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Mouse TIM-2 Antibody

Catalog #: MAB1885
2 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1885
MAB1885-SP
Product Details
Citations (2)
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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TIM-2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects human TIM-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human TIM-1, recombinant mouse (rm) TIM-1 or rmTIM-3 is observed.
Source
Monoclonal Rat IgG2A Clone # 222919
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TIM‑2
His22-Asn229
Accession # NP_599010
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse TIM‑2

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-2

TIM-2 is a type I transmembrane protein that has an immunoglobulin variable region-like domain followed by a mucin-like domain in its extracellular region. It is selectively expressed on activated T cells and binds to Semaphorin 4A.

Long Name
T Cell Immunoglobulin Mucin-2
Entrez Gene IDs
171284 (Mouse)
Alternate Names
G9D3; T-cell immunoglobulin and mucin domain-containing protein 2; T-cell immunoglobulin mucin receptor 2; T-cell membrane protein 2; TIM2; TIM-2; TIMD-2

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Citations for Mouse TIM-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Tim-2 is the receptor for H-ferritin on oligodendrocytes.
    Authors: Todorich B, Zhang X, Slagle-Webb B, Seaman WE, Connor JR
    J. Neurochem., 2008-11-05;107(6):1495-505.
    Species: Rat
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Transferrin and H‐ferritin involvement in brain iron acquisition during postnatal development: impact of sex and genotype
    Authors: Brian Chiou, Elizabeth B. Neely, Dillon S. Mcdevitt, Ian A. Simpson, James R. Connor
    Journal of Neurochemistry

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