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Mouse TNF RI/TNFRSF1A Antibody

Catalog #: AF-425-PB
27 Citations Datasheet / COA / SDS
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AF-425-PB
AF-425-SP
Detection of Mouse TNF RI/TNFRSF1A by Western Blot.
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Citations (27)
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Mouse TNF RI/TNFRSF1A Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse TNF RI/TNFRSF1A in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 5% cross-reactivity with recombinant human TNF RI is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse TNF RI
Ile22-Ala212
Accession # P25118
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
5-15 µg/mL
Perfusion fixed frozen sections of mouse intestine
Agonist Activity
Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.
The ED50 for this effect is typically 0.05-0.15 μg/mL.
 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse TNF RI/TNFRSF1A antibody by Western Blot. View Larger

Detection of Mouse TNF RI/TNFRSF1A by Western Blot. Western blot shows lysates of L-929 mouse fibroblast cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Mouse TNF RI/TNFRSF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-425-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TNF RI/TNFRSF1A at approximately 50-55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of TNF RI/TNFRSF1A antibody in L-929 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of TNF RI/TNFRSF1A in L‑929 Mouse Cell Line by Flow Cytometry. L-929 mouse fibroblast cell line was stained with Goat Anti-Mouse TNF RI/TNFRSF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-425-PB, filled histo-gram) or control antibody (Catalog # AB-108-C, open histo-gram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TNF RI/TNFRSF1A

TNF receptor 1 (TNF RI; also called TNF R‑p55/p60, TNFRSF1A and CD120a) is a type I transmembrane protein that belongs to the TNF receptor superfamily (1, 2). TNF RI is widely expressed and is present on the cell surface as a trimer of 55 kDa subunits. It serves as a receptor for both TNF‑ alpha and TNF‑ beta /lymphotoxin. Each subunit contains four TNF‑ alpha trimer‑binding cysteine‑rich domains (CRD) in its extracellular domain (ECD) (1‑6). TNF‑ alpha binding to TNF R1 induces the sequestration of TNFRI in lipid rafts, where it activates NF kappa B and is cleaved by ADAM‑17/TACE (7, 8). Release of the 28‑34 kDa TNF RI ECD occurs constitutively, and in response to products of pathogens such as LPS, CpG DNA or S. aureus protein A (1, 7‑12). Full‑length TNF RI may also be released in exosome‑like vesicles (12). Such release helps to resolve inflammatory reactions as it down‑regulates cell surface TNF RI and provides soluble TNF RI to bind TNF‑ alpha (6, 13, 14). Exclusion from lipid rafts causes endocytosis of TNF RI complexes and induces apoptosis (7, 15). Although there is a second receptor for TNF‑ alpha (TNF R2), TNF RI is thought to mediate most of the cellular effects of TNF‑ alpha (3). TNF R1 is essential for proper development of lymph node germinal centers and Peyer’s patches, and for combating intracellular pathogens such as Listeria monocytogenes (1‑3). Mouse TNF RI is a 454 amino acid (aa) protein that contains a 21 aa signal sequence and a 191 aa ECD with a PLAD domain (6). This mediates constitutive trimer formation. The PLAD domain is followed by four CRDs, a 23 aa transmembrane domain, and a 219 aa cytoplasmic sequence that contains a neutral sphingomyelinase activation domain and a death domain (16). The ECD of mouse TNF RI shows 67%, 70%, 64%, 70% and 88% aa identity with canine, feline, procine, human and rat TNF RI, respectively; and it shows 23% aa identity with the ECD of TNF RII.

References
  1. Pfeffer, K. (2003) Cytokine Growth Factor Rev. 14:185.
  2. Hehlgans, T. and K. Pfeffer (2005) Immunology 115:1.
  3. Peschon, J.J. et al. (1998) J. Immunol. 160:943.
  4. Banner, D.W et al. (1993) Cell 73:431.
  5. Medvedev, A.E. et al. (1996) J. Biol. Chem. 271:9778.
  6. Chan, F.K. et al. (2000) Science 288:2351.
  7. Legler, D.F. et al. (2003) Immunity 18:655.
  8. Tellier, E. et al. (2006) Exp. Cell Res. 312:3969.
  9. Xanthoulea, S. et al. (2004) J. Exp. Med. 200:367.
  10. Jin, L. et al. (2000) J. Immunol. 165:5153.
  11. Gomez, M.I. et al. (2006) J. Biol. Chem. 281:20190.
  12. Islam, A. et al. (2006) J. Biol. Chem. 281:6860.
  13. Garton, K.J. et al. (2006) J. Leukoc. Biol. 79:1105.
  14. McDermott, M.F. et al. (1999) Cell 97:133.
  15. Schneider-Brachert, W. et al. (2004) Immunity 21:415.
  16. Lewis, M. et al. (1991) Proc. Natl. Acad. Sci. USA 88:2830.
Long Name
Tumor Necrosis Factor Receptor I
Entrez Gene IDs
7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
Alternate Names
CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor

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Citations for Mouse TNF RI/TNFRSF1A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

27 Citations: Showing 1 - 10
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  1. Tumor necrosis factor (TNF)-receptor 1 and 2 mediate homeostatic synaptic plasticity of denervated mouse dentate granule cells
    Authors: Denise Becker, Thomas Deller, Andreas Vlachos
    Scientific Reports
  2. TRAPS mutations in Tnfrsf1a decrease the responsiveness to TNF alpha via reduced cell surface expression of TNFR1
    Authors: Takahiko Akagi, Sumie Hiramatsu-Asano, Kenta Ikeda, Hiroyasu Hirano, Shoko Tsuji, Ayano Yahagi et al.
    Frontiers in Immunology
  3. SPATA2 regulates the activation of RIPK1 by modulating linear ubiquitination
    Authors: Ran Wei, Lily Wen Xu, Jianping Liu, Yanxia Li, Pei Zhang, Bing Shan et al.
    Genes & Development
  4. Dimerization and Ubiquitin Mediated Recruitment of A20, a Complex Deubiquitinating Enzyme
    Authors: Timothy T. Lu, Michio Onizawa, Gianna E. Hammer, Emre E. Turer, Qian Yin, Ermelinda Damko et al.
    Immunity
  5. In vivo single-cell CRISPR uncovers distinct TNF programmes in tumour evolution
    Authors: Renz, PF;Ghoshdastider, U;Baghai Sain, S;Valdivia-Francia, F;Khandekar, A;Ormiston, M;Bernasconi, M;Duré, C;Kretz, JA;Lee, M;Hyams, K;Forny, M;Pohly, M;Ficht, X;Ellis, SJ;Moor, AE;Sendoel, A;
    Nature
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: Immunohistochemistry
  6. CMTM4 is a subunit of the IL-17 receptor and mediates autoimmune pathology
    Authors: D Knizkova, M Pribikova, H Draberova, T Semberova, T Trivic, A Synackova, A Ujevic, J Stefanovic, A Drobek, M Huranova, V Niederlova, O Tsyklauri, A Neuwirth, J Tureckova, O Stepanek, P Draber
    Nature Immunology, 2022-10-21;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. PP6 negatively modulates LUBAC-mediated M1-ubiquitination of RIPK1 and c-FLIPL to promote TNFalpha-mediated cell death
    Authors: G Wu, D Li, W Liang, W Sun, X Xie, Y Tong, B Shan, M Zhang, X Lu, J Yuan, Y Li
    Cell Death & Disease, 2022-09-07;13(9):773.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation, Western Blot
  8. ANKRD13a controls early cell-death checkpoint by interacting with RIP1 independent of NF-kappaB
    Authors: M Won, KA Park, S Kim, E Ju, Y Ko, H Yoo, H Ro, J Lee, J Oh, EG Lee, SY Kim, SW Nam, HM Shen, MK Yeo, JM Kim, GM Hur
    Cell Death and Differentiation, 2021-11-27;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  9. A20 prevents inflammasome-dependent arthritis by inhibiting macrophage necroptosis through its ZnF7 ubiquitin-binding domain
    Authors: A Polykratis, A Martens, RO Eren, Y Shirasaki, M Yamagishi, Y Yamaguchi, S Uemura, M Miura, B Holzmann, G Kollias, M Armaka, G van Loo, M Pasparakis
    Nat. Cell Biol., 2019-05-13;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: IP Western
  10. Death-domain dimerization-mediated activation of RIPK1 controls necroptosis and RIPK1-dependent apoptosis
    Authors: H Meng, Z Liu, X Li, H Wang, T Jin, G Wu, B Shan, DE Christoffe, C Qi, Q Yu, Y Li, J Yuan
    Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Sorafenib tosylate inhibits directly necrosome complex formation and protects in mouse models of inflammation and tissue injury
    Authors: S Martens, M Jeong, W Tonnus, F Feldmann, S Hofmans, V Goossens, N Takahashi, JH Bräsen, EW Lee, P Van der Ve, J Joossens, K Augustyns, S Fulda, A Linkermann, J Song, P Vandenabee
    Cell Death Dis, 2017-06-29;8(6):e2904.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  12. Endogenous TNF? orchestrates the trafficking of neutrophils into and within lymphatic vessels during acute inflammation
    Authors: S Arokiasamy, C Zakian, J Dilliway, W Wang, S Nourshargh, MB Voisin
    Sci Rep, 2017-03-13;7(0):44189.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Transforming growth factor beta-activated kinase 1 signaling pathway critically regulates myocardial survival and remodeling.
    Authors: Li L, Chen Y, Doan J, Murray J, Molkentin J, Liu Q
    Circulation, 2014-10-02;130(24):2162-72.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Therapy of peritoneally disseminated colon cancer by TAP-deficient embryonic stem cell-derived macrophages in allogeneic recipients.
    Authors: Haga E, Endo Y, Haruta M, Koba C, Matsumura K, Takamatsu K, Ikeda T, Nishimura Y, Senju S
    J Immunol, 2014-07-16;193(4):2024-33.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. Anti-inflammatory cytokines directly inhibit innate but not adaptive CD8+ T cell functions.
    Authors: Freeman B, Meyer C, Slifka M
    J Virol, 2014-04-16;88(13):7474-84.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Pericytes support neutrophil subendothelial cell crawling and breaching of venular walls in vivo.
    Authors: Proebstl D, Voisin MB, Woodfin A, Whiteford J, D'Acquisto F, Jones GE, Rowe D, Nourshargh S
    J. Exp. Med., 2012-05-21;209(6):1219-34.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  17. TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice.
    Authors: Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM
    J. Clin. Invest., 2009-09-14;119(10):2954-64.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  18. IL-1beta augments TNF-alpha-mediated inflammatory responses from lung epithelial cells.
    Authors: Saperstein S, Chen L, Oakes D, Pryhuber G, Finkelstein J
    J Interferon Cytokine Res, 2009-05-01;29(5):273-84.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Complex Application
  19. The function of TRADD in signaling through tumor necrosis factor receptor 1 and TRIF-dependent Toll-like receptors.
    Authors: Pobezinskaya YL, Kim YS, Choksi S, Morgan MJ, Li T, Liu C, Liu Z
    Nat. Immunol., 2008-07-20;9(9):1047-54.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  20. P2Y1 receptor-evoked glutamate exocytosis from astrocytes: control by tumor necrosis factor-alpha and prostaglandins.
    Authors: Domercq M, Brambilla L, Pilati E, Marchaland J, Volterra A, Bezzi P
    J. Biol. Chem., 2006-08-01;281(41):30684-96.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  21. Thyrocytes isolated from autoimmune-diseased thyroids secrete soluble tumor necrosis factor-R1 that is related to their elevated protein kinase C activity.
    Authors: LaBue M, Colburn KK, Green LM
    Thyroid, 2004-04-01;14(4):249-62.
    Species: Mouse
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  22. Chaperone-mediated autophagy is involved in the execution of ferroptosis
    Authors: Z Wu, Y Geng, X Lu, Y Shi, G Wu, M Zhang, B Shan, H Pan, J Yuan
    Proc. Natl. Acad. Sci. U.S.A., 2019-02-04;0(0):.
  23. TAB2 deficiency induces dilated cardiomyopathy by promoting RIPK1-dependent apoptosis and necroptosis
    Authors: Haifeng Yin, Xiaoyun Guo, Yi Chen, Yachang Zeng, Xiaoliang Mo, Siqi Hong et al.
    Journal of Clinical Investigation
  24. ABIN-1 regulates RIPK1 activation by linking Met1 ubiquitylation with Lys63 deubiquitylation in TNF-RSC
    Authors: SA Dziedzic, Z Su, V Jean Barre, A Najafov, AK Mookhtiar, P Amin, H Pan, L Sun, H Zhu, A Ma, DW Abbott, J Yuan
    Nat. Cell Biol., 2017-12-04;0(0):.
  25. Selective inhibition of the p38 alternative activation pathway in infiltrating T cells inhibits pancreatic cancer progression
    Authors: Muhammad S. Alam, Matthias M. Gaida, Frank Bergmann, Felix Lasitschka, Thomas Giese, Nathalia A. Giese et al.
    Nature Medicine
  26. Identification of a novel mechanism of blood-brain communication during peripheral inflammation via choroid plexus-derived extracellular vesicles
    EMBO Mol Med, 2016-10-04;0(0):.
  27. Pellino3 targets RIP1 and regulates the pro-apoptotic effects of TNF-alpha
    Authors: Shuo Yang, Bingwei Wang, Lisa S. Tang, Jakub Siednienko, John J. Callanan, Paul N. Moynagh
    Nature Communications

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