Mouse TLR6 Antibody

Catalog #: AF1533
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1533
AF1533-SP

All TLR6 Antibodies

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Citations (1)

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Mouse TLR6 Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse TLR6 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant mouse (rm) TLR1 is observed and less than 1% cross-reactivity with rmTLR2, recombinant human (rh) TLR3 and rhTLR4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse TLR6
Leu43-Asp594
Accession # BAA78632

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse TLR6 Fc Chimera (Catalog # 1533-TR)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TLR6

The Toll-like family of molecules are a group of integral membrane proteins that serve as pattern recognition receptors for microbial pathogens. There are at least eleven mouse and ten human members that activate the innate immune system following exposure to a variety of microbial species (1‑4). All Toll-like receptors (TLRs) are type I transmembrane (TM) proteins that exist either in the plasma membrane or in the membranes of endosomal structures (where they bind intracellular microbial nucleic acids). All TLRs also contain a large number of extracellular leucine-rich repeats (LRRs) and a cytoplasmic tail with a Toll/IL-1 receptor (TIR) domain. The mouse TLR6 cDNA encodes a 795 amino acid (aa) precursor that includes a 27 aa signal sequence, a 557 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 190 aa cytoplasmic domain. The ECD contains 14 Leu-rich repeats, and the cytoplasmic region contains one TIR domain (5). Within the ECD, mouse TLR6 shares 59% aa sequence identity with mouse TLR1 and 20‑27% aa sequence identitity with mouse TLR2, -3, -4, -5, -7, -8, -9, -11, -12, and -13. It shares 71%, 72%, and 86% aa sequence identity with bovine, human, and rat TLR6, respectively. TLR6 is expressed on the cell surface of macrophages, monocytes, neutrophils, and dermal endothelial cells in ligand-independent association with TLR2 (6‑9). TLR2 also associates with TLR1, a functional complex with specificity for distinct but related microbial ligands (6‑8). TLR6 and TLR2 cooperate in the recognition of acylated bacterial and mycoplasma lipopeptides, peptidoglycan, and glycosylphosphatidylinositols (7‑14). The cytoplasmic TIR domain is necessary and sufficient to initiate signal transduction which leads to activation of NF kappa B (7, 15).

References

Hopkins, P.A. and S. Sriskandan (2005) Clin. Exp. Immunol. 140:395.
Roeder, A. et al. (2004) Med. Mycol. 42:485.
Netea, M. et al. (2004) J. Leukoc. Biol. 75:749.
Wetzler, L.M. (2003) Vaccine 21:S55.
Takeuchi, O. et al. (1999) Gene 231:59.
Hajjar, A.M. et al. (2001) J. Immunol. 166:15.
Ozinsky, A. et al. (2000) Proc. Natl. Acad. Sci. USA 97:13766.
Lee, J.Y. et al. (2004) J. Biol. Chem. 279:16971.
Nakao, Y. et al. (2005) J. Immunol. 174:1566.
Bulut, Y. et al. (2001) J. Immunol. 167:987.
Takeuchi, O. et al. (2001) Int. Immunol. 13:933.
Morr, M. et al. (2002) Eur. J. Immunol. 32:3337.
Krishnegowda, G. et al. (2005) J. Biol. Chem. 280:8606.
Omueti, K.O. et al. (2005) J. Biol. Chem. 280:36616.
Nishiya, T. and A.L. DeFranco (2004) J. Biol. Chem. 279:19008.

Long Name

Toll-like Receptor 6

Entrez Gene IDs

10333 (Human); 21899 (Mouse); 305353 (Rat)

Alternate Names

CD286 antigen; CD286; TLR6; toll-like receptor 6

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Citation for Mouse TLR6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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