Mouse TER-119 Fluorescein-conjugated Antibody

Catalog #: FAB1125F Datasheet / COA / SDS

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FAB1125F-025

Detection of TER‑119 in Mouse Bone Marrow Cells by Flow Cytometry.

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Mouse TER-119 Fluorescein-conjugated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TER‑119 in Western blots. This antibody has been shown to react with cells of the erythroid lineage in embryonic yolk sac, fetal liver, adult bone marrow, and adult peripheral blood.

Source

Monoclonal Rat IgG_2B Clone # TER-119

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

C57BL/6 mouse day-14 fetal liver cells

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Fluorescein (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of TER‑119 in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Rat Anti-Mouse B220/CD45R PE-conjugated Monoclonal Antibody (FAB1217P) and either (A) Rat Anti-Mouse TER-119 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1125F) or (B) Rat IgG2BFluorescein Isotype Control (IC013F). View our protocol for Staining Membrane-associated Proteins.

Flow Cytometry

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Detection of TER‑119 in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Rat Anti-Mouse CD45 PE-conjugated Monoclonal Antibody (Catalog # FAB114P) and either (A) Rat Anti-Mouse TER-119 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1125F) or (B) Rat IgG_2BFluorescein Isotype Control (Catalog # IC013F). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: TER-119

The monoclonal antibody TER-119 is isolated from a hybridoma generated using splenocytes from a rat subcutaneously injected with day 14 BALB/c fetal liver cells (1). The TER-119 monoclonal antibody reacts with erythroid cells from the early proerythroblast to mature erythrocyte stages of development (1). The 52 kDa ligand for TER-119 is associated with glycophorin A on erythrocytes (1). TER-119 antibodies are frequently used in combination with other lineage depletion antibodies to enrich for mouse hematopoietic stem cells (2, 3).

References

Kina, T. et al. (2000) Br. J. Haematol. 109:280.
Ikuta, K. et al. (1990) Cell 62:863.
Osawa, M.Y. et al., (1996) Hematopoietic Stem Cells in Weir’s Handbook of Experimental Immunology, Vol. 2, 5th Edition. Herzenberg, L.A. et al. eds. Blackwell Science, Cambridge, MA. pp. 66.1-66.5

Entrez Gene IDs

104231 (Mouse)

Alternate Names

Ly76; Ly-76; TER119; TER-119