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Mouse TER-119 PE-conjugated Antibody

Catalog #: FAB1125P
2 Citations Datasheet / COA / SDS
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FAB1125P

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Detection of TER‑119 in Balb/c Mouse Bone Marrow Cells by Flow Cytometry.
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Mouse TER-119 PE-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TER‑119 in Western blots. This antibody has been shown to react with cells of the erythroid lineage in embryonic yolk sac, fetal liver, adult bone marrow, adult peripheral blood, and adult lymphoid organs (Ikuta, K. et al. (1990) Cell 62:863). TER-119 Antigen is a molecule associated with cell-surface glycophorin A (Kina, T. et al. (2000) Br. J. Haematol. 109:280). The antibody has been shown to react with erythroid cells at differentiation stages from early proerythroblast to mature erythrocyte (Kina, T. et al. (2000) Br. J. Haematol. 109:280).
Source
Monoclonal Rat IgG2B Clone # TER-119
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
C57BL/6 mouse day-14 fetal liver cells
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of TER-119 antibody in Balb/c Mouse Bone Marrow Cells antibody by Flow Cytometry. View Larger

Detection of TER‑119 in Balb/c Mouse Bone Marrow Cells by Flow Cytometry. Balb/c mouse bone marrow cells was stained with Rat Anti-Mouse TER-119 PE-conjugated Monoclonal Antibody (Catalog # FAB1125P) and Rat Anti-Mouse B220/CD45R Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB1217F). Quadrant markers were set based on control antibody staining. View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: TER-119

The monoclonal antibody TER-119 is isolated from a hybridoma generated using splenocytes from a rat subcutaneously injected with day 14 BALB/c fetal liver cells (1). The TER-119 monoclonal antibody reacts with erythroid cells from the early proerythroblast to mature erythrocyte stages of development (1). The 52 kDa ligand for TER-119 is associated with glycophorin A on erythrocytes (1). TER-119 antibodies are frequently used in combination with other lineage depletion antibodies to enrich for mouse hematopoietic stem cells (2, 3).

References
  1. Kina, T. et al. (2000) Br. J. Haematol. 109:280.
  2. Ikuta, K. et al. (1990) Cell 62:863.
  3. Osawa, M.Y. et al., (1996) Hematopoietic Stem Cells in Weir’s Handbook of Experimental Immunology, Vol. 2, 5th Edition. Herzenberg, L.A. et al. eds. Blackwell Science, Cambridge, MA. pp. 66.1-66.5
Entrez Gene IDs
104231 (Mouse)
Alternate Names
Ly76; Ly-76; TER119; TER-119

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Citations for Mouse TER-119 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Regulation of bile duct epithelial injury by hepatic CD71+ erythroid cells
    Authors: L Yang, P Shivakumar, JM Kinder, SS Way, B Donnelly, R Mourya, Z Luo, JA Bezerra
    JCI Insight, 2020-06-04;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Infection with Anaplasma phagocytophilum induces multilineage alterations in hematopoietic progenitor cells and peripheral blood cells.
    Authors: Johns JL, Macnamara KC, Walker NJ, Winslow GM, Borjesson DL
    Infect. Immun., 2009-06-29;77(9):4070-80.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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