Mouse TLR11 Antibody

Catalog #: MAB7640 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7640
MAB7640-SP

Detection of TLR11 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

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All TLR11 Antibodies

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Mouse TLR11 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TLR11 in direct ELISAs.

Source

Monoclonal Rat IgG_2B Clone # 786404

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant mouse TLR11
Thr209-Gln325
Accession # Q6R5P0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of TLR11 antibody in RAW 264.7 Mouse Cell Line antibody by Flow Cytometry.

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Detection of TLR11 in RAW 264.7 Mouse Cell Line by Flow Cytometry. RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Mouse TLR11 Monoclonal Antibody (Catalog # MAB7640, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR11

TLR11 is a type I transmembrane receptor of the Toll-like receptor family that is primarily expressed in epithelial cells, but also in dendritic cells and macrophages. The 926 amino acid (aa) mouse TLR11 transcript encodes a 30 aa signal sequence, a 691 aa extracellular domain with 10 leucine-rich repeats and 9 potential N-glycosylation sites, a 21 aa transmembrane domain, and a 184 aa cytoplasmic domain with a TIR domain. Within the region used as an immunogen, mouse and rat TLR11 share 86% aa sequence identity. Human TLR11 is a pseudogene that is not expressed. TLR11 resides in the endoplasmic reticulum (ER), interacts with the multispan ER protein UNC93B1, recognizes profilin-like proteins on Toxoplasma gondii and other intracellular parasites, and activates dendritic cell IL-12 production.

Long Name

Toll-like Receptor 11

Entrez Gene IDs

239081 (Mouse); 498491 (Rat)

Alternate Names

Gm287; hgnc31753; TLR11; Toll like receptor 11

Product Datasheets

Product Datasheet

COA

SDS

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