkers in the mouse transverse aortic constriction heart failure model
Authors: C Hampton, R Rosa, D Szeto, G Forrest, B Campbell, R Kennan, S Wang, CH Huang, L Gichuru, X Ping, X Shen, K Small, J Madwed, JJ Lynch
SAGE Open Med, 2017-03-30;5(0):2050312117700.
Species: Mouse
Sample Types: Plasma
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Soluble IL-33 receptor sST2 inhibits colorectal cancer malignant growth by modifying the tumour microenvironment
Nat Commun, 2016-11-24;7(0):13589.
Species: Mouse
Sample Types: Cell Culture Supernates
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Fibrates ameliorate the course of bacterial sepsis by promoting neutrophil recruitment via CXCR2.
Authors: Tancevski I, Nairz M, Duwensee K, Auer K, Schroll A, Heim C, Feistritzer C, Hoefer J, Gerner R, Moschen A, Heller I, Pallweber P, Li X, Theurl M, Demetz E, Wolf A, Wolf D, Eller P, Ritsch A, Weiss G
EMBO Mol Med, 2014-04-06;6(6):810-20.
Species: Mouse
Sample Types: Cell Culture Supernates
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Vasoactive intestinal peptide downregulates proinflammatory TLRs while upregulating anti-inflammatory TLRs in the infected cornea.
Authors: Jiang X, McClellan SA, Barrett RP, Zhang Y, Hazlett LD
J. Immunol., 2012-06-01;189(1):269-78.
Species: Mouse
Sample Types: Tissue Homogenates
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Dendritic cell-specific transcript: dendritic cell marker and regulator of TLR-induced cytokine production.
Authors: Hontelez S, Ansems M, Karthaus N
J. Immunol., 2012-05-21;189(1):138-45.
Species: Mouse
Sample Types: Tissue Homogenates
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