Home / TIMP-1 / Mouse TIMP-1 Antibody

Mouse TIMP-1 Antibody

Catalog #: AF980
25 Citations 2 Reviews Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF980
AF980-SP
TIMP-1 in Mouse Ovary.
1 Image
Product Details
Citations (25)
FAQs
Supplemental Products
Reviews (2)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Mouse TIMP-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TIMP-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross-reactivity with recombinant rat TIMP-1 is observed, less than 5% cross-reactivity with recombinant human (rh) TIMP-1 is observed, and less than 1% cross-reactivity with rhTIMP-2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TIMP-1
Cys25-Arg205
Accession # P12032
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse TIMP‑1 Western Blot Standard (Catalog # WBC022)
Immunohistochemistry
5-15 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse TIMP‑1 (Catalog # 980‑MT), see our available Western blot detection antibodies
Neutralization
Measured by its ability to neutralize Recombinant Mouse TIMP-1 (0.07 µg/mL, Catalog # 980-MT) inhibition of Recombinant Mouse/Rat MMP‑2 (0.2 µg/mL, Catalog # 924-MP) cleavage of the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2 (10 µM, Catalog # ES001). The Neutralization Dose (ND50) is typically 1.3 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry TIMP-1 antibody in Mouse Ovary by Immunohistochemistry (IHC-Fr). View Larger

TIMP-1 in Mouse Ovary. TIMP-1 was detected in perfusion fixed frozen sections of mouse ovary using Goat Anti-Mouse TIMP-1 Antigen Affinity-purified Polyclonal Antibody (red; Catalog # AF980) at 15 µg/mL overnight at 4 °C.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Loading...
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TIMP-1

Tissue inhibitors of metalloproteinases or TIMPs are a family of homologous proteins that regulate the activity of matrix metalloproteinases (MMPs) (1, 2). There are four known members of the family, TIMP-1, TIMP-2, TIMP-3, and TIMP-4 that have been found to exhibit multiple functions, including inhibition of active MMPs, pro‑MMP activation, cell growth promotion, matrix binding, inhibition of angiogenesis and the induction of apoptosis. Structurally, TIMPs have two domains, an N‑terminal domain and a C-terminal domain. Each domain consists of three disulfide-bonded loops. TIMP-1 is a glycoprotein produced by a wide range of cell types. Through its N-terminal domain, TIMP-1 inhibits active MMPs by forming a non-covalent binary complex with the MMP active site. The C-terminal domain of TIMP‑1 interacts with the C-terminal domain of pro‑MMP‑9, which may play a role in regulating pro‑MMP‑9 activation.

References
  1. Murphy, G. and F. Willenbrock (1995) Methods Enzymol. 248:496.
  2. Brew, K. et al. (2000) Biochim. Biophys. Acta 1477:267.
Long Name
Tissue Inhibitors of Metalloproteinases 1
Entrez Gene IDs
7076 (Human); 21857 (Mouse); 116510 (Rat)
Alternate Names
CLGI; Collagenase inhibitor; collagenase inhibitor); EPATIMP-1; EPO; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; HCI; metalloproteinase inhibitor 1; TIMP metallopeptidase inhibitor 1; TIMP1; TIMP-1; TIMPtissue inhibitor of metalloproteinase 1 (erythroid potentiating activity; Tissue inhibitor of metalloproteinases 1

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Mouse TIMP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

25 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. Orthologous proteins of experimental de- and remyelination are differentially regulated in the CSF proteome of multiple sclerosis subtypes
    Authors: Nellie A. Martin, Arkadiusz Nawrocki, Viktor Molnar, Maria L. Elkjaer, Eva K. Thygesen, Miklos Palkovits et al.
    PLOS ONE
  2. Ym1+ macrophages orchestrate fibrosis, lesion growth and progression during development of murine pancreatic cancer
    Authors: Fleming Martinez A, DOppler H, Bastea L et al.
    iScience
  3. Elevated Serum Gastrin Is Associated with Melanoma Progression: Putative Role in Increased Migration and Invasion of Melanoma Cells
    Authors: Varga, AJ;Nemeth, IB;Kemeny, L;Varga, J;Tiszlavicz, L;Kumar, D;Dodd, S;Simpson, AWM;Buknicz, T;Beynon, R;Simpson, D;Krenacs, T;Dockray, GJ;Varro, A;
    International journal of molecular sciences
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Western Blot
  4. Regorafenib prevents the development of emphysema in a murine elastase model
    Authors: Kwangseok Oh, Gun-Wu Lee, Han-Byeol Kim, Jin-Hee Park, Eun-Young Shin, Eung-Gook Kim
    BMB Reports
  5. Single cell RNA sequencing analysis of mouse cochlear supporting cell transcriptomes with activated ERBB2 receptor indicates a cell-specific response that promotes CD44 activation
    Authors: D Piekna-Prz, D Na, J Zhang, C Baker, JM Ashton, PM White
    Frontiers in Cellular Neuroscience, 2023-01-06;16(0):1096872.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  6. Enrichment of Newly Synthesized Proteins following treatment of C2C12 Myotubes with Endotoxin and Interferon-gamma
    Authors: Catherine S. Coleman, Bruce A. Stanley, Charles H. Lang
    Inflammation
  7. Matrix metalloproteinases and tissue inhibitors of metalloproteinases in murine beta -coronavirus-induced neuroinflammation
    Authors: Sourodip Sengupta, Sankar Addya, Diptomit Biswas, Paromita Banerjee, Jayasri Das Sarma
    Virology
  8. Myeloid-Derived TSP1 (Thrombospondin-1) Contributes to Abdominal Aortic Aneurysm Through Suppressing Tissue Inhibitor of Metalloproteinases-1
    Authors: Huan Yang, Ting Zhou, Christine M. Sorenson, Nader Sheibani, Bo Liu
    Arteriosclerosis, Thrombosis, and Vascular Biology
  9. CD73+ extracellular vesicles inhibit angiogenesis through adenosine A2B receptor signalling
    Authors: Roberta Angioni, Cristina Liboni, Stephanie Herkenne, Ricardo Sánchez‐Rodríguez, Giulia Borile, Elisabetta Marcuzzi et al.
    Journal of Extracellular Vesicles
  10. In Vivo Activation and Pro-Fibrotic Function of NF-kappaB in Fibroblastic Cells During Pulmonary Inflammation and Fibrosis Induced by Carbon Nanotubes
    Authors: J Dong, Q Ma
    Front Pharmacol, 2019-10-02;10(0):1140.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  11. Deletion of ADAM-9 in HGF/CDK4 mice impairs melanoma development and metastasis
    Authors: N Giebeler, A Schönefu beta, J Landsberg, T Tüting, C Mauch, P Zigrino
    Oncogene, 2017-05-29;0(0):.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. Mouse mesenchymal stem cells inhibit high endothelial cell activation and lymphocyte homing to lymph nodes by releasing TIMP-1
    Authors: L Zanotti, R Angioni, B Calì, C Soldani, C Ploia, F Moalli et al.
    Leukemia
  13. Fibroblast Activation Protein (FAP) Accelerates Collagen Degradation and Clearance from Lungs in Mice
    Authors: MH Fan, Q Zhu, HH Li, HJ Ra, S Majumdar, DL Gulick, JA Jerome, DH Madsen, M Christofid, DW Speicher, WW Bachovchin, C Feghali-Bo, E Puré
    J. Biol. Chem., 2015-12-09;291(15):8070-89.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  14. Low-density lipoprotein receptor-related protein-1 mediates endocytic clearance of tissue inhibitor of metalloproteinases-1 and promotes its cytokine-like activities.
    Authors: Thevenard J, Verzeaux L, Devy J, Etique N, Jeanne A, Schneider C, Hachet C, Ferracci G, David M, Martiny L, Charpentier E, Khrestchatisky M, Rivera S, Dedieu S, Emonard H
    PLoS ONE, 2014-07-30;9(7):e103839.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  15. TAp73 is essential for germ cell adhesion and maturation in testis.
    Authors: Holembowski L, Kramer D, Riedel D, Sordella R, Nemajerova A, Dobbelstein M, Moll U
    J Cell Biol, 2014-03-24;204(7):1173-90.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  16. Tissue inhibitor of matrix metalloproteinases-1 loaded poly(lactic-co-glycolic acid) nanoparticles for delivery across the blood–brain barrier
    Authors: Mayank Chaturvedi, Yves Molino, Sreedhar Sreedhar, Michel Khrestchatisky, Leszek Kaczmarek
    International Journal of Nanomedicine
  17. Angiogenic capacity of M1- and M2-polarized macrophages is determined by the levels of TIMP-1 complexed with their secreted proMMP-9
    Authors: Ewa Zajac, Bernhard Schweighofer, Tatyana A. Kupriyanova, Anna Juncker-Jensen, Petra Minder, James P. Quigley et al.
    Blood
  18. Regulation of PDGFC signalling and extracellular matrix composition by FREM1 in mice
    Authors: Fenny Wiradjaja, Denny L. Cottle, Lynelle Jones, Ian Smyth
    Disease Models & Mechanisms
  19. The MMP-9/TIMP-1 axis controls the status of differentiation and function of myelin-forming Schwann cells in nerve regeneration.
    Authors: Kim Y, Remacle AG, Chernov AV
    PLoS ONE, 2012-03-16;7(3):e33664.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  20. Differential vesicular distribution and trafficking of MMP-2, MMP-9, and their inhibitors in astrocytes.
    Authors: Sbai O, Ould-Yahoui A, Ferhat L, Gueye Y, Bernard A, Charrat E, Mehanna A, Risso JJ, Chauvin JP, Fenouillet E, Rivera S, Khrestchatisky M
    Glia, 2010-02-01;58(3):344-66.
    Species: Mouse
    Sample Types: Cell Lysates, Whole Cells
    Applications: Electron Microscopy, ICC, Western Blot
  21. Amelioration of coxsackievirus B3-mediated myocarditis by inhibition of tissue inhibitors of matrix metalloproteinase-1.
    Authors: Crocker SJ, Frausto RF, Whitmire JK, Benning N, Milner R, Whitton JL
    Am. J. Pathol., 2007-11-30;171(6):1762-73.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  22. Tissue inhibitor of metalloproteinase-1 deficiency amplifies acute lung injury in bleomycin-exposed mice.
    Authors: Kim KH, Burkhart K, Chen P, Frevert CW, Randolph-Habecker J, Hackman RC, Soloway PD, Madtes DK
    Am. J. Respir. Cell Mol. Biol., 2005-06-09;33(3):271-9.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  23. Upregulation of MMP-9/TIMP-1 enzymatic system in eosinophilic meningitis caused by Angiostrongylus cantonensis.
    Authors: Chen KM, Lee HH, Chou HL, Liu JY, Tsai BC, Lai SC
    Int J Exp Pathol, 2005-04-01;86(2):81-9.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Western Blot
  24. TIMP1 expression underlies sex disparity in liver metastasis and survival in pancreatic cancer
    Authors: Chris D. Hermann, Benjamin Schoeps, Celina Eckfeld, Enkhtsetseg Munkhbaatar, Lukas Kniep, Olga Prokopchuk et al.
    Journal of Experimental Medicine
  25. Involvement of TIMP-1 in PECAM-1-mediated tumor dissemination
    Authors: Abraham V, Cao G, Parambath A et al.
    Int J Onc

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Mouse TIMP-1 Antibody

Average Rating: 4.5 (Based on 2 Reviews)

5 Star
50%
4 Star
50%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Mouse TIMP-1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Mouse TIMP-1 Antibody
By Anonymous on 01/25/2024
Application: IHC Sample Tested: Skeletal muscle Species: Mouse

Fresh frozen skeletal muscle tissue from wt mouse (11 months old)

Immunohistochemistry Mouse TIMP-1 Antibody AF980
Mouse TIMP-1 Antibody
By Anonymous on 04/26/2017
Application: Immunocytochemistry/Immunofluorescence Sample Tested: activated mouse CD8 T cell Species: Mouse