Mouse TFPI-2 Antibody

Catalog # Availability Size / Price Qty
MAB4045
MAB4045-SP
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Mouse TFPI-2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TFPI-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant mouse TFPI or recombinant human TFPI-2 is observed.
Source
Monoclonal Rat IgG2B Clone # 375717
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TFPI-2
Leu23-Lys211
Accession # O35536
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse TFPI‑2 under non-reducing conditions only
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse TFPI‑2, see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TFPI-2

TFPI-2 (tissue factor pathway inhibitor 2) is a secreted protein that inhibits several serine proteases. The amino acid sequence of mouse TFPI-2 is 86%, 58% and 54% identical to that of rat, human/chimpanzee and canine.

Long Name
Tissue Factor Pathway Inhibitor 2
Entrez Gene IDs
7980 (Human)
Alternate Names
Placental protein 5; PP5; PP5FLJ21164; REF-1; TFPI2; TFPI-2; TFPI-2REF1; tissue factor pathway inhibitor 2

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