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Mouse TCR gamma/delta PE-conjugated Antibody

Catalog #: FAB7297P Datasheet / COA / SDS

Discontinued Product

FAB7297P has been discontinued.
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Detection of TCR gamma /δ in Mouse Splenocytes by Flow Cytometry.
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Mouse TCR gamma/delta PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TCR gamma /δ.
Source
Monoclonal Hamster IgG2, kappa Clone # GL-3/5E11
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse intraepithelial lymphocytes
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TCR ?/d antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of TCR gamma /δ in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC-conjugated Monoclonal Antibody (Catalog # FAB4841A) and either (A) Hamster Anti-Mouse TCR ?/d PE-conjugated Monoclonal Antibody (Catalog # FAB7297P) or (B) Hamster IgG Phycoerythrin Control. View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: TCR gamma/delta

The gamma δ T-cell receptor (TCR) is a heteromer that includes type I transmembrane CD3 gamma and CD3δ glycoprotein subunits of the Ig superfamily. T gamma δ cells develop as a minor population in the thymus and migrate mainly to skin and intestinal epithelial layers. Mouse and rat CD3 gamma and CD3δ are synthesized as 182 and 173 amino acid (aa) precursors that result in 160 and 152 aa mature proteins with 94 and 84 aa extracellular domains (ECD), respectively. The germline ECD sequences of CD3 gamma and CD3δ share 71% and 76% aa identity between mouse and rat, respectively, while both proteins and species share 57-62% aa identity with human CD3 gamma and CD3δ. Mouse intraepithelial lymphocytes from the small intestine, which contain a major population of gamma δ T cells, were used as the immunogen for the GL-3/5E11 antibody(1).

References
  1. Goodman, T. and L. Lefrancois (1989) J. Exp. Med. 170:2401569.
Entrez Gene IDs
915 (Human); 12500 (Mouse); 25710 (Rat)
Alternate Names
delta polypeptide; T cell receptor gamma locus; T3D; T-cell antigen receptor, gamma polypeptide; T-cell rearranging gene, gamma; T-cell receptor, gamma cluster; TCR gamma/delta; TCRG; TiT3; TRG

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