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Mouse TIM-1/KIM-1/HAVCR Antibody

Catalog #: MAB1817
22 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1817-SP
MAB1817-500
MAB1817-100
Detection of TIM‑1/KIM‑1/HAVCR in HEK293 cells transfected with Mouse TIM-1 by Flow Cytometry
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Citations (22)
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Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TIM-1/KIM-1/HAVCR Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TIM‑1/KIM-1/HAVCR in ELISAs and Western blots. This antibody does not cross-react with recombinant human (rh) TIM‑1, rmTIM-2, or rmTIM-3.
Source
Monoclonal Rat IgG2B Clone # 222414
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TIM‑1/KIM-1/HAVCR
Tyr22-Thr212
Accession # NP_001160104
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse TIM‑1/KIM‑1/HAVCR (Catalog # 1817-TM)
Flow Cytometry
0.25 µg/106 cells
HEK293 cells transfected with Mouse Tim-1

Mouse TIM-1/KIM-1/HAVCR Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse TIM‑1/KIM‑1/HAVCR Biotinylated Antibody (Catalog # BAF1817)

Standard: Recombinant Mouse TIM-1/KIM-1/HAVCR Protein (Catalog # 1817-TM)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of TIM‑1/KIM‑1/HAVCR in HEK293 cells transfected with Mouse TIM-1 by Flow Cytometry HEK293 cells transfected with Mouse TIM-1 were stained with Rat Anti-Mouse TIM‑1/KIM‑1/HAVCR Monoclonal Antibody (Catalog # MAB1817, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram) followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-1/KIM-1/HAVCR

TIM-1 (T cell-immunoglobulin-mucin; also known as KIM-1 or HAVCR) is a 70-80 kDa, type I transmembrane glycoprotein member of the TIM family of immunoglobulin superfamily molecules (1-4). This gene family is involved in the regulation of Th1 and Th2-cell-mediated immunity. In mouse, there are eight known TIM genes (# 1-8) vs. only three genes in human (# 1, 3, and 4) (1, 2). Mouse TIM-1 and -2 are counterparts of human TIM-1 while mouse TIM-5 through 8 have no human counterparts (2). Mouse TIM-1 is synthesized as a 305 amino acid (aa) precursor that contains a 21 aa signal sequence, a 216 aa extracellular domain (ECD), a 21 aa transmembrane segment and a 47 aa cytoplasmic domain (5, 6). The ECD contains one V-type Ig-like domain and a mucin region characterized by multiple T-S-P motifs. The mucin region undergoes extensive O-linked glycosylation. The mouse TIM-1 gene is highly polymorphic and, based on rat, may undergo alternate splicing (4, 6). For instance, HBA mice show a 15 aa deletion in the mucin region that occurs in BALB/c mice (6). This difference is associated with a decreased susceptibility to asthma. Other polymorphisms are also documented (6). In human, TIM-1 is known to circulate as a soluble form. It undergoes constitutive cleavage by an undefined MMP, releasing a 75-85 kDa soluble molecule (5). The same thing might be expected in mouse. The ECD of mouse TIM-1 is 50%, 39% and 80% aa identical to human, canine and rat TIM-1 ECD, respectively. The only two reported ligands for TIM-1 are TIM-4 and the hepatitis A virus (8, 9). However, others are believed to exist, and based on the ligand for TIM-3, one possibility might be an S-type lectin (10). TIM-1 ligation induces T cell proliferation and promotes cytokine production (1, 10). In particular, it induces IL-4 production, and requires the cytoplasmic tyrosine phosphorylation motif (5).

References
  1. Meyers, J.H. et al. (2005) Trends Mol. Med. 11:1471. 
  2. Kuchroo, V.K. et al. (2003) Nat. Rev. Immunol. 3:454. 
  3. Mariat, C. et al. (2005) Phil. Trans. R. Soc. B 360:1681.  
  4. Ichimura, T. et al. (1998) J. Biol. Chem. 273:4135.  
  5. de Souza, A.J. et al. (2005) Proc. Natl. Acad. Sci. USA 102:17113. 
  6. McIntire, J.J. et al. (2001) Nat. Immunol. 2:1109.
  7. Bailly, V. et al. (2002) J. Biol. Chem. 277:39739.
  8. Feigelstock, D. et al. (1998) J. Virol. 72:6621.
  9. Zhu, C. et al. (2005) Nat. Immunol. 6:1245.
  10. Meyers, J.H. et al. (2005) Nat. Immunol. 6:455.
Long Name
T Cell Immunoglobulin Mucin-1
Entrez Gene IDs
26762 (Human); 171283 (Mouse); 286934 (Rat); 102141332 (Cynomolgus Monkey)
Alternate Names
CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1

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Citations for Mouse TIM-1/KIM-1/HAVCR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

22 Citations: Showing 1 - 10
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  1. The Role of the EGF Receptor in Sex Differences in Kidney Injury
    Authors: Ming-Zhi Zhang, Kensuke Sasaki, Yan Li, Zhilian Li, Yu Pan, Guan-nan Jin et al.
    Journal of the American Society of Nephrology
  2. EGF Receptor–Dependent YAP Activation Is Important for Renal Recovery from AKI
    Authors: Jianchun Chen, Huaizhou You, Yan Li, You Xu, Qian He, Raymond C. Harris
    Journal of the American Society of Nephrology
  3. Two independent modes of kidney stone suppression achieved by AIM/CD5L and KIM-1
    Authors: Kyohei Matsuura, Natsumi Maehara, Aika Hirota, Ayaka Eguchi, Keisuke Yasuda, Kaori Taniguchi et al.
    Communications Biology
  4. Analyzing cell-type-specific dynamics of metabolism in kidney repair
    Authors: Gangqi Wang, Bram Heijs, Sarantos Kostidis, Ahmed Mahfouz, Rosalie G. J. Rietjens, Roel Bijkerk et al.
    Nature Metabolism
  5. Intrarenal dopamine deficiency leads to hypertension and decreased longevity in mice
    Authors: Ming-Zhi Zhang, Bing Yao, Suwan Wang, Xiaofeng Fan, Guanqing Wu, Haichun Yang et al.
    Journal of Clinical Investigation
  6. Phosphatidylinositol metabolism of the renal proximal tubule S3 segment is disturbed in response to diabetes
    Authors: RGJ Rietjens, G Wang, AIM van der Ve, A Koudijs, MC Avramut, S Kooijman, PCN Rensen, J van der Vl, TJ Rabelink, B Heijs, BM van den Be
    Scientific Reports, 2023-04-17;13(1):6261.
  7. Dipeptidase-1 governs renal inflammation during ischemia reperfusion injury
    Authors: A Lau, JJ Rahn, M Chappellaz, H Chung, H Benediktss, D Bihan, A von Mässen, A Linkermann, CN Jenne, SM Robbins, DL Senger, IA Lewis, J Chun, DA Muruve
    Science Advances, 2022-02-02;8(5):eabm0142.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  8. A combination of monosodium glutamate and high-fat and high-fructose diets increases the risk of kidney injury, gut dysbiosis and host-microbial co-metabolism
    Authors: T Pongking, O Haonon, R Dangtakot, S Onsurathum, A Jusakul, K Intuyod, A Sangka, S Anutrakulc, U Cha'on, S Pinlaor, P Pinlaor
    PLoS ONE, 2020-04-08;15(4):e0231237.
    Species: Hamster
    Sample Types: Whole Tissue
    Applications: IHC
  9. Protective Effects of Recombinant Human Soluble Thrombomodulin on Lipopolysaccharide-Induced Acute Kidney Injury
    Authors: Y Nozaki, J Ri, K Sakai, K Niki, M Funauchi, I Matsumura
    Int J Mol Sci, 2020-04-05;21(7):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  10. Dietary sodium modulates nephropathy in Nedd4-2-deficient mice
    Authors: JA Manning, SS Shah, TL Henshall, A Nikolic, J Finnie, S Kumar
    Cell Death Differ., 2019-12-04;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. Renal immune surveillance and dipeptidase-1 contribute to contrast-induced acute kidney injury
    Authors: A Lau, H Chung, T Komada, JM Platnich, CF Sandall, SR Choudhury, J Chun, V Naumenko, BG Surewaard, MC Nelson, A Ulke-Lemée, PL Beck, H Benediktss, AM Jevnikar, SL Snelgrove, MJ Hickey, DL Senger, MT James, JA Macdonald, P Kubes, CN Jenne, DA Muruve
    J. Clin. Invest., 2018-06-04;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  12. Proximal tubule-derived colony stimulating factor-1 mediates polarization of renal macrophages and dendritic cells, and recovery in acute kidney injury.
    Authors: Wang Y, Chang J, Yao B, Niu A, Kelly E, Breeggemann M, Abboud Werner S, Harris R, Zhang M
    Kidney Int, 2015-09-30;88(6):1274-1282.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. KIM-1-mediated phagocytosis reduces acute injury to the kidney.
    Authors: Yang L, Brooks C, Xiao S, Sabbisetti V, Yeung M, Hsiao L, Ichimura T, Kuchroo V, Bonventre J
    J Clin Invest, 2015-03-09;125(4):1620-36.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: ELISA Development (Capture)
  14. The pathological role of IL-18Ralpha in renal ischemia/reperfusion injury.
    Authors: Yano T, Nozaki Y, Kinoshita K, Hino S, Hirooka Y, Niki K, Shimazu H, Kishimoto K, Funauch M, Matsumura I
    Lab Invest, 2014-10-20;95(1):78-91.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  15. HAVCR/KIM-1 activates the IL-6/STAT-3 pathway in clear cell renal cell carcinoma and determines tumor progression and patient outcome.
    Authors: Cuadros T, Trilla E, Sarro E, Vila M, Vilardell J, de Torres I, Salcedo M, Lopez-Hellin J, Sanchez A, Ramon y Cajal S, Itarte E, Morote J, Meseguer A
    Cancer Res, 2014-01-03;74(5):1416-28.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  16. Endogenous Tim-1 (Kim-1) promotes T-cell responses and cell-mediated injury in experimental crescentic glomerulonephritis.
    Authors: Nozaki Y, Nikolic-Paterson DJ, Snelgrove SL
    Kidney Int., 2011-12-28;81(9):844-55.
    Species: Mouse
    Sample Types: Tissue Homogenates, Urine
    Applications: ELISA Development, Western Blot
  17. T cell Ig domain and mucin domain 1 engagement on invariant NKT cells in the presence of TCR stimulation enhances IL-4 production but inhibits IFN-gamma production.
    Authors: Kim HS, Kim HS, Lee CW, Chung DH
    J. Immunol., 2010-03-10;184(8):4095-106.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. TIM-1 and TIM-3 enhancement of Th2 cytokine production by mast cells.
    Authors: Nakae S, Iikura M, Suto H, Akiba H, Umetsu DT, DeKruyff RH, Saito H, Galli SJ
    Blood, 2007-07-09;110(7):2565-8.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  19. CSF-1 signaling mediates recovery from acute kidney injury
    Authors: Zhang MZ, Yao B, Yang S et al.
    J Clin Invest
  20. Sirtuin 5 Regulates Proximal Tubule Fatty Acid Oxidation to Protect against AKI
    Authors: Takuto Chiba, Kevin D. Peasley, Kasey R. Cargill, Katherine V. Maringer, Sivakama S. Bharathi, Elina Mukherjee et al.
    Journal of the American Society of Nephrology
  21. Deletion of Nedd4-2 results in progressive kidney disease in mice
    Authors: Tanya L Henshall, Jantina A Manning, Omri S Alfassy, Pranay Goel, Natasha A Boase, Hiroshi Kawabe et al.
    Cell Death & Differentiation
  22. IL-4/IL-13-mediated polarization of renal macrophages/dendritic cells to an M2a phenotype is essential for recovery from acute kidney injury.
    Authors: Zhang Ming-Zhi, Wang Xin, Wang Yinqiu et al.
    Kidney International

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Mouse TIM-1/KIM-1/HAVCR Antibody
By Anonymous on 10/13/2021
Application: WB Sample Tested: Kidney tissue Species: Mouse
Western Blot Mouse TIM-1/KIM-1/HAVCR Antibody MAB1817