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Mouse TIM-2 Antibody

Catalog #: AF1885
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF1885
AF1885-SP
TIM‑2 in Mouse Liver.
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Citations (1)
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Mouse TIM-2 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TIM-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 2% cross‑reactivity with recombinant human TIM-1 and recombinant mouse TIM-3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TIM‑2
His22-Asn229
Accession # NP_599010
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse TIM‑2
Immunohistochemistry
1-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry TIM-2 antibody in Mouse Liver by Immunohistochemistry (IHC-Fr). View Larger

TIM‑2 in Mouse Liver. TIM-2 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse TIM-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1885) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sinusoids. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIM-2

TIM-2 is a type I membrane protein and is a member of the TIM protein family. This protein family is characterized by their conserved immunoglobulin and mucin domains, as well as by their expression on activated T cells. TIM-2 is a receptor for Sema 4A, which is expressed on dendritic cells and B cells. Sema 4A has been shown to enhance T cell activation.

Long Name
T Cell Immunoglobulin Mucin-2
Entrez Gene IDs
171284 (Mouse)
Alternate Names
G9D3; T-cell immunoglobulin and mucin domain-containing protein 2; T-cell immunoglobulin mucin receptor 2; T-cell membrane protein 2; TIM2; TIM-2; TIMD-2

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Citation for Mouse TIM-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Neuroimmune semaphorin 4A as a drug and drug target for asthma.
    Authors: Mogie G, Shanks K, Nkyimbeng-Takwi EH et al.
    Int Immunopharmacol

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